RegulonDB RegulonDB 10.8: Operon Form
   

cysJIH operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: cysJIH
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: cysJIH
Synonym(s): OP00106, cysJHI
Gene(s): cysH, cysI, cysJ   Genome Browser M3D Gene expression COLOMBOS
Note(s): cysJIH expression is inhibited by oleanolic and ursolic acid, which in turn affect the expression of the cysteine regulon and the stress response Grudniak AM,2011
Reference(s): [1] Ostrowski J., et al., 1989
Promoter
Name: cysJp
+1: 2891964
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 66
Sequence: ggttattagttatcgctatcccgtctttaatccacaccgtttgccccgttaaccttacctTctcttctgttttatgggcgc
                          -35                     -10       +1                   
Evidence: [TIM]
Reference(s): [2] Ostrowski J., et al., 1989
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal CysB-O-acetyl-L-serine activator cysJp 2891998 2892039 -55.0 attgctaaaaCAGGTTAGTCGATTTGGTTATTAGTTATCGCTATCCCGTCTTtaatccacac nd [APIORCISFBSCS] [4]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote IHF1 activator cysJp 2892082 2892094 -124.0 gctaaattcaTTTGTTTTTCATTaggttggtta nd [BPP], [GEA] [3]
Note(s): 1Sirko A,1998 demonstrated that IHF was necessary for full expression of the positively regulated cysJp promoter during stationary growth. In addition, they mentioned that IHF could act as a positive accessory factor, harnessing the effect of CysB under the transcription of cysJIH.
1Sirko A,1998 demonstrated that IHF was necessary for full expression of the positively regulated cysJp promoter during stationary growth. In addition, they mentioned that IHF could act as a positive accessory factor, harnessing the effect of CysB under the transcription of cysJIH.



Transcription unit          
Name: cysH
Gene(s): cysH   Genome Browser M3D Gene expression COLOMBOS
Promoter
Name: cysHp
+1: Unknown
Evidence: [IEP]
Reference(s): [5] Zaslaver A., et al., 2006
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd HypT-Met-oxidized activator cysHp nd nd nd nd nd [GEA] [6], [7]




Reference(s)    

 [1] Ostrowski J., Wu JY., Rueger DC., Miller BE., Siegel LM., Kredich NM., 1989, Characterization of the cysJIH regions of Salmonella typhimurium and Escherichia coli B. DNA sequences of cysI and cysH and a model for the siroheme-Fe4S4 active center of sulfite reductase hemoprotein based on amino acid homology with spinach nitrite reductase., J Biol Chem 264(26):15726-37

 [2] Ostrowski J., Kredich NM., 1989, Molecular characterization of the cysJIH promoters of Salmonella typhimurium and Escherichia coli: regulation by cysB protein and N-acetyl-L-serine., J Bacteriol 171(1):130-40

 [3] Sirko A., Weglenska A., Hulanicka D., 1998, Integration host factor positively regulates cycJIH transcription., Mol Gen Genet 258(1-2):174-7

 [4] Monroe RS., Ostrowski J., Hryniewicz MM., Kredich NM., 1990, In vitro interactions of CysB protein with the cysK and cysJIH promoter regions of Salmonella typhimurium., J Bacteriol 172(12):6919-29

 [5] Zaslaver A., Bren A., Ronen M., Itzkovitz S., Kikoin I., Shavit S., Liebermeister W., Surette MG., Alon U., 2006, A comprehensive library of fluorescent transcriptional reporters for Escherichia coli., Nat Methods 3(8):623-8

 [6] Drazic A., Miura H., Peschek J., Le Y., Bach NC., Kriehuber T., Winter J., 2013, Methionine oxidation activates a transcription factor in response to oxidative stress., Proc Natl Acad Sci U S A 110(23):9493-8

 [7] Gebendorfer KM., Drazic A., Le Y., Gundlach J., Bepperling A., Kastenmuller A., Ganzinger KA., Braun N., Franzmann TM., Winter J., 2012, Identification of a Hypochlorite-specific Transcription Factor from Escherichia coli., J Biol Chem 287(9):6892-903


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