RegulonDB RegulonDB 11.0: Operon Form
   

tyrB operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: tyrB
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: tyrB
Synonym(s): OP00123
Gene(s): tyrB   Genome Browser M3D Gene expression COLOMBOS
Note(s): When phenylalanine or tyrosine along with ATP interact with the TyrR protein, the transcription of the tyrB gene is repressed through the binding of aTyrR hexamer to a weak and strong TyrR box located 19.5 and 42.5 bp downstream of the tyrBp promoter, respectively Yang J,2002
The two aromatic amino acids affect the transcription in different ways. While tyrosine prevents the open complex formation, phenylalanine affects one or more steps after the open complex formationYang J,2002
The expression of the gene tyrB is enhanced under oxygen limitation Kawaguchi H, Miyagawa H, Nakamura-Tsuruta S, Takaya N, Ogino C, Kondo A,2019.
Evidence: [BTEI] Boundaries of transcription experimentally identified
Reference(s): [1] Fotheringham IG., et al., 1986
[2] Yang J., et al., 1987
Promoter
Name: tyrBp
+1: 4267082
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 32
Sequence: ttgccgttcgctcctgaacatccactcgatcttcgccttcttccggtttattgtgttttaAccacctgcccgtaaacctgg
                         -35                     -10        +1                   
Evidence: [CV(RPF/RS-EPT-CBR)]
[CV(RPF/TIM)]
[HIPP]
[ICWHO]
[RPF]
[RS-EPT-CBR]
[TIM]
Reference(s): [3] Huerta AM., et al., 2003
[4] Salgado H, et al., 2012
[5] Yang J., et al., 2002
[2] Yang J., et al., 1987
Terminator(s)
Type: rho-independent
Sequence: atgcaggaaaGCAGGCTGGAGCTACCCAGCCTGCagtgaaatta
Reference(s): [1] Fotheringham IG., et al., 1986
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal TyrR-L-tyrosine repressor tyrBp 4267092 4267109 19.5 accacctgccCGTAAACCTGGAGAACCAtcgcgtgttt nd [GEA], [APIORCISFBSCS], [BPP], [SM] [2], [5]
remote TyrR-L-tyrosine repressor tyrBp 4267115 4267132 42.5 aaccatcgcgTGTTTCAAAAAGTTGACGcctacgctgg nd [GEA], [APIORCISFBSCS], [BPP], [SM] [2], [5]




Reference(s)    

 [1] Fotheringham IG., Dacey SA., Taylor PP., Smith TJ., Hunter MG., Finlay ME., Primrose SB., Parker DM., Edwards RM., 1986, The cloning and sequence analysis of the aspC and tyrB genes from Escherichia coli K12. Comparison of the primary structures of the aspartate aminotransferase and aromatic aminotransferase of E. coli with those of the pig aspartate aminotransferase isoenzymes., Biochem J 234(3):593-604

 [2] Yang J., Pittard J., 1987, Molecular analysis of the regulatory region of the Escherichia coli K-12 tyrB gene., J Bacteriol 169(10):4710-5

 [3] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [4] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [5] Yang J., Camakaris H., Pittard J., 2002, Molecular analysis of tyrosine-and phenylalanine-mediated repression of the tyrB promoter by the TyrR protein of Escherichia coli., Mol Microbiol 45(5):1407-19


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