RegulonDB RegulonDB 11.1: Operon Form
   

bioA operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: bioA
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: bioA
Synonym(s): OP00015
Gene(s): bioA   Genome Browser M3D Gene expression COLOMBOS
Reference(s): [1] Nath SK., et al., 1982
Promoter
Name: bioAp
+1: 809292
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 35
Sequence: gtgagccatggggcttctccaaaacgtgttttttgttgttaattcggtgtagacttgtaaAcctaaatcttttcaatttgg
                                  -35                  -10  +1                   
Evidence: [COMP-AINF]
[COMP-HINF-POSITIONAL-IDENTIFICATION]
Reference(s): [2] Huerta AM., et al., 2003
[3] Otsuka A., et al., 1978
TF binding sites (TFBSs)
[BirA,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence Confidence level (C: Confirmed, S: Strong, W: Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal BirA-biotinyl-5'-adenylate repressor bioAp 809262 809302 11.5 aattcggtgtAGACTTGTAAACCTAAATCTTTTCAATTTGGTTTACAAGTCgattatgaca nd [EXP-IMP-SITE-MUTATION] nd nd


RNA cis-regulatory element    
Regulation, transcriptional elongation  
Attenuator type: Translational
Strand: reverse
  Structure type Energy LeftPos RightPos Sequence (RNA-strand)
  terminator -16.0 809262 809301 attcggtgtaGACTTGTAAACCTAAATCTTTTCAATTTGGTTTACAAGTcgattatgac
Notes: "The provided "Sequence" is that of the RNA strand, i.e. U's are shown instead of T's and regulators on the reverse strand will appear as the reverse complement of the sequence delimited by LeftPos-RigtPos"


Evidence    

 [COMP-AINF] Inferred computationally without human oversight

 [COMP-HINF-POSITIONAL-IDENTIFICATION] Human inference of promoter position

 [EXP-IMP-SITE-MUTATION] Site mutation


Reference(s)    

 [1] Nath SK., Guha A., 1982, Abortive termination of bioBFCD RNA synthesized in vitro from the bioABFCD operon of Escherichia coli K-12., Proc Natl Acad Sci U S A 79(6):1786-90

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [3] Otsuka A., Abelson J., 1978, The regulatory region of the biotin operon in Escherichia coli., Nature 276(5689):689-94

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