RegulonDB RegulonDB 10.9: Operon Form

kbl-tdh operon and associated TUs in Escherichia coli K-12 genome

Name: kbl-tdh
This page displays every known transcription unit of this operon and their known regulation.

Transcription unit          
Name: kbl-tdh
Synonym(s): OP00316, tdh
Gene(s): tdh, kbl   Genome Browser M3D Gene expression COLOMBOS
Note(s): The kbl-tdh operon, coding for two enzymes of the threonine degradation pathway (threonine dehydrogenase and 2-amino-3-ketobutyrate lyase), is positively regulated by Lrp, dependent on leucine Rex JH,1991. Fraenkel YM,1995
A potential RNA G-quadruplex structure, formed by guanine-rich sequences located in the coding sequence region of the gene, was identified for tdh. This structure could regulate the expression of the gene, as observed for hemL gene expression 31964733.
Reference(s): [1] Landgraf JR., et al., 1994
Name: kblp
+1: 3792577
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 26
Sequence: tttgcagaagtgttaacgcgttatctcgtcgcgacctataagtttgggtaatatgtgctgGaatttgccctgtctggagaa
                       -35                       -10        +1                   
Evidence: [ICWHO]
Reference(s): [2] Aronson BD., et al., 1988
[3] Huerta AM., et al., 2003
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal Lrp1 activator kblp 3792636 3792650 -66.5 agttacacgtTATTTATCCTGAATTTtgcagaagtg nd [AIBSCS], [APIORCISFBSCS], [BPP], [GEA] [4]
proximal Lrp activator kblp 3792647 3792661 -77.0 gagagacatcAAGTTACACGTTATTTatcctgaatt nd [AIBSCS] nd
Note(s): 1Lrp regulates the tdh-kbl operon in a leucine-dependent manner Rex JH,1991.
2Lrp regulates the tdh-kbl operon in a leucine-dependent manner Rex JH,1991.

RNA cis-regulatory element    
Regulation, transcriptional elongation  
Attenuator type: Transcriptional
Strand: reverse
  Structure type Energy LeftPos RightPos Sequence (RNA-strand)
  terminator -16.4 3792745 3792782 caactgagatAGGAAAAATGATGCTTTGGCATCATTTTTTCTTTTTAtatcaatgca
Notes: "The provided "Sequence" is that of the RNA strand, i.e. U's are shown instead of T's and regulators on the reverse strand will appear as the reverse complement of the sequence delimited by LeftPos-RigtPos"