RegulonDB RegulonDB 10.9: Operon Form
   

pncB operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: pncB
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: pncB
Synonym(s): OP00273
Gene(s): pncB   Genome Browser M3D Gene expression COLOMBOS
Reference(s): [1] Otsuka J., et al., 1996
[2] Wubbolts MG., et al., 1990
Promoter
Name: pncBp
+1: 990414
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 58
Sequence: aaaggtggcatatggtgtgatcggggttcaataaattgcgaaacaaggtatactccagcaGttcctgaagatgtttattgt
                           -35                   -10        +1                   
Evidence: [ICWHO]
[RS-EPT-CBR]
[TIM]
Reference(s): [3] Huerta AM., et al., 2003
[4] Salgado H, et al., 2012
[2] Wubbolts MG., et al., 1990
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal CRP-cAMP repressor pncBp 990441 990462 -37.5 aaggtggcatATGGTGTGATCGGGGTTCAATAAattgcgaaac nd [AIBSCS], [GEA] [5]
remote CRP-cAMP repressor pncBp 990611 990632 -207.5 tggcttgtggCTGTTGAGTCATAAATAACCTTTagcatctttt nd [AIBSCS], [GEA] [5]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal NadR repressor pncBp 990386 990403 20.5 gttcctgaagATGTTTATTGTACTAAACGctcctgtacg nd [AIBSCS] nd




Reference(s)    

 [1] Otsuka J., Watanabe H., Mori KT., 1996, Evolution of transcriptional regulation system through promiscuous coupling of regulatory proteins with operons; suggestion from protein sequence similarities in Escherichia coli., J Theor Biol 178(2):183-204

 [2] Wubbolts MG., Terpstra P., van Beilen JB., Kingma J., Meesters HA., Witholt B., 1990, Variation of cofactor levels in Escherichia coli. Sequence analysis and expression of the pncB gene encoding nicotinic acid phosphoribosyltransferase., J Biol Chem 265(29):17665-72

 [3] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [4] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [5] Zheng D., Constantinidou C., Hobman JL., Minchin SD., 2004, Identification of the CRP regulon using in vitro and in vivo transcriptional profiling., Nucleic Acids Res 32(19):5874-93


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