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Name: | manXYZ | ||||||||||
Synonym(s): | OP00275, ptsX | ||||||||||
Gene(s): | manX, manY, manZ Genome Browser M3D Gene expression COLOMBOS | ||||||||||
Note(s): | The plasticity of the promoter regions was demonstrated for two CRP target genes, ptsG and manX. Four genetically different strains with the crp gene deleted were propagated in a glucose minimal medium, the crp gene. This deletion led to deleterious effects on growth in the four strains, but most of the populations quickly evolved compensatory changes that restored fast growth and were specific to the glucose environment in which they evolved. The adaptation involved plasticity of the promoter regions of two CRP target genes involved in glucose transport, ptsG and manX Lamrabet O, Plumbridge J, Martin M, Lenski RE, Schneider D, Hindré T,2019 | ||||||||||
Reference(s): |
[1] Plumbridge J. 1998 [2] Plumbridge J., et al., 1991 [3] Plumbridge J., et al., 1995 |
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Promoter | |||||||||||
Name: | manXp | ||||||||||
+1: | 1901933 | ||||||||||
Sigma Factor: | Sigma70 Sigmulon | ||||||||||
Distance from start of the gene: | 115 | ||||||||||
Sequence: |
gaaagttaaattacggatcttcatcacataaaataattttttcgatatctaaaataaatcGcgaaacgcaggggtttttgg -35 -10 +1 |
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Note(s): | The homology with the consensus -35 sequences is poor, but it has been noted that the lack of a good -35 sequences is a characteristic of positively regulated genes Plumbridge J.,1998. | ||||||||||
Evidence: |
[COMP-AINF] [COMP-HINF-POSITIONAL-IDENTIFICATION] [EXP-IDA-TRANSCRIPTION-INIT-MAPPING] |
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Reference(s): |
[4] Huerta AM., et al., 2003 [1] Plumbridge J. 1998 |
Type | Transcription factor | Function | Promoter | Binding Sites | Growth Conditions | Evidence | Confidence level (C: Confirmed, S: Strong, W: Weak) | Reference(s) | |||
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LeftPos | RightPos | Central Rel-Pos | Sequence | ||||||||
proximal | CRP-cyclic-AMP | activator | manXp | 1901830 | 1901851 | -92.5 | ctttgcaaacGAATGTGACAAGGATATTTTACctttcgaaat | nd | [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [COMP-AINF-SIMILAR-TO-CONSENSUS], [COMP-HINF-SIMILAR-TO-CONSENSUS] | W | [2], [7] |
proximal | CRP-cyclic-AMP | activator | manXp | 1901882 | 1901903 | -40.5 | cgaaagttaaATTACGGATCTTCATCACATAAaataattttt | nd | [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [COMP-HINF-SIMILAR-TO-CONSENSUS], [EXP-IDA-BINDING-OF-CELLULAR-EXTRACTS] | S | [1], [2] |
Type | Transcription factor | Function | Promoter | Binding Sites | Growth Conditions | Evidence | Confidence level (C: Confirmed, S: Strong, W: Weak) | Reference(s) | |||
---|---|---|---|---|---|---|---|---|---|---|---|
LeftPos | RightPos | Central Rel-Pos | Sequence | ||||||||
remote | Cra | repressor | manXp | 1901965 | 1901981 | 40.5 | tgtagcccttATCTGAATCGATTCGATtgtggacgac | nd | [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [COMP-AINF-SIMILAR-TO-CONSENSUS] | W | [8] |
Type | Transcription factor | Function | Promoter | Binding Sites | Growth Conditions | Evidence | Confidence level (C: Confirmed, S: Strong, W: Weak) | Reference(s) | |||
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LeftPos | RightPos | Central Rel-Pos | Sequence | ||||||||
proximal | Mlc | repressor | manXp | 1901843 | 1901865 | -79.0 | tgtgacaaggATATTTTACCTTTCGAAATTTCTgctaatcgaa | nd | [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [EXP-IDA-BINDING-OF-CELLULAR-EXTRACTS] | W | [1] |
proximal | Mlc | repressor | manXp | 1901909 | 1901931 | -13.0 | cataaaataaTTTTTTCGATATCTAAAATAAATcgcgaaacgc | nd | [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [EXP-IDA-BINDING-OF-CELLULAR-EXTRACTS] | W | [1] |
Type | Transcription factor | Function | Promoter | Binding Sites | Growth Conditions | Evidence | Confidence level (C: Confirmed, S: Strong, W: Weak) | Reference(s) | |||
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LeftPos | RightPos | Central Rel-Pos | Sequence | ||||||||
proximal | NagC | repressor | manXp | 1901842 | 1901864 | -80.0 | atgtgacaagGATATTTTACCTTTCGAAATTTCtgctaatcga | nd | [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [COMP-AINF-SIMILAR-TO-CONSENSUS], [COMP-HINF-SIMILAR-TO-CONSENSUS], [EXP-IDA-BINDING-OF-CELLULAR-EXTRACTS] | S | [1], [2] |
proximal | NagC | repressor | manXp | 1901925 | 1901947 | 4.0 | cgatatctaaAATAAATCGCGAAACGCAGGGGTttttggttgt | nd | [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [EXP-IDA-BINDING-OF-CELLULAR-EXTRACTS] | W | [1], [2] |
sRNA | TU Regulated | Function | Binding Sites | Regulatory Mechanism | Evidence (Confirmed, Strong, Weak) | Reference(s) | ||
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PosLeft | PosRight | Target sequence (mRNA) | ||||||
small regulatory RNA SgrS | manXYZ | repressor | 1903038 | 1903052 | ACUCAGUUUUCACAC | TRANSLATION-BLOCKING, MRNA-DEGRADATION | [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [EXP-IMP-SITE-MUTATION], [EXP-IPI] | [5], [6] |
Reference(s) |
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