RegulonDB RegulonDB 10.8: Operon Form
   

wza-wzb-wzc-wcaAB operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: wza-wzb-wzc-wcaAB
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit       
Name: wzbc
Gene(s): wzc, wzb, wza   Genome Browser M3D Gene expression COLOMBOS
Note(s): The expression of this operon is increased when colicin (at 30 ng/ml) is added to the medium 23421615.
Evidence: [ITC] Inferred through co-regulation
Reference(s): [1] Rhodius VA., et al., 2005
Promoter
Name: wzap2
+1: 2137193
Sigma Factor: Sigma24 Sigmulon
Distance from start of the gene: -50
Sequence: taaataaacaatgatgaaatccaaaatgaaattgatgccattattggtgtcagtaaccttGataagcggttgcacagtact
Note(s): The wza gene may have been misannotated, because the transcription start site of wzap2 is 50 bp downstream of the start site of the gene Rhodius VA,2005
Evidence: [AIPP]
[TIM]
Reference(s): [1] Rhodius VA., et al., 2005


Transcription unit          
Name: wza-wzb-wzc-wcaAB
Synonym(s): OP00290, cps, cpsB, cpsGB
Gene(s): wcaB, wcaA, wzc, wzb, wza   Genome Browser M3D Gene expression COLOMBOS
Note(s): There is no real evidence that proves the cotranscription of these five genes, but they are closely coded in a small chromosome region.
Reference(s): [2] Ebel W., et al., 1999
[3] Stout V. 1996
Promoter
Name: wzap
+1: 2137583
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 340
Sequence: atagccaattaccgaattgttatcttgcctgctattccgttagctgtaacacttcctcctGcattattggaaagccaatat
                         -35                    -10         +1                   
Note(s): Although no obvious σ70 promoter region has been found, two good -10 region have been detected. This start site shows that the two best -10 regions are not used and the actual -35 and -10 regions of this operon may well be TTGCCT and AACACT. These sequences have four and three of six matches, respectively, to the consensus promoter sites.
Evidence: [HIPP]
[TIM]
Reference(s): [3] Stout V. 1996
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal RcsAB activator wzap 2137659 2137673 -83.0 taaaaaccatATTGAATGACACTTAatataattct nd [AIBSCS], [CV(GEA/ROMA)], [GEA] [2]
remote RcsAB2 activator wzap 2137681 2137695 -105.0 attgtcaaccTAAAGAAACTCCTAAaaaccatatt nd [BPP], [CV(CHIP-SV/SM)], [SM] [3], [4], [5]
Note(s): 2RcsB is apparently essential for cps expression. As a matter of fact, both RcsA and RcsB are required for optimal cps expression, since cps expression in an rcsB strain is low and cps expression cannot be activated by RcsA in the absence of RcsB, suggesting an auxiliary role in cps expression. But RcsA is not absolutely dependent on RcsB. There are no in vitro data that demonstrate RcsA binding to the cps promoter region. Thus, direct interaction of RcsB and/or RcsA in the E. coli cps promoter region remains to be shown. RcsA and RcsB are proposed to form heterodimers in order to be functional in the activation of cps. Multicopy RcsB can overcome the absence of RcsA to activate cps expression. Osmotic shock has been shown to be dependent on RcsA, RcsB, RcsC, and MdoH. Several global regulators bind and repress cps, such as CRP and H-NS.1RcsB is apparently essential for cps expression. As a matter of fact, both RcsA and RcsB are required for optimal cps expression, since cps expression in an rcsB strain is low and cps expression cannot be activated by RcsA in the absence of RcsB, suggesting an auxiliary role in cps expression. But RcsA is not absolutely dependent on RcsB. There are no in vitro data that demonstrate RcsA binding to the cps promoter region. Thus, direct interaction of RcsB and/or RcsA in the E. coli cps promoter region remains to be shown. RcsA and RcsB are proposed to form heterodimers in order to be functional in the activation of cps. Multicopy RcsB can overcome the absence of RcsA to activate cps expression. Osmotic shock has been shown to be dependent on RcsA, RcsB, RcsC, and MdoH. Several global regulators bind and repress cps, such as CRP and H-NS.


RNA cis-regulatory element    
Regulation, transcriptional elongation  
Attenuator type: Translational
Strand: reverse
  Structure type Energy LeftPos RightPos Sequence (RNA-strand)
  terminator -10.3 2133408 2133437 tcacgccgcaTCCGGCAAGCAAACCAGCTCATAAGCCGGgagaacaacc
Notes: "The provided "Sequence" is that of the RNA strand, i.e. U's are shown instead of T's and regulators on the reverse strand will appear as the reverse complement of the sequence delimited by LeftPos-RigtPos"
REGULATION, RNA cis-regulatory element:  
Rfam type: Cis-reg
Strand: reverse
  Description Rfam score Left Pos Right Pos Sequence (RNA-strand)
  JUMPstart RNA 57.3 2137422 2137460 uaaacuaaauCAGUGUAUUGGUAGCUAAAAAGCCAGGGGCGGUAGCGUGucuggaugcc
Notes: "The provied "Sequence" is that of the RNA strand, i.e. U's are shown instead of T's and regulators on the reverse strand will appears as the reverse complement of the sequence delimited by LeftPos-RightPos"





RegulonDB