RegulonDB RegulonDB 10.9: Operon Form
   

treBC operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: treBC
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: treBC
Synonym(s): OP00298
Gene(s): treC, treB   Genome Browser M3D Gene expression COLOMBOS
Note(s): The mdtEF-tolC, malEFG, gadBC, treBC, entCEBA-ybdB, hdeAB-yhiD, and alaE operons were differentially regulated after induction of the nonnative organic acid citramalate Webb J,2019
Evidence: [CV(LTED/PM)] cross validation(LTED/PM)
[IEP] Inferred from expression pattern
[ITCR] Inferred through co-regulation
[LTED] Length of transcript experimentally determined
[PM] Polar mutation
Reference(s): [1] Horlacher R., et al., 1997
[2] Klein W., et al., 1995
Promoter
Name: treBp
+1: 4466210
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 30
Sequence: tcgctgcgtttcgggaacgttcccgtttttaaatttttccgcgcaatatattctgcagccAaccaaaaatgtcatctgcca
                          -35                  -10          +1                   
Note(s): The activity of this promoter appears to be decreased in the presence of σE. It is repressed during the first 5 minutes following σE induction and decreased from 5 to 10 minutes and from 10 to 20 minutes after induction of the σ factor Lacoux C,2020.
Evidence: [HIPP]
[ICWHO]
[TIM]
Reference(s): [3] Huerta AM., et al., 2003
[2] Klein W., et al., 1995
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote ArcA-Phosphorylated activator treBp 4466440 4466454 -237.0 tggcgagcgtCGGTAATACGCCGTTAatgaaattcc nd [AIBSCS], [GEA] [4]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal CRP-cAMP1 repressor treBp 4466188 4466209 12.0 ttctgcagccAACCAAAAATGTCATCTGCCATGgggctttatg nd [APIORCISFBSCS] [2]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal CRP-cAMP1 unknown treBp 4466260 4466281 -60.5 cctgaacgatAAATTGTGATCTTCGCTGCGTTTcgggaacgtt nd [AIBSCS], [APIORCISFBSCS], [GEA] [2], [5]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal TreR repressor treBp 4466250 4466266 -48.0 gtgatcttcgCTGCGTTTCGGGAACGTTcccgttttta nd [APIORCISFBSCS], [BPP], [GEA] [1], [2]
proximal TreR repressor treBp 4466283 4466299 -81.0 accctgtcctGATCGTTTCCTGAACGATaaattgtgat nd [APIORCISFBSCS], [BPP], [GEA] [1], [2]
Note(s): 1The CRP function in the promoter for treB is not known, but the position of the binding site on this promoter suggests that CRP is a repressor of this promoter.1The CRP function in the promoter for treB is not known.3The CRP function in the promoter for treB is not known.
5The CRP function in the promoter for treB is not known, but the position of the binding site on this promoter suggests that CRP is a repressor of this promoter.


RNA cis-regulatory element    
Regulation, transcriptional elongation  
Attenuator type: Transcriptional
Strand: reverse
  Structure type Energy LeftPos RightPos Sequence (RNA-strand)
  terminator -6.1 4466232 4466259 cgctgcgtttCGGGAACGTTCCCGTTTTTAAATTTTTccgcgcaata
  anti-terminator -14.6 4466252 4466297 ctgtcctgatCGTTTCCTGAACGATAAATTGTGATCTTCGCTGCGTTTCGGGAACgttcccgttt
Notes: "The provided "Sequence" is that of the RNA strand, i.e. U's are shown instead of T's and regulators on the reverse strand will appear as the reverse complement of the sequence delimited by LeftPos-RigtPos"





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