RegulonDB

ilvIH operon and associated TUs in Escherichia coli K-12 genome

Operon
Name:	ilvIH
This page displays every known transcription unit of this operon and their known regulation.

Transcription unit

Name:	ilvIH
Gene(s):	ilvI, ilvH Genome Browser M3D Gene expression COLOMBOS
Evidence:	[EXP-IDA-TRANSCRIPT-LEN-DETERMINATION] Length of transcript experimentally determined
Reference(s):	[1] Blattner FR., et al., 1997 [2] Lago CT., et al., 1985 [3] Squires CH., et al., 1983
Promoter
Name:	ilvIp4
+1:	85420
Sigma Factor:	Sigma70 Sigmulon
Distance from start of the gene:	210
Sequence:	ttttattgaatgtagaattttattctgaatgtgtgggctctctattttaggattaattaaAaaaatagagaaattgctgta -35 -10 +1
Evidence:	[COMP-HINF-POSITIONAL-IDENTIFICATION] [EXP-IDA-TRANSCRIPTION-INIT-MAPPING]
Reference(s):	[4] Haughn GW., et al., 1985

Transcription unit

Name:	ilvIH
Gene(s):	ilvI, ilvH Genome Browser M3D Gene expression COLOMBOS
Evidence:	[EXP-IDA-TRANSCRIPT-LEN-DETERMINATION] Length of transcript experimentally determined
Reference(s):	[1] Blattner FR., et al., 1997 [2] Lago CT., et al., 1985 [3] Squires CH., et al., 1983
Promoter
Name:	ilvIp3
+1:	85453
Sigma Factor:	Sigma70 Sigmulon
Distance from start of the gene:	177
Sequence:	tgggctctctattttaggattaattaaaaaaatagagaaattgctgtaagttgtgggattCagccgatttattatcaattt -35 -10 +1
Evidence:	[COMP-HINF-POSITIONAL-IDENTIFICATION] [EXP-IDA-TRANSCRIPTION-INIT-MAPPING]
Reference(s):	[4] Haughn GW., et al., 1985

Transcription unit

Name:	ilvIH
Gene(s):	ilvI, ilvH Genome Browser M3D Gene expression COLOMBOS
Note(s):	The highest affinity of the interaction of Lrp upstream of ilvI has been observed at pH 8.0, although the cooperativity increases as the pH declines Ishii Y,2021.
Evidence:	[EXP-IDA-TRANSCRIPT-LEN-DETERMINATION] Length of transcript experimentally determined
Reference(s):	[1] Blattner FR., et al., 1997 [2] Lago CT., et al., 1985 [3] Squires CH., et al., 1983
Promoter
Name:	ilvIp2
+1:	85540
Distance from start of the gene:	90
Sequence:
Evidence:	[COMP-HINF-POSITIONAL-IDENTIFICATION] [EXP-IDA-TRANSCRIPTION-INIT-MAPPING] [EXP-IMP]
Reference(s):	[4] Haughn GW., et al., 1985 [5] Willins DA., et al., 1992

TF binding sites (TFBSs)

