RegulonDB RegulonDB 10.9: Operon Form
   

sdaA operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: sdaA
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: sdaA
Synonym(s): OP00314
Gene(s): sdaA   Genome Browser M3D Gene expression COLOMBOS
Note(s): The sdaA gene codes for the l-serine deaminase, which participates in serine catabolism to pyruvate and ammonia Fraenkel YM,1995
Evidence: [BTEI] Boundaries of transcription experimentally identified
Reference(s): [1] Fraenkel YM., et al., 1995
[2] Su HS., et al., 1989
Promoter
Name: sdaAp
+1: 1896809
Sigma Factor: Sigma32 Sigmulon
Distance from start of the gene: 123
Sequence: ctatacttatctttacatctacaaaacactacttgagacaatcatcgcaatattagttaaAtcgcggtttttgattagttt
                            -35              -10            +1                   
Evidence: [HIPP]
[ICWHO]
[IDA]
[IEP]
[TIM]
Reference(s): [3] Huerta AM., et al., 2003
[4] Nonaka G., et al., 2006
[5] Wade JT., et al., 2006
Terminator(s)
Type: rho-independent
Sequence: tacttcttacTCGCCCATCTGCAACGGATGGGCGAatttataccc
Reference(s): [2] Su HS., et al., 1989
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd Lrp1 repressor sdaAp nd nd nd nd nd [RSE], [BPP], [GEA], [IGI] [1], [6]
Note(s): 1Additional evidence supports this TF-gene interaction with no precise position defined.
GCs_GeneExpression_EXP: lrp knockout mutant| MOPS| 0.2% glycerol| | 37.0 C| | | OD600 from 0.15 to 0.25| exponential phase| | |200 rpm. GCs_GeneExpression_CONTROL: wild-type| MOPS| 0.2% glycerol| | 37.0 C| | | OD600 from 0.15 to 0.25| exponential phase| | |200 rpm Kroner GM,2019br>GCs_GeneExpression_EXP: lrp knockout mutant| MOPS| 0.2% glycerol| | 37.0 C| | | OD600 from 1.8 to 2.2| transition point| | |200 rpm. GCs_GeneExpression_CONTROL: wild-type| MOPS| 0.2% glycerol| | 37.0 C| | | OD600 from 1.8 to 2.2| transition point| | |200 rpm Kroner GM,20191Additional evidence supports this TF-gene interaction with no precise position defined.
GCs_GeneExpression_EXP: lrp knockout mutant| MOPS| 0.2% glycerol| | 37.0 C| | | OD600 from 0.15 to 0.25| exponential phase| | |200 rpm. GCs_GeneExpression_CONTROL: wild-type| MOPS| 0.2% glycerol| | 37.0 C| | | OD600 from 0.15 to 0.25| exponential phase| | |200 rpm Kroner GM,2019br>GCs_GeneExpression_EXP: lrp knockout mutant| MOPS| 0.2% glycerol| | 37.0 C| | | OD600 from 1.8 to 2.2| transition point| | |200 rpm. GCs_GeneExpression_CONTROL: wild-type| MOPS| 0.2% glycerol| | 37.0 C| | | OD600 from 1.8 to 2.2| transition point| | |200 rpm Kroner GM,2019





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