RegulonDB RegulonDB 10.8: Operon Form

purEK operon and associated TUs in Escherichia coli K-12 genome

Name: purEK
This page displays every known transcription unit of this operon and their known regulation.

Transcription unit          
Name: purEK
Synonym(s): OP00112, purE
Gene(s): purK, purE   Genome Browser M3D Gene expression COLOMBOS
Reference(s): [1] Koduri RK., et al., 1980
[2] Meng LM., et al., 1990
[3] Watanabe W., et al., 1989
Name: purEp
+1: 553142
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 42
Sequence: gctgatttcacagccacgcaaccgttttccttgctctctttccgtgctattctctgtgccCtctaaagccgagagttgtgc
                          -35                   -10         +1                   
Note(s): 554 promoter regions from Escherichia coli were aligned in order to analyze their sequence similarities. Mitchell et al. (2003) Mitchell JE,2003 focused on conservation, patterns, similarities, and differences between promoters with or without an extended -10 5'-TG-3' element and other conserved elements. They experimentally showed that, for several naturally occurring extended -10 promoters, the 5'-TRTG-3' motif is an important determinant for promoter activity. Eleven promoters were selected for further experimental study. Of those, the seven most active (aroF > ompF > envA > purFp1 > purEF > purMN > gyrA) were investigated for the contribution of the extended -10 motif by changing the 5'-TG-3'. Promoters with poor matches to the -10 and -35 consensus hexamers are more dependent on the 5'-TG-3' motif and this plays a similar role on different promoters. The dinucleotide at -17 and -16 in aroFp results in similar patterns of activity, suggesting that these bases play a similar role on different promoters Mitchell JE,2003.
Evidence: [IMP]
Reference(s): [4] He B., et al., 1990
[5] Mitchell JE., et al., 2003
[6] Tiedeman AA., et al., 1989
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal PurR-hypoxanthine repressor purEp 553172 553187 -37.5 tttcacagccACGCAACCGTTTTCCTtgctctcttt nd [AIBSCS], [APIORCISFBSCS], [BCE], [CV(GEA/ROMA)], [GEA] [2], [3], [4], [7]