RegulonDB RegulonDB 11.1: Operon Form
   

speC operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: speC
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: speC
Synonym(s): OP00166
Gene(s): speC   Genome Browser M3D Gene expression COLOMBOS
Reference(s): [1] Wright JM., et al., 1986
Promoter
Name: speCp
+1: 3109175
Sigma Factor: Sigma38 Sigmulon
Distance from start of the gene: 20
Sequence: accagtttgacccatatctcatggggcagggttttccaccttgtccggtattcttacttcCccgaaacgggtttgcgctta
                                -35                   -10   +1                   
Evidence: [COMP-AINF]
[COMP-AINF-POSITIONAL-IDENTIFICATION]
[COMP-HINF-POSITIONAL-IDENTIFICATION]
Reference(s): [2] Boyle SM., et al., 1994
[3] Huerta AM., et al., 2003
TF binding sites (TFBSs)
[CRP,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence Confidence level (C: Confirmed, S: Strong, W: Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd CRP-cyclic-AMP repressor speCp nd nd nd nd nd [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [EXP-IMP] W [1], [4]
Allosteric regulation of RNA-polymerase
  Regulator Function Promoter target of RNApol Growth Conditions Note Evidence Reference
  ppGpp inhibition speCp nd   [EXP-IEP-GENE-EXPRESSION-ANALYSIS]
[EXP-IMP] 		[5]
Evidence: [EXP-IEP-GENE-EXPRESSION-ANALYSIS] Gene expression analysis
[EXP-IMP] Inferred from mutant phenotype
Reference(s): [5] Holtta E., et al., 1974


Evidence    

 [COMP-AINF] Inferred computationally without human oversight

 [COMP-AINF-POSITIONAL-IDENTIFICATION] Automated inference of promoter position

 [COMP-HINF-POSITIONAL-IDENTIFICATION] Human inference of promoter position

 [EXP-IEP-GENE-EXPRESSION-ANALYSIS] Gene expression analysis

 [EXP-IMP] Inferred from mutant phenotype


Reference(s)    

 [1] Wright JM., Satishchandran C., Boyle SM., 1986, Transcription of the speC (ornithine decarboxylase) gene of Escherichia coli is repressed by cyclic AMP and its receptor protein., Gene 44(1):37-45

 [2] Boyle SM., Barroso L., Moore RC., Wright JM., Patel T., 1994, Primary structure of the speC gene encoding biosynthetic ornithine decarboxylase in Escherichia coli., Gene 151(1-2):157-60

 [3] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [4] Wright JM., Boyle SM., 1982, Negative control of ornithine decarboxylase and arginine decarboxylase by adenosine-3':5'-cyclic monophosphate in Escherichia coli., Mol Gen Genet 186(4):482-7

 [5] Holtta E., Janne J., Pispa J., 1974, The regulation of polyamine synthesis during the stringent control in Escherichia coli., Biochem Biophys Res Commun 59(3):1104-11

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