RegulonDB RegulonDB 10.8: Operon Form
   

mtlADR operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: mtlADR
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: mtlADR
Synonym(s): OP00271
Gene(s): mtlA, mtlD, mtlR   Genome Browser M3D Gene expression COLOMBOS
Note(s): Two palindromic sequences downstream of the transcriptional start site of mtlA were determined by Figge et al. (1994) Figge RM,1994 Those authors proposed that these sequences may function as a binding site for MtlR. This was also determined by gene expression analysis Figge RM,1994 However, Tan et al. (2009) 19840941detected, based on in vitro DNA-binding studies, that there was no interaction of MtlR with the well-characterized mannitol operator/promoter region. Tan and colleagues proposed that this regulation is indirect, through the involvement of some other DNA-binding regulator 19840941
The expression of the mtlADR operon is induced in the presence of mannitol and inhibited in the presence of glucose, in an MtlR-HPr-dependent way 31558743.
Based on the manner of regulation of the mtlADR operon, the operon is considered one of the most complex found in bacteria Ramseier TM,1995
Evidence: [LTED] Length of transcript experimentally determined
Reference(s): [1] Figge RM., et al., 1994
[2] Lee CA., et al., 1983
Promoter
Name: mtlAp
+1: 3772188
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 93
Sequence: tgatatcacacaaaaggccgtcgactggacagttaaccgattcagtgccagatttcgcagTatctacaaggtccggctacc
                          -35                    -10        +1                   
Evidence: [HIPP]
Reference(s): [2] Lee CA., et al., 1983
Terminator(s)
Type: rho-independent
Sequence: gcctttcaaaAGTAAGCAACGTCTGCTTACTgcccctctac
Reference(s): [1] Figge RM., et al., 1994
Type: rho-independent
Sequence: cctctacctgCTTCGGCCGATAAAGCCGACGataatactcc
Reference(s): [1] Figge RM., et al., 1994
Type: rho-independent
Sequence: aatcgcgttaCGGGGAGGAAGTTTTTTCAGATACTCCCggaacgcctg
Reference(s): [1] Figge RM., et al., 1994
Type: rho-independent
Sequence: ccgtattaccCCGCGCCGGGAATGCGCGGccgccaattt
Reference(s): [1] Figge RM., et al., 1994
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote CRP-cAMP activator mtlAp 3771916 3771937 -261.5 acgaaggcatAACATGCTGTAGATCACATCAGgtgaacgccg nd [APIORCISFBSCS], [BPP] [2], [3], [4]
remote CRP-cAMP activator mtlAp 3771958 3771979 -219.5 taagaaaataTCTTGTGATTCAGATCACAAAGattcaacaaa nd [APIORCISFBSCS], [BPP] [2], [3], [4], [5]
remote CRP-cAMP activator mtlAp 3772002 3772023 -175.5 atcaaaacaaAAATGTGACACTACTCACATTTaaatgccatt nd [APIORCISFBSCS], [BPP] [2], [3], [4], [5]
remote CRP-cAMP activator mtlAp 3772075 3772096 -102.5 tacacaagcgTTTTGTGATGAACGTCACGTCAattacctctc nd [APIORCISFBSCS], [BPP] [2], [3], [4], [5]
proximal CRP-cAMP activator mtlAp 3772119 3772140 -58.5 ccccctatatTTATGTGATTGATATCACACAAaaggccgtcg nd [APIORCISFBSCS], [BPP] [2], [3], [4], [5]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal Cra repressor mtlAp 3772157 3772174 -22.5 gtcgactggaCAGTTAACCGATTCAGTGccagatttcg nd [BPP], [GEA] [6]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote Fis1 repressor mtlAp 3772317 3772331 137.0 gcaaagctttGGTCGTTTCCTCAGCaacatggtga nd [AIBSCS], [CV(GEA/ROMA)], [GEA] [7]
Note(s): 1Repression of mtl transcription occurs only when both MtlR and Fis are expressed.7Repression of mtl transcription occurs only when both MtlR and Fis are expressed.





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