RegulonDB RegulonDB 11.1: Operon Form

chaBC operon and associated TUs in Escherichia coli K-12 genome

Name: chaBC
This page displays every known transcription unit of this operon and their known regulation.

Transcription unit          
Name: chaC
Gene(s): chaC   Genome Browser M3D Gene expression COLOMBOS
Evidence: [EXP-IDA-BOUNDARIES-DEFINED] Boundaries of transcription experimentally identified
[IC] Inferred by curator
Reference(s): [1] Reitzer L., et al., 2001
[2] Zaslaver A., et al., 2006
Name: chaCp
+1: 1272422
Sigma Factor: Sigma54 Sigmulon
Distance from start of the gene: 85
Sequence: agttaaagctattcgtgcggtgttgccttgcaagtggtccgtggattgcatattgtcccgTtagtggtttcaaaatgagca
                                   -24         -12          +1                   
Note(s): We assigned a putative transcription start site to this promoter based on the observation that the majority of the σ54-dependent promoters, whose transcription start sites were determined experimentally, present a distance of 11 nucleotides between the transcription start site and the -12 box Barrios H,1999.
Zaslaver et al. demonstrated in 2006, by means of a library of fluorescent transcription fusions, that this promoter can be transcribed in vitro Zaslaver A,2006. Based on this, a putative promoter was suggested, but the +1 site of the transcription initiation has not been determined, although there exists promoter activity.
Evidence: [COMP-AINF]
Reference(s): [3] Huerta AM., et al., 2003
[1] Reitzer L., et al., 2001
[2] Zaslaver A., et al., 2006
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence Confidence level (C: Confirmed, S: Strong, W: Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd Rob activator chaCp nd nd nd nd nd [EXP-IEP-GENE-EXPRESSION-ANALYSIS] W [4]

Transcription unit