RegulonDB RegulonDB 10.9: Operon Form
   

uhpT operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: uhpT
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: uhpT
Synonym(s): OP00173
Gene(s): uhpT   Genome Browser M3D Gene expression COLOMBOS
Note(s): uhpT gene expression is induced by glucose-6P or pyruvate and is inhibited by high phosphate concentrations Verhamme DT,2002
Reference(s): [1] Merkel TJ., et al., 1992
Promoter
Name: uhpTp
+1: 3847198
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 31
Sequence: ccaggttttgcctggacgctatctcaggcctgatttgctgctgatttttacaatgcatgcCtcacgcaggtattcatttca
                       -35                       -10        +1                   
Evidence: [HIPP]
[ICWHO]
[TIM]
Reference(s): [2] Huerta AM., et al., 2003
[1] Merkel TJ., et al., 1992
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote CRP-cAMP activator uhpTp 3847291 3847312 -103.5 gacaccgcgcGAAGCGTGATGCATCTCACCTTTtcacttcata nd [APIORCISFBSCS], [BCE], [BPP], [GEA], [SM] [3]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal UhpA-Pasp1 activator uhpTp 3847230 3847249 -41.5 ttccaggtttTGCCTGGACGCTATCTCAGGCctgatttgct nd [BCE], [BPP], [GEA], [SM] [3], [5], [7], [8]
proximal UhpA-Pasp2 activator uhpTp 3847253 3847272 -64.5 atatccggcaAAACTAAGAAATTTTCCAGGTtttgcctgga nd [BPP], [GEA], [SM] [4], [5], [6]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd UvrY-Phosphorylated activator uhpTp nd nd nd nd nd [BPP], [GEA], [IGI] [9]
Note(s): 1Previous studies have supported a model in which membrane-localized UhpC binds external Glu6P and transmits a signal to UhpB, which subsequently converts UhpA to a form capable of activating transcription at the uhpT promoter. Each dimer binds to an inverted repeat of 24 bp Olekhnovich IN,2002 UhpB inhibits UhpA action in the uhpT promoter Wright JS,2000
2Previous studies have supported a model in which membrane-localized UhpC binds external Glu6P and transmits a signal to UhpB, which subsequently converts UhpA to a form capable of activating transcription at the uhpT promoter. Each dimer binds to an inverted repeat of 24 bp Olekhnovich IN,2002 UhpB inhibits UhpA action in the uhpT promoter Wright JS,20002Previous studies have supported a model in which membrane-localized UhpC binds external Glu6P and transmits a signal to UhpB, which subsequently converts UhpA to a form capable of activating transcription at the uhpT promoter. Each dimer binds to an inverted repeat of 24 bp Olekhnovich IN,2002 UhpB inhibits UhpA action in the uhpT promoter Wright JS,2000
3Previous studies have supported a model in which membrane-localized UhpC binds external Glu6P and transmits a signal to UhpB, which subsequently converts UhpA to a form capable of activating transcription at the uhpT promoter. Each dimer binds to an inverted repeat of 24 bp Olekhnovich IN,2002 UhpB inhibits UhpA action in the uhpT promoter Wright JS,2000




Reference(s)    

 [1] Merkel TJ., Nelson DM., Brauer CL., Kadner RJ., 1992, Promoter elements required for positive control of transcription of the Escherichia coli uhpT gene., J Bacteriol 174(9):2763-70

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [3] Olekhnovich IN., Kadner RJ., 2002, Mutational scanning and affinity cleavage analysis of UhpA-binding sites in the Escherichia coli uhpT promoter., J Bacteriol 184(10):2682-91

 [4] Chen Q., Kadner RJ., 2000, Effect of altered spacing between uhpT promoter elements on transcription activation., J Bacteriol 182(16):4430-6

 [5] Dahl JL., Wei BY., Kadner RJ., 1997, Protein phosphorylation affects binding of the Escherichia coli transcription activator UhpA to the uhpT promoter., J Biol Chem 272(3):1910-9

 [6] Wright JS., Olekhnovich IN., Touchie G., Kadner RJ., 2000, The histidine kinase domain of UhpB inhibits UhpA action at the Escherichia coli uhpT promoter., J Bacteriol 182(22):6279-86

 [7] Olekhnovich IN., Dahl JL., Kadner RJ., 1999, Separate contributions of UhpA and CAP to activation of transcription of the uhpT promoter of Escherichia coli., J Mol Biol 292(5):973-86

 [8] Verhamme DT., Postma PW., Crielaard W., Hellingwerf KJ., 2002, Cooperativity in signal transfer through the Uhp system of Escherichia coli., J Bacteriol 184(15):4205-10

 [9] Yoshida M., Ishihama A., Yamamoto K., 2015, Cross talk in promoter recognition between six NarL-family response regulators of Escherichia coli two-component system., Genes Cells 20(7):601-12


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