[Lrp,-] Phrase

Type	Transcription factor	Function	Promoter	Binding Sites				Growth Conditions	Evidence	Confidence level (C: Confirmed, S: Strong, W: Weak)	Reference(s)
Type	Transcription factor	Function	Promoter	LeftPos	RightPos	Central Rel-Pos	Sequence	Growth Conditions	Evidence		Reference(s)
remote	Lrp	repressor	ilvIp2	85341	85355	-192.0	agacatgaatGTCTGGTTTATTCTGcattttttat	nd	[COMP-HINF-SIMILAR-TO-CONSENSUS], [EXP-IDA-BINDING-OF-CELLULAR-EXTRACTS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS], [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [EXP-IMP-SITE-MUTATION], [COMP-HINF-SIMILAR-TO-CONSENSUS], [EXP-CHIP-SEQ], [EXP-IDA-BINDING-OF-CELLULAR-EXTRACTS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS], [EXP-IMP-SITE-MUTATION]	C	[4], [5], [6], [7], [8], [9], [10], [11], [12], [13]
remote	Lrp	repressor	ilvIp2	85372	85386	-161.0	ttattgaatgTAGAATTTTATTCTGaatgtgtggg	nd	[COMP-HINF-SIMILAR-TO-CONSENSUS], [EXP-IDA-BINDING-OF-CELLULAR-EXTRACTS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS], [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [EXP-IEP-MICROARRAY], [EXP-IMP-SITE-MUTATION], [COMP-AINF-PATTERN-DISCOVERY], [COMP-HINF-SIMILAR-TO-CONSENSUS], [EXP-CHIP-CHIP], [EXP-CHIP-SEQ], [EXP-IDA-BINDING-OF-CELLULAR-EXTRACTS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS], [EXP-IMP-SITE-MUTATION]	C	[4], [5], [6], [7], [8], [9], [10], [11], [12], [13], [14]
proximal	Lrp	repressor	ilvIp2	85453	85467	-80.0	ttgtgggattCAGCCGATTTATTATcaatttaatc	nd	[COMP-HINF-SIMILAR-TO-CONSENSUS], [EXP-IDA-BINDING-OF-CELLULAR-EXTRACTS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS], [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [EXP-IMP-SITE-MUTATION], [COMP-HINF-SIMILAR-TO-CONSENSUS], [EXP-CHIP-SEQ], [EXP-IDA-BINDING-OF-CELLULAR-EXTRACTS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS], [EXP-IMP-SITE-MUTATION]	C	[4], [5], [6], [7], [8], [9], [10], [11], [12], [13]
proximal	Lrp	repressor	ilvIp2	85488	85502	-45.0	ctctgtaatgGAGGATTTTATCGTTtcttttcacc	nd	[COMP-HINF-SIMILAR-TO-CONSENSUS], [EXP-IDA-BINDING-OF-CELLULAR-EXTRACTS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS], [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [EXP-IMP-SITE-MUTATION], [COMP-HINF-SIMILAR-TO-CONSENSUS], [EXP-CHIP-SEQ], [EXP-IDA-BINDING-OF-CELLULAR-EXTRACTS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS], [EXP-IMP-SITE-MUTATION]	C	[4], [5], [6], [7], [8], [9], [10], [11], [12], [13]
proximal	Lrp	repressor	ilvIp2	85517	85531	-16.0	ttcacctttcCTCCTGTTTATTCTTattaccccgt	nd	[COMP-HINF-SIMILAR-TO-CONSENSUS], [EXP-IDA-BINDING-OF-CELLULAR-EXTRACTS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS], [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [EXP-IMP-SITE-MUTATION], [COMP-HINF-SIMILAR-TO-CONSENSUS], [EXP-CHIP-SEQ], [EXP-IDA-BINDING-OF-CELLULAR-EXTRACTS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS], [EXP-IMP-SITE-MUTATION]	C	[4], [5], [6], [7], [8], [9], [10], [11], [12], [13]
proximal	Lrp	repressor	ilvIp2	85537	85551	5.0	ttcttattacCCCGTGTTTATGTCTctggctgcca	nd	[COMP-HINF-SIMILAR-TO-CONSENSUS], [EXP-IDA-BINDING-OF-CELLULAR-EXTRACTS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS], [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [EXP-IMP-SITE-MUTATION], [COMP-HINF-SIMILAR-TO-CONSENSUS], [EXP-CHIP-SEQ], [EXP-IDA-BINDING-OF-CELLULAR-EXTRACTS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS], [EXP-IMP-SITE-MUTATION]	C	[4], [5], [6], [7], [8], [9], [10], [11], [12], [13]

