RegulonDB RegulonDB 10.9: Operon Form
   

aroH operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: aroH
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: aroH
Synonym(s): OP00012
Gene(s): aroH   Genome Browser M3D Gene expression COLOMBOS
Note(s): There is a putative CRP-binding site between the aroHp1 and the aroHp2 promoters, but this protein did not demonstrate any effect on the promoters Hudson GS,1991
The trpLEDCBA, aroH, and trpR operons are coordinately regulated by the TrpR transcriptional repressor in response to intracellular levels of L-tryptophan (Kumamoto AA,1987and references therein). TrpR acts differently at each operator and binds to multiple helically stacked sites Kumamoto AA,1987
Evidence: [BTEI] Boundaries of transcription experimentally identified
Reference(s): [1] Hudson GS., et al., 1991
Promoter
Name: aroHp1
+1: 1788318
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 117
Sequence: cttagtcgccgaatgtactagagaactagtgcattagcttatttttttgttatcatgctaAccacccggcgaggtgtgaca
                             -35                   -10      +1                   
Note(s): The aroHp1 promoter is active in minimal medium Hudson GS,1991
aroHp1 is regulated over a four- to fivefold range by TrpR Kumamoto AA,1987and references therein|.
Evidence: [HIPP]
[ICWHO]
[TIM]
Reference(s): [1] Hudson GS., et al., 1991
[2] Huerta AM., et al., 2003
Terminator(s)
Type: rho-independent
Sequence: tctgaatgcgTGCCCATTCCTGACGGAATGGGCAtttctgcgca
Reference(s): [1] Hudson GS., et al., 1991
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal rpR-L-tryptophan repressor aroHp1 1788272 1788289 -37.5 gtcgccgaatGTACTAGAGAACTAGTGCAttagcttatt nd [APIORCISFBSCS], [BPP], [GEA] [3], [4], [5], [6], [7]
proximal rpR-L-tryptophan repressor aroHp1 1788280 1788297 -29.5 atgtactagaGAACTAGTGCATTAGCTTAtttttttgtt nd [APIORCISFBSCS], [BPP], [GEA] [3], [4], [5], [6], [7]


Transcription unit       
Name: aroH
Gene(s): aroH   Genome Browser M3D Gene expression COLOMBOS
Evidence: [BTEI] Boundaries of transcription experimentally identified
Reference(s): [1] Hudson GS., et al., 1991
Promoter
Name: aroHp2
+1: 1788384
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 51
Sequence: cggcgaggtgtgacacacctcgcacttgaaatcagcagcgattggtttatcgtgatgcgcAtcacttcccggcagtcctgc
                          -35                   -10         +1                   
Note(s): The aroHp2 promoter is active in rich medium with aromatic amino acids by an unknown mechanism Hudson GS,1991
Evidence: [ICWHO]
[RS-EPT-CBR]
[TIM]
Reference(s): [1] Hudson GS., et al., 1991
[2] Huerta AM., et al., 2003
[8] Salgado H, et al., 2012
Terminator(s)
Type: rho-independent
Sequence: tctgaatgcgTGCCCATTCCTGACGGAATGGGCAtttctgcgca
Reference(s): [1] Hudson GS., et al., 1991


RNA cis-regulatory element    
Regulation, transcriptional elongation  
Attenuator type: Transcriptional
Strand: forward
Evidence: [ICA] Inferred by computational analysis
Reference(s): [9] Merino E, et al., 2005
  Structure type Energy LeftPos RightPos Sequence (RNA-strand)
  terminator -11.8 1788265 1788308 gccttagtcgCCGAATGTACTAGAGAACTAGTGCATTAGCTTATTTTTTTGTTatcatgctaa
  anti-terminator -7.1 1788243 1788280 accaagatccTCGATATCATGGGCCTTAGTCGCCGAATGTACTAGAGaactagtgca
  anti-anti-terminator -6.5 1788204 1788259 tggattaacaGTACCAATTATTCGGTAGAAGAGATTGCCACCAAGATCCTCGATATCATGGGCCTtagtcgccga
Notes: "The provided "Sequence" is that of the RNA strand, i.e. U's are shown instead of T's and regulators on the reverse strand will appear as the reverse complement of the sequence delimited by LeftPos-RigtPos"




Reference(s)    

 [1] Hudson GS., Rellos P., Davidson BE., 1991, Two promoters control the aroH gene of Escherichia coli., Gene 102(1):87-91

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [3] Grove CL., Gunsalus RP., 1987, Regulation of the aroH operon of Escherichia coli by the tryptophan repressor., J Bacteriol 169(5):2158-64

 [4] Jeeves M., Evans PD., Parslow RA., Jaseja M., Hyde EI., 1999, Studies of the Escherichia coli Trp repressor binding to its five operators and to variant operator sequences., Eur J Biochem 265(3):919-28

 [5] Klig LS., Carey J., Yanofsky C., 1988, trp repressor interactions with the trp aroH and trpR operators. Comparison of repressor binding in vitro and repression in vivo., J Mol Biol 202(4):769-77

 [6] Kumamoto AA., Miller WG., Gunsalus RP., 1987, Escherichia coli tryptophan repressor binds multiple sites within the aroH and trp operators., Genes Dev 1(6):556-64

 [7] Yang J., Gunasekera A., Lavoie TA., Jin L., Lewis DE., Carey J., 1996, In vivo and in vitro studies of TrpR-DNA interactions., J Mol Biol 258(1):37-52

 [8] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [9] Merino E, Yanofsky C., 2005, Transcription attenuation: a highly conserved regulatory strategy used by bacteria., Trends Genet. 2005 May;21(5):260-4.


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