RegulonDB RegulonDB 10.8: Operon Form
   

fadBA operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: fadBA
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: fadBA
Synonym(s): OP00303
Gene(s): fadA, fadB   Genome Browser M3D Gene expression COLOMBOS
Evidence: [PAGTSBP] Products of adjacent genes in the same biological process
Reference(s): [1] Yang SY. 1991
[2] Yang XY., et al., 1991
Promoter
Name: fadBp
+1: 4031013
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 42
Sequence: atcggcatttctttaatcttttgtttgcatatttttaacacaaaatacacacttcgactcAtctggtacgaccagatcacc
                         -35                    -10         +1                   
Evidence: [TIM]
Reference(s): [2] Yang XY., et al., 1991
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal ArcA-Phosphorylated repressor fadBp 4031035 4031049 -29.0 aatcttttgtTTGCATATTTTTAACacaaaataca nd [AIBSCS], [BPP], [CV(CHIP-SV/GEA/ROMA)], [CV(GEA/ROMA)], [GEA] [3], [4], [5]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal FadR repressor fadBp 4030997 4031013 9.0 acttcgactcATCTGGTACGACCAGATcaccttgcgg nd [APIORCISFBSCS], [BCE] [7], [8], [9]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal Fis activator fadBp 4031031 4031050 -27.0 taatcttttgTTTGCATATTTTTAACACAAaatacacact nd [AIBSCS], [CV(GEA/ROMA)], [GEA] [6]


RNA cis-regulatory element    
Regulation, transcriptional elongation  
Attenuator type: Transcriptional
Strand: reverse
  Structure type Energy LeftPos RightPos Sequence (RNA-strand)
  terminator -7.6 4031048 4031089 gcgtgatttcTGCCGAGCGTGATCAGATCGGCATTTCTTTAATCTTTTGTTtgcatatttt
  anti-terminator -9.4 4031083 4031129 gaccttaagtCTCCGCATCTTAGCACATCGTTCATCCAGAGCGTGATTTCTGCCGAgcgtgatcag
  anti-anti-terminator -4.4 4031124 4031179 tttttatagaGCGAGGCCAGTGATTCCATTTTTTACCCTTCTGTTTTTTTGACCTTAAGTCTCCGcatcttagca
Notes: "The provided "Sequence" is that of the RNA strand, i.e. U's are shown instead of T's and regulators on the reverse strand will appear as the reverse complement of the sequence delimited by LeftPos-RigtPos"




Reference(s)    

 [1] Yang SY., 1991, Location of the fadBA operon on the physical map of Escherichia coli., J Bacteriol 173(23):7405-6

 [2] Yang XY., Schulz H., Elzinga M., Yang SY., 1991, Nucleotide sequence of the promoter and fadB gene of the fadBA operon and primary structure of the multifunctional fatty acid oxidation protein from Escherichia coli., Biochemistry 30(27):6788-95

 [3] Cho BK., Knight EM., Palsson BO., 2006, Transcriptional regulation of the fad regulon genes of Escherichia coli by ArcA., Microbiology 152(Pt 8):2207-19

 [4] Liu X., De Wulf P., 2004, Probing the ArcA-P modulon of Escherichia coli by whole genome transcriptional analysis and sequence recognition profiling., J Biol Chem 279(13):12588-97

 [5] Spiro S., Guest JR., 1991, Adaptive responses to oxygen limitation in Escherichia coli., Trends Biochem Sci 16(8):310-4

 [6] Bradley MD., Beach MB., de Koning AP., Pratt TS., Osuna R., 2007, Effects of Fis on Escherichia coli gene expression during different growth stages., Microbiology 153(Pt 9):2922-40

 [7] DiRusso CC., Black PN., Weimar JD., 1999, Molecular inroads into the regulation and metabolism of fatty acids, lessons from bacteria., Prog Lipid Res 38(2):129-97

 [8] DiRusso CC., Heimert TL., Metzger AK., 1992, Characterization of FadR, a global transcriptional regulator of fatty acid metabolism in Escherichia coli. Interaction with the fadB promoter is prevented by long chain fatty acyl coenzyme A., J Biol Chem 267(12):8685-91

 [9] Gui L., Sunnarborg A., LaPorte DC., 1996, Regulated expression of a repressor protein: FadR activates iclR., J Bacteriol 178(15):4704-9


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