Transcription factor	Function	Promoter	Binding Sites	Evidence (Confirmed, Strong, Weak)	Reference(s)
Lrp	repressor	ilvIp2	85341	85355	-192.0	agacatgaatGTCTGGTTTATTCTGcattttttat	[COMP-HINF-SIMILAR-TO-CONSENSUS], [EXP-IDA-BINDING-OF-CELLULAR-EXTRACTS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS], [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [EXP-IMP-SITE-MUTATION]	[4], [5], [6], [7], [8], [9], [10], [11], [12], [13]
Lrp	repressor	ilvIp2	85372	85386	-161.0	ttattgaatgTAGAATTTTATTCTGaatgtgtggg	[COMP-HINF-SIMILAR-TO-CONSENSUS], [EXP-IDA-BINDING-OF-CELLULAR-EXTRACTS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS], [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [EXP-IEP-MICROARRAY], [EXP-IMP-SITE-MUTATION]	[4], [5], [6], [7], [8], [9], [10], [11], [12], [13], [14]
Lrp	repressor	ilvIp2	85453	85467	-80.0	ttgtgggattCAGCCGATTTATTATcaatttaatc	[COMP-HINF-SIMILAR-TO-CONSENSUS], [EXP-IDA-BINDING-OF-CELLULAR-EXTRACTS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS], [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [EXP-IMP-SITE-MUTATION]	[4], [5], [6], [7], [8], [9], [10], [11], [12], [13]
Lrp	repressor	ilvIp2	85488	85502	-45.0	ctctgtaatgGAGGATTTTATCGTTtcttttcacc	[COMP-HINF-SIMILAR-TO-CONSENSUS], [EXP-IDA-BINDING-OF-CELLULAR-EXTRACTS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS], [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [EXP-IMP-SITE-MUTATION]	[4], [5], [6], [7], [8], [9], [10], [11], [12], [13]
Lrp	repressor	ilvIp2	85517	85531	-16.0	ttcacctttcCTCCTGTTTATTCTTattaccccgt	[COMP-HINF-SIMILAR-TO-CONSENSUS], [EXP-IDA-BINDING-OF-CELLULAR-EXTRACTS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS], [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [EXP-IMP-SITE-MUTATION]	[4], [5], [6], [7], [8], [9], [10], [11], [12], [13]
Lrp	repressor	ilvIp2	85537	85551	5.0	ttcttattacCCCGTGTTTATGTCTctggctgcca	[COMP-HINF-SIMILAR-TO-CONSENSUS], [EXP-IDA-BINDING-OF-CELLULAR-EXTRACTS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS], [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [EXP-IMP-SITE-MUTATION]	[4], [5], [6], [7], [8], [9], [10], [11], [12], [13]

Allosteric regulation of RNA-polymerase

	Regulator	Function	Promoter target of RNApol	Growth Conditions	Note	Evidence	Reference
	ppGpp	inhibition	ilvIp2	nd		[EXP-IEP-GENE-EXPRESSION-ANALYSIS]	[15]

Evidence:	[EXP-IEP-GENE-EXPRESSION-ANALYSIS] Gene expression analysis
Reference(s):	[15] Baccigalupi L., et al., 1995

Transcription unit

Name:	ilvIH
Synonym(s):	OP00110
Gene(s):	ilvI, ilvH Genome Browser M3D Gene expression COLOMBOS
Note(s):	The ilvIH operon is activated by Lrp in a leucine-sensitive manner; activation is optimum in the absence of leucine, but it is reduced about 30-fold in its presence Wang Q,1993and by the effects of HNS Levinthal M,1994. Lrp binds to six sites, four proximal tandem sites and two distal sites, at the upstream region of the ilvIH operon Wang Q,1993 The binding of Lrp to the proximal sites is necessary for the activation of the operon, but it is not enough; Lrp must also bind to the distal sites in order to allow operon expression Jafri S,2002 The highest affinity of the interaction of Lrp upstream of ilvI has been observed at pH 8.0, although the cooperativity increases as the pH declines Ishii Y,2021.
Evidence:	[EXP-IDA-TRANSCRIPT-LEN-DETERMINATION] Length of transcript experimentally determined
Reference(s):	[1] Blattner FR., et al., 1997 [6] Jafri S., et al., 2002 [3] Squires CH., et al., 1983 [11] Wang Q., et al., 1993
Promoter
Name:	ilvIHp1
+1:	85600
Sigma Factor:	Sigma70 Sigmulon
Distance from start of the gene:	30
Sequence:	gtgtttatgtctctggctgccaattgcttaagcaagatcggacggttaatgtgttttacaCattttttccgtcaaacagtg -35 -10 +1
Evidence:	[COMP-AINF] [COMP-HINF-POSITIONAL-IDENTIFICATION] [EXP-IDA-TRANSCRIPTION-INIT-MAPPING] [EXP-IMP] [EXP-TAS]
Reference(s):	[4] Haughn GW., et al., 1985 [16] Huerta AM., et al., 2003 [7] Marasco R., et al., 1994 [11] Wang Q., et al., 1993 [5] Willins DA., et al., 1992

TF binding sites (TFBSs)

