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| Operon |  |
|---|---|
| Name: | aroH |
| This page displays every known transcription unit of this operon and their known regulation. | |
| Name: | aroH | |||||||||
| Synonym(s): | OP00012 | |||||||||
| Gene(s): | aroH Genome Browser M3D Gene expression COLOMBOS | |||||||||
| Note(s): | There is a putative CRP-binding site between the aroHp1 and the aroHp2 promoters, but this protein did not demonstrate any effect on the promoters Hudson GS,1991 The trpLEDCBA, aroH, and trpR operons are coordinately regulated by the TrpR transcriptional repressor in response to intracellular levels of L-tryptophan (Kumamoto AA,1987and references therein). TrpR acts differently at each operator and binds to multiple helically stacked sites Kumamoto AA,1987 The expression of the gene aroH is enhanced under oxygen limitation Kawaguchi H, Miyagawa H, Nakamura-Tsuruta S, Takaya N, Ogino C, Kondo A,2019. |
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| Evidence: | [BTEI] Boundaries of transcription experimentally identified | |||||||||
| Reference(s): | [1] Hudson GS., et al., 1991 | |||||||||
| Promoter | ||||||||||
| Name: | aroHp1 | |||||||||
| +1: | 1788318 | |||||||||
| Sigma Factor: | Sigma70 Sigmulon | |||||||||
| Distance from start of the gene: | 117 | |||||||||
| Sequence: |
cttagtcgccgaatgtactagagaactagtgcattagcttatttttttgttatcatgctaAccacccggcgaggtgtgaca -35 -10 +1 |
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| Note(s): | The aroHp1 promoter is active in minimal medium Hudson GS,1991 aroHp1 is regulated over a four- to fivefold range by TrpR Kumamoto AA,1987and references therein|. |
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| Evidence: |
[HIPP] [ICWHO] [TIM] |
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| Reference(s): |
[1] Hudson GS., et al., 1991 [2] Huerta AM., et al., 2003 |
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| Terminator(s) | ||||||||||
| Type: | rho-independent | |||||||||
| Sequence: | tctgaatgcgTGCCCATTCCTGACGGAATGGGCAtttctgcgca | |||||||||
| Reference(s): | [1] Hudson GS., et al., 1991 | |||||||||
| Type | Transcription factor | Function | Promoter | Binding Sites | Growth Conditions | Evidence (Confirmed, Strong, Weak) | Reference(s) | |||
|---|---|---|---|---|---|---|---|---|---|---|
| LeftPos | RightPos | Central Rel-Pos | Sequence | |||||||
| proximal | TrpR-L-tryptophan | repressor | aroHp1 | 1788272 | 1788289 | -37.5 | gtcgccgaatGTACTAGAGAACTAGTGCattagcttat | nd | [GEA], [APIORCISFBSCS], [BPP] | [3], [4], [5], [6], [7] |
| proximal | TrpR-L-tryptophan | repressor | aroHp1 | 1788280 | 1788297 | -29.5 | atgtactagaGAACTAGTGCATTAGCTTatttttttgt | nd | [GEA], [APIORCISFBSCS], [BPP] | [3], [4], [5], [6], [7] |
| Name: | aroH |
| Gene(s): | aroH Genome Browser M3D Gene expression COLOMBOS |
| Note(s): | The expression of the gene aroH is enhanced under oxygen limitation Kawaguchi H, Miyagawa H, Nakamura-Tsuruta S, Takaya N, Ogino C, Kondo A,2019. |
| Evidence: | [BTEI] Boundaries of transcription experimentally identified |
| Reference(s): | [1] Hudson GS., et al., 1991 |
| Promoter | |
| Name: | aroHp2 |
| +1: | 1788384 |
| Sigma Factor: | Sigma70 Sigmulon |
| Distance from start of the gene: | 51 |
| Sequence: |
cggcgaggtgtgacacacctcgcacttgaaatcagcagcgattggtttatcgtgatgcgcAtcacttcccggcagtcctgc -35 -10 +1 |
| Note(s): | The aroHp2 promoter is active in rich medium with aromatic amino acids by an unknown mechanism Hudson GS,1991 |
| Evidence: |
[ICWHO] [RS-EPT-CBR] [TIM] |
| Reference(s): |
[1] Hudson GS., et al., 1991 [2] Huerta AM., et al., 2003 [8] Salgado H, et al., 2012 |
| Terminator(s) | |
| Type: | rho-independent |
| Sequence: | tctgaatgcgTGCCCATTCCTGACGGAATGGGCAtttctgcgca |
| Reference(s): | [1] Hudson GS., et al., 1991 |
| RNA cis-regulatory element | |
|---|
| Regulation, transcriptional elongation | |
| Attenuator type: | Transcriptional |
| Strand: | forward |
| Evidence: | [ICA] Inferred by computational analysis |
| Reference(s): | [9] Merino E, et al., 2005 |
| Structure type | Energy | LeftPos | RightPos | Sequence (RNA-strand) | |
|---|---|---|---|---|---|
| terminator | -11.8 | 1788265 | 1788308 | gccttagtcgCCGAATGTACTAGAGAACTAGTGCATTAGCTTATTTTTTTGTTatcatgctaa | |
| anti-terminator | -7.1 | 1788243 | 1788280 | accaagatccTCGATATCATGGGCCTTAGTCGCCGAATGTACTAGAGaactagtgca | |
| anti-anti-terminator | -6.5 | 1788204 | 1788259 | tggattaacaGTACCAATTATTCGGTAGAAGAGATTGCCACCAAGATCCTCGATATCATGGGCCTtagtcgccga |
| Notes: "The provided "Sequence" is that of the RNA strand, i.e. U's are shown instead of T's and regulators on the reverse strand will appear as the reverse complement of the sequence delimited by LeftPos-RigtPos" |
| Reference(s) |
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|---|---|
[HIPP] Human inference of promoter position