RegulonDB RegulonDB 11.1: Operon Form
   

malT operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: malT
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: malT
Synonym(s): OP00128
Gene(s): malT   Genome Browser M3D Gene expression COLOMBOS
Note(s):
The translation of the malT mRNA is positively controlled by H-NS and StpA regulators Johansson J, Dagberg B, Richet E, Uhlin BE,1998. Park HS, Ostberg Y, Johansson J, Wagner EG, Uhlin BE,2010
Evidence: [EXP-IDA-BOUNDARIES-DEFINED] Boundaries of transcription experimentally identified
Reference(s): [1] Cole ST., et al., 1986
[2] Kornacker MG., et al., 1989
Promoter
Name: malTp
+1: 3553024
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 61
Sequence: cagacacataaaaaaacgtcatcgcttgcattagaaaggtttctggccgaccttataaccAttaattacgaagcgcaaaaa
                          -35                    -10        +1                   
Evidence: [COMP-AINF]
[EXP-IDA-TRANSCRIPTION-INIT-MAPPING]
[RS-EPT-CBR]
Reference(s): [3] Chapon C. 1982
[4] Chapon C., et al., 1983
[5] Dethiollaz S., et al., 1996
[6] Huerta AM., et al., 2003
[7] Salgado H, et al., 2012
Terminator(s)
Type: rho-independent
Sequence: gtgtaagtttAGCCGGATAACGCGCCAGATCCGGCTtacatctctg
Reference(s): [1] Cole ST., et al., 1986
TF binding sites (TFBSs)
[CRP,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence Confidence level (C: Confirmed, S: Strong, W: Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal CRP-cyclic-AMP activator malTp 3552943 3552964 -70.5 aaagatttggAATTGTGACACAGTGCAAATTCagacacataa nd [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [COMP-HINF-SIMILAR-TO-CONSENSUS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] W [4], [8], [9], [10], [11]
[Lrp,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence Confidence level (C: Confirmed, S: Strong, W: Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd Lrp activator malTp nd nd nd nd nd [EXP-IEP-GENE-EXPRESSION-ANALYSIS] W [12]
[Mlc,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence Confidence level (C: Confirmed, S: Strong, W: Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal Mlc repressor malTp 3553024 3553046 12.0 ccttataaccATTAATTACGAAGCGCAAAAAAAataatatttc nd [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [COMP-HINF-SIMILAR-TO-CONSENSUS], [EXP-IDA-BINDING-OF-CELLULAR-EXTRACTS] S [8]


Evidence    

 [COMP-AINF] Inferred computationally without human oversight

 [EXP-IDA-TRANSCRIPTION-INIT-MAPPING] Transcription initiation mapping

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [EXP-IEP-GENE-EXPRESSION-ANALYSIS] Gene expression analysis

 [COMP-HINF-SIMILAR-TO-CONSENSUS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.

 [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] Binding of purified proteins

 [EXP-IDA-BINDING-OF-CELLULAR-EXTRACTS] Binding of cellular extracts


Reference(s)    

 [1] Cole ST., Raibaud O., 1986, The nucleotide sequence of the malT gene encoding the positive regulator of the Escherichia coli maltose regulon., Gene 42(2):201-8

 [2] Kornacker MG., Boyd A., Pugsley AP., Plastow GS., 1989, A new regulatory locus of the maltose regulon in Klebsiella pneumoniae strain K21 identified by the study of pullulanase secretion mutants., J Gen Microbiol 135(Pt 2):397-408

 [3] Chapon C., 1982, Expression of malT, the regulator gene of the maltose region in Escherichia coli, is limited both at transcription and translation., EMBO J 1(3):369-74

 [4] Chapon C., Kolb A., 1983, Action of CAP on the malT promoter in vitro., J Bacteriol 156(3):1135-43

 [5] Dethiollaz S., Eichenberger P., Geiselmann J., 1996, Influence of DNA geometry on transcriptional activation in Escherichia coli., EMBO J 15(19):5449-58

 [6] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [7] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [8] Decker K., Plumbridge J., Boos W., 1998, Negative transcriptional regulation of a positive regulator: the expression of malT, encoding the transcriptional activator of the maltose regulon of Escherichia coli, is negatively controlled by Mlc., Mol Microbiol 27(2):381-90

 [9] Eichenberger P., Dethiollaz S., Buc H., Geiselmann J., 1997, Structural kinetics of transcription activation at the malT promoter of Escherichia coli by UV laser footprinting., Proc Natl Acad Sci U S A 94(17):9022-7

 [10] Eichenberger P., Dethiollaz S., Fujita N., Ishihama A., Geiselmann J., 1996, Influence of the location of the cAMP receptor protein binding site on the geometry of a transcriptional activation complex in Escherichia coli., Biochemistry 35(48):15302-12

 [11] Menendez M., Kolb A., Buc H., 1987, A new target for CRP action at the malT promoter., EMBO J 6(13):4227-34

 [12] Tchetina E., Newman EB., 1995, Identification of Lrp-regulated genes by inverse PCR and sequencing: regulation of two mal operons of Escherichia coli by leucine-responsive regulatory protein., J Bacteriol 177(10):2679-83

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