[H-NS,-] Phrase

Type	Transcription factor	Function	Promoter	Binding Sites				Growth Conditions	Evidence	Confidence level (C: Confirmed, S: Strong, W: Weak)	Reference(s)
Type	Transcription factor	Function	Promoter	LeftPos	RightPos	Central Rel-Pos	Sequence	Growth Conditions	Evidence		Reference(s)
nd	H-NS	repressor	ilvIHp1	nd	nd	nd	nd	nd	[EXP-IEP-GENE-EXPRESSION-ANALYSIS]	W	[18]

[Lrp,+] Phrase

Type	Transcription factor	Function	Promoter	Binding Sites				Growth Conditions	Evidence	Confidence level (C: Confirmed, S: Strong, W: Weak)	Reference(s)
Type	Transcription factor	Function	Promoter	LeftPos	RightPos	Central Rel-Pos	Sequence	Growth Conditions	Evidence		Reference(s)
remote	Lrp	activator	ilvIHp1	85341	85355	-252.0	agacatgaatGTCTGGTTTATTCTGcattttttat	nd	[EXP-IEP-GENE-EXPRESSION-ANALYSIS], [EXP-IEP-RNA-SEQ], [COMP-HINF-SIMILAR-TO-CONSENSUS], [EXP-CHIP-SEQ], [EXP-IDA-BINDING-OF-CELLULAR-EXTRACTS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS], [EXP-IMP-SITE-MUTATION]	C	[4], [5], [6], [7], [8], [9], [10], [11], [12], [13], [17]
remote	Lrp	activator	ilvIHp1	85372	85386	-221.0	ttattgaatgTAGAATTTTATTCTGaatgtgtggg	nd	[EXP-IEP-GENE-EXPRESSION-ANALYSIS], [EXP-IEP-MICROARRAY], [EXP-IEP-RNA-SEQ], [COMP-AINF-PATTERN-DISCOVERY], [COMP-HINF-SIMILAR-TO-CONSENSUS], [EXP-CHIP-CHIP], [EXP-CHIP-SEQ], [EXP-IDA-BINDING-OF-CELLULAR-EXTRACTS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS], [EXP-IMP-SITE-MUTATION]	C	[4], [5], [6], [7], [8], [9], [10], [11], [12], [13], [14], [17]
remote	Lrp	activator	ilvIHp1	85453	85467	-140.0	ttgtgggattCAGCCGATTTATTATcaatttaatc	nd	[EXP-IEP-GENE-EXPRESSION-ANALYSIS], [EXP-IEP-RNA-SEQ], [COMP-HINF-SIMILAR-TO-CONSENSUS], [EXP-CHIP-SEQ], [EXP-IDA-BINDING-OF-CELLULAR-EXTRACTS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS], [EXP-IMP-SITE-MUTATION]	C	[4], [5], [6], [7], [8], [9], [10], [11], [12], [13], [17]
remote	Lrp	activator	ilvIHp1	85488	85502	-105.0	ctctgtaatgGAGGATTTTATCGTTtcttttcacc	nd	[EXP-IEP-GENE-EXPRESSION-ANALYSIS], [EXP-IEP-RNA-SEQ], [COMP-HINF-SIMILAR-TO-CONSENSUS], [EXP-CHIP-SEQ], [EXP-IDA-BINDING-OF-CELLULAR-EXTRACTS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS], [EXP-IMP-SITE-MUTATION]	C	[4], [5], [6], [7], [8], [9], [10], [11], [12], [13], [17]
proximal	Lrp	activator	ilvIHp1	85517	85531	-76.0	ttcacctttcCTCCTGTTTATTCTTattaccccgt	nd	[EXP-IEP-GENE-EXPRESSION-ANALYSIS], [EXP-IEP-RNA-SEQ], [COMP-HINF-SIMILAR-TO-CONSENSUS], [EXP-CHIP-SEQ], [EXP-IDA-BINDING-OF-CELLULAR-EXTRACTS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS], [EXP-IMP-SITE-MUTATION]	C	[4], [5], [6], [7], [8], [9], [10], [11], [12], [13], [17]
proximal	Lrp	activator	ilvIHp1	85537	85551	-56.0	ttcttattacCCCGTGTTTATGTCTctggctgcca	nd	[EXP-IEP-GENE-EXPRESSION-ANALYSIS], [EXP-IEP-RNA-SEQ], [COMP-HINF-SIMILAR-TO-CONSENSUS], [EXP-CHIP-SEQ], [EXP-IDA-BINDING-OF-CELLULAR-EXTRACTS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS], [EXP-IMP-SITE-MUTATION]	C	[4], [5], [6], [7], [8], [9], [10], [11], [12], [13], [17]

