RegulonDB RegulonDB 11.1: Operon Form

phoA-psiF operon and associated TUs in Escherichia coli K-12 genome

Name: phoA-psiF
This page displays every known transcription unit of this operon and their known regulation.

Transcription unit          
Name: phoA-psiF
Synonym(s): OP00071, phoA
Gene(s): phoA, psiF   Genome Browser M3D Gene expression COLOMBOS
Note(s): The expression of the gene phoA is increased under phosphate starvation Ulu?eker C, Torres-Bacete J, García JL, Hanczyc MM, Nogales J, Kahramano?ullar? O,2019 and acidic growth conditions in aerobiosis but not in microaerobiosis Marzan LW,2013 The increased expression appears to be caused by the transcription factor PhoB Marzan LW,2013
Reference(s): [1] Wanner BL., et al., 1981
[2] Yamada M., et al., 1989
Name: phoAp
+1: 401707
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 40
Sequence: aaagttaatcttttcaacagctgtcataaagttgtcacggccgagacttatagtcgctttGtttttattttttaatgtatt
                          -35                    -10        +1                   
Evidence: [AS-NAS]
Reference(s): [3] Huerta AM., et al., 2003
[4] Makino K., et al., 1986
[5] Shuttleworth H., et al., 1986
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence Confidence level (C: Confirmed, S: Strong, W: Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal PhoB-phosphorylated activator phoAp 401667 401685 -31.0 ttttcaacagCTGTCATAAAGTTGTCACGgccgagactt nd [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [EXP-IDA-BINDING-OF-CELLULAR-EXTRACTS], [EXP-IMP-SITE-MUTATION] W [6]

Transcription unit       
Name: psiF
Gene(s): psiF   Genome Browser M3D Gene expression COLOMBOS
Evidence: [EXP-IEP] Inferred from expression pattern
Name: psiFp
+1: 403231
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 50
Sequence: ccggcagtgaattttcgctgccgggtggtttttttgctgttagcaaccagacttaatggcAgatcacgggcgcatacgctc
                         -35                        -10     +1                   
Evidence: [COMP-AINF]

RNA cis-regulatory element    
Regulation, transcriptional elongation  
Attenuator type: Transcriptional
Strand: forward
  Structure type Energy LeftPos RightPos Sequence (RNA-strand)
  terminator -26.0 403163 403206 ctgaaataaaACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTGctgttagcaa
  anti-terminator -13.0 403139 403184 tacaccatgaAAGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTtcgctgccgg
Notes: "The provided "Sequence" is that of the RNA strand, i.e. U's are shown instead of T's and regulators on the reverse strand will appear as the reverse complement of the sequence delimited by LeftPos-RigtPos"