RegulonDB RegulonDB 10.10: Operon Form
   

metH operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: metH
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit       
Name: metH
Gene(s): metH   Genome Browser M3D Gene expression COLOMBOS
Evidence: [LTED] Length of transcript experimentally determined
Reference(s): [1] Marconi R., et al., 1991
Promoter
Name: metHp2
+1: 4223505
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 323
Sequence: ggtgggtcgtggaattgggtaacccggcttgttgcgccagttccgtgagtgccacactgcCattggattcggcaatccact
                             -35                  -10       +1                   
Evidence: [IHBCE]
[TIM]
Reference(s): [1] Marconi R., et al., 1991


Transcription unit          
Name: metH
Synonym(s): OP00055
Gene(s): metH   Genome Browser M3D Gene expression COLOMBOS
Reference(s): [2] Wehmeier UF., et al., 1992
Promoter
Name: metHp1
+1: 4223783
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 45
Sequence: acaaatctcatgttgcgtggtggtcgcttttaccacagatgcgtttatgccagtatggttTgttgaatttttattaaatct
                              -35                     -10   +1                   
Evidence: [HIPP]
[ICWHO]
Reference(s): [3] Huerta AM., et al., 2003
[4] Old IG., et al., 1990
TF binding sites (TFBSs)
[Fur,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote Fur-Fe2+ repressor metHp1 4223650 4223668 -124.0 ttttctgtatCGTGGAAATCATTTTCATTtttattgtta nd [AIBSCS] [5]
[MetR,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal MetR activator metHp1 4223719 4223733 -57.0 tgagttaatgTTGAACAAATCTCATgttgcgtggt nd [GEA], [APIORCISFBSCS] [4], [6], [7], [8]


Evidence    

 [IHBCE] Inferred by a human based on computational evidence

 [TIM] Transcription initiation mapping

 [HIPP] Human inference of promoter position

 [ICWHO] Inferred computationally without human oversight

 [AIBSCS] Automated inference based on similarity to consensus sequences

 [GEA] Gene expression analysis

 [APIORCISFBSCS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.


Reference(s)    

 [1] Marconi R., Wigboldus J., Weissbach H., Brot N., 1991, Transcriptional start and MetR binding sites on the Escherichia coli metH gene., Biochem Biophys Res Commun 175(3):1057-63

 [2] Wehmeier UF., Nobelmann B., Lengeler JW., 1992, Cloning of the Escherichia coli sor genes for L-sorbose transport and metabolism and physical mapping of the genes near metH and iclR., J Bacteriol 174(23):7784-90

 [3] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [4] Old IG., Margarita D., Glass RE., Saint Girons I., 1990, Nucleotide sequence of the metH gene of Escherichia coli K-12 and comparison with that of Salmonella typhimurium LT2., Gene 87(1):15-21

 [5] Stojiljkovic I., Baumler AJ., Hantke K., 1994, Fur regulon in gram-negative bacteria. Identification and characterization of new iron-regulated Escherichia coli genes by a fur titration assay., J Mol Biol 236(2):531-45

 [6] Cai XY., Maxon ME., Redfield B., Glass R., Brot N., Weissbach H., 1989, Methionine synthesis in Escherichia coli: effect of the MetR protein on metE and metH expression., Proc Natl Acad Sci U S A 86(12):4407-11

 [7] Cai XY., Redfield B., Maxon M., Weissbach H., Brot N., 1989, The effect of homocysteine on MetR regulation of metE, metR and metH expression in vitro., Biochem Biophys Res Commun 163(1):79-83

 [8] Jafri S., Urbanowski ML., Stauffer GV., 1995, A mutation in the rpoA gene encoding the alpha subunit of RNA polymerase that affects metE-metR transcription in Escherichia coli., J Bacteriol 177(3):524-9

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