Transcription factor	Function	Promoter	Binding Sites	Evidence (Confirmed, Strong, Weak)	Reference(s)
Lrp	activator	ilvIHp1	85341	85355	-252.0	agacatgaatGTCTGGTTTATTCTGcattttttat	[EXP-IEP-GENE-EXPRESSION-ANALYSIS], [EXP-IEP-RNA-SEQ]	[4], [5], [6], [7], [8], [9], [10], [11], [12], [13], [17]
Lrp	activator	ilvIHp1	85372	85386	-221.0	ttattgaatgTAGAATTTTATTCTGaatgtgtggg	[EXP-IEP-GENE-EXPRESSION-ANALYSIS], [EXP-IEP-MICROARRAY], [EXP-IEP-RNA-SEQ]	[4], [5], [6], [7], [8], [9], [10], [11], [12], [13], [14], [17]
Lrp	activator	ilvIHp1	85453	85467	-140.0	ttgtgggattCAGCCGATTTATTATcaatttaatc	[EXP-IEP-GENE-EXPRESSION-ANALYSIS], [EXP-IEP-RNA-SEQ]	[4], [5], [6], [7], [8], [9], [10], [11], [12], [13], [17]
Lrp	activator	ilvIHp1	85488	85502	-105.0	ctctgtaatgGAGGATTTTATCGTTtcttttcacc	[EXP-IEP-GENE-EXPRESSION-ANALYSIS], [EXP-IEP-RNA-SEQ]	[4], [5], [6], [7], [8], [9], [10], [11], [12], [13], [17]
Lrp	activator	ilvIHp1	85517	85531	-76.0	ttcacctttcCTCCTGTTTATTCTTattaccccgt	[EXP-IEP-GENE-EXPRESSION-ANALYSIS], [EXP-IEP-RNA-SEQ]	[4], [5], [6], [7], [8], [9], [10], [11], [12], [13], [17]
Lrp	activator	ilvIHp1	85537	85551	-56.0	ttcttattacCCCGTGTTTATGTCTctggctgcca	[EXP-IEP-GENE-EXPRESSION-ANALYSIS], [EXP-IEP-RNA-SEQ]	[4], [5], [6], [7], [8], [9], [10], [11], [12], [13], [17]
H-NS	repressor	ilvIHp1	nd	nd	nd	nd	[EXP-IEP-GENE-EXPRESSION-ANALYSIS]	[18]

RNA cis-regulatory element

Regulation, transcriptional elongation
Attenuator type:	Transcriptional
Strand:	forward

Structure type	Energy	LeftPos	RightPos	Sequence (RNA-strand)
terminator	-6.9	85476	85498	tcaatttaatCCTCTGTAATGGAGGATTTTATcgtttctttt
anti-terminator	-4.8	85447	85479	gtaagttgtgGGATTCAGCCGATTTATTATCAATTTAATCCTctgtaatgga
anti-anti-terminator	-3.6	85430	85462	aaaatagagaAATTGCTGTAAGTTGTGGGATTCAGCCGATTTattatcaatt

Notes: "The provided "Sequence" is that of the RNA strand, i.e. U's are shown instead of T's and regulators on the reverse strand will appear as the reverse complement of the sequence delimited by LeftPos-RigtPos"

Evidence
[COMP-HINF-POSITIONAL-IDENTIFICATION] Human inference of promoter position
[EXP-IDA-TRANSCRIPTION-INIT-MAPPING] Transcription initiation mapping
[EXP-IMP] Inferred from mutant phenotype
[COMP-HINF-SIMILAR-TO-CONSENSUS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.
[EXP-IDA-BINDING-OF-CELLULAR-EXTRACTS] Binding of cellular extracts
[EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] Binding of purified proteins
[EXP-IEP-GENE-EXPRESSION-ANALYSIS] Gene expression analysis
[EXP-IMP-SITE-MUTATION] Site mutation
[EXP-CHIP-SEQ] ChIP-seq evidence
[EXP-IEP-MICROARRAY] Expression microarray evidence
[COMP-AINF-PATTERN-DISCOVERY] Automated inference based on sequence pattern discovery
[EXP-CHIP-CHIP] ChIP-chip evidence
[COMP-AINF] Inferred computationally without human oversight
[EXP-TAS] Traceable author statement to experimental support
[EXP-IEP-RNA-SEQ] RNA-seq evidence

Reference(s)
[1] Blattner FR., Plunkett G., Bloch CA., Perna NT., Burland V., Riley M., Collado-Vides J., Glasner JD., Rode CK., Mayhew GF., Gregor J., Davis NW., Kirkpatrick HA., Goeden MA., Rose DJ., Mau B., Shao Y., 1997, The complete genome sequence of Escherichia coli K-12., Science 277(5331):1453-74
[2] Lago CT., Sannia G., Marino G., Squires CH., Calvo JM., De Felice M., 1985, The ilvIH operon of Escherichia coli K-12. Identification of the gene products and recognition of the translational start by polypeptide microsequencing., Biochim Biophys Acta 824(1):74-9
[3] Squires CH., De Felice M., Devereux J., Calvo JM., 1983, Molecular structure of ilvIH and its evolutionary relationship to ilvG in Escherichia coli K12., Nucleic Acids Res 11(15):5299-313
[4] Haughn GW., Squires CH., De Felice M., Largo CT., Calvo JM., 1985, Unusual organization of the ilvIH promoter of Escherichia coli., J Bacteriol 163(1):186-98
[5] Willins DA., Calvo JM., 1992, In vitro transcription from the Escherichia coli ilvIH promoter., J Bacteriol 174(23):7648-55
[6] Jafri S., Chen S., Calvo JM., 2002, ilvIH operon expression in Escherichia coli requires Lrp binding to two distinct regions of DNA., J Bacteriol 184(19):5293-300
[7] Marasco R., Varcamonti M., La Cara F., Ricca E., De Felice M., Sacco M., 1994, In vivo footprinting analysis of Lrp binding to the ilvIH promoter region of Escherichia coli., J Bacteriol 176(17):5197-201
[8] Platko JV., Calvo JM., 1993, Mutations affecting the ability of Escherichia coli Lrp to bind DNA, activate transcription, or respond to leucine., J Bacteriol 175(4):1110-7
[9] Platko JV., Willins DA., Calvo JM., 1990, The ilvIH operon of Escherichia coli is positively regulated., J Bacteriol 172(8):4563-70
[10] Ricca E., Aker DA., Calvo JM., 1989, A protein that binds to the regulatory region of the Escherichia coli ilvIH operon., J Bacteriol 171(3):1658-64
[11] Wang Q., Calvo JM., 1993, Lrp, a global regulatory protein of Escherichia coli, binds co-operatively to multiple sites and activates transcription of ilvIH., J Mol Biol 229(2):306-18
[12] Wang Q., Calvo JM., 1993, Lrp, a major regulatory protein in Escherichia coli, bends DNA and can organize the assembly of a higher-order nucleoprotein structure., EMBO J 12(6):2495-501
[13] Wang Q., Sacco M., Ricca E., Lago CT., De Felice M., Calvo JM., 1993, Organization of Lrp-binding sites upstream of ilvIH in Salmonella typhimurium., Mol Microbiol 7(6):883-91
[14] Cho BK., Barrett CL., Knight EM., Park YS., Palsson BO., 2008, Genome-scale reconstruction of the Lrp regulatory network in Escherichia coli., Proc Natl Acad Sci U S A 105(49):19462-7
[15] Baccigalupi L., Marasco R., Ricca E., De Felice M., Sacco M., 1995, Control of ilvIH transcription during amino acid downshift in stringent and relaxed strains of Escherichia coli., FEMS Microbiol Lett 131(1):95-8
[16] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78
[17] Kroner GM., Wolfe MB., Freddolino PL., 2019, Escherichia coli Lrp Regulates One-Third of the Genome via Direct, Cooperative, and Indirect Routes., J Bacteriol 201(3)
[18] Levinthal M., Lejeune P., Danchin A., 1994, The H-NS protein modulates the activation of the ilvIH operon of Escherichia coli K12 by Lrp, the leucine regulatory protein., Mol Gen Genet 242(6):736-43

RegulonDB