|
|
| Operon |  |
|---|---|
| Name: | malS |
| This page displays every known transcription unit of this operon and their known regulation. | |
| Name: | malS | |||||||||
| Synonym(s): | OP00184 | |||||||||
| Gene(s): | malS Genome Browser M3D Gene expression COLOMBOS | |||||||||
| Note(s): | The malS gene, which codes for a periplasmic α-amylase, is an isolated gene Freundlieb S,1986, and it is regulated by the maltose regulator, MalT Freundlieb S,1986, the catabolite repressor, Crp Bukau B,1986, and the Mg deprivation response cognate, PhoP Monsieurs P,2005. Genes flanking malS are encoded in opposite directions: the downstream gene,avtA, which codes for an alanine-valine transaminase Wang MD,1987, and the bax gene. | |||||||||
| Evidence: | [AISGDTU] Automated inference that a single-gene directon is a transcription unit | |||||||||
| Reference(s): |
[1] Bukau B., et al., 1986 [2] Freundlieb S., et al., 1986 [3] Monsieurs P., et al., 2005 [4] Otsuka J., et al., 1996 [5] Schneider E., et al., 1992 [6] Wang MD., et al., 1987 |
|||||||||
| Promoter | ||||||||||
| Name: | malSp | |||||||||
| +1: | 3737470 | |||||||||
| Sigma Factor: | Sigma70 Sigmulon | |||||||||
| Distance from start of the gene: | 27 | |||||||||
| Sequence: |
cctgtggacttaccgctcagggatgagttttgtttggcttatcgctggcaaactgtctgaAatcgcagcaataaggactca -35 -10 +1 |
|||||||||
| Evidence: |
[ICWHO] [TIM] |
|||||||||
| Reference(s): |
[7] Huerta AM., et al., 2003 [5] Schneider E., et al., 1992 |
|||||||||
| Terminator(s) | ||||||||||
| Type: | rho-independent | |||||||||
| Sequence: | aacagtaactTTTCCGGCTTCCCGTTCGTCAGTACCTCGGGAAGCCGCCAAccaggataaa | |||||||||
| Reference(s): | [5] Schneider E., et al., 1992 | |||||||||
| Type: | rho-independent | |||||||||
| Sequence: | cagccctaatCAGCGTTGCAGGATAAAGCACCGCTCactcttcaac | |||||||||
| Reference(s): | [5] Schneider E., et al., 1992 | |||||||||
| Type | Transcription factor | Function | Promoter | Binding Sites | Growth Conditions | Evidence (Confirmed, Strong, Weak) | Reference(s) | |||
|---|---|---|---|---|---|---|---|---|---|---|
| LeftPos | RightPos | Central Rel-Pos | Sequence | |||||||
| proximal | MalT-maltotriose-ATP | activator | malSp | 3737391 | 3737400 | -74.5 | gcgcgagatcGCTCACCCTTGcttctcatcc | nd | [AIBSCS] | nd |
| proximal | MalT-maltotriose-ATP | activator | malSp | 3737404 | 3737413 | -61.5 | cacccttgctTCTCATCCTGTggacttaccg | nd | [AIBSCS] | nd |
| proximal | MalT-maltotriose-ATP3 | activator | malSp | 3737428 | 3737437 | -37.5 | cttaccgctcAGGGATGAGTTttgtttggct | nd | [AIBSCS], [BCE] | nd |
| Type | Transcription factor | Function | Promoter | Binding Sites | Growth Conditions | Evidence (Confirmed, Strong, Weak) | Reference(s) | |||
|---|---|---|---|---|---|---|---|---|---|---|
| LeftPos | RightPos | Central Rel-Pos | Sequence | |||||||
| remote | PhoP-Phosphorylated1 | activator | malSp | 3737301 | 3737317 | -161.0 | caaatctgaaACTATGTCACGTGTTAACgattcagatt | nd | [AIBSCS], [GEA] | [3] |
| Note(s): |
1This region contains a possible cAMP/CAP-binding site. Because the site is far upstream of the promoter and occurs only as a single copy, in contrast to the three copies in a typical cAMP/CAP-controlled mal promoter, it is unlikely that it is functional in the expression of malS.3This site has 80% homology to the MalT consensus.1This regulatory interaction was identified by means of PhoPQ-related microarray experiments, which checked for the presence of the regulatory motif in the promoter region Monsieurs P,2005.1This region contains a possible cAMP/CAP-binding site. Because the site is far upstream of the promoter and occurs only as a single copy, in contrast to the three copies in a typical cAMP/CAP-controlled mal promoter, it is unlikely that it is functional in the expression of malS. 2This regulatory interaction was identified by means of PhoPQ-related microarray experiments, which checked for the presence of the regulatory motif in the promoter region Monsieurs P,2005. 5This site has 80% homology to the MalT consensus. |
| RNA cis-regulatory element | |
|---|
| Regulation, transcriptional elongation | |
| Attenuator type: | Transcriptional |
| Strand: | forward |
| Structure type | Energy | LeftPos | RightPos | Sequence (RNA-strand) | |
|---|---|---|---|---|---|
| terminator | -14.5 | 3737405 | 3737444 | cccttgcttcTCATCCTGTGGACTTACCGCTCAGGGATGAGTTTTGTTTggcttatcgc | |
| anti-terminator | -6.3 | 3737378 | 3737417 | cagccgttttTTGCGCGAGATCGCTCACCCTTGCTTCTCATCCTGTGGActtaccgctc | |
| anti-anti-terminator | -13.8 | 3737349 | 3737396 | gcagaaaatgTGGGGGTTATCGCAAAATTCAGCCGTTTTTTGCGCGAGATCGCTCACccttgcttct |
| Notes: "The provided "Sequence" is that of the RNA strand, i.e. U's are shown instead of T's and regulators on the reverse strand will appear as the reverse complement of the sequence delimited by LeftPos-RigtPos" |
| Reference(s) |
|
|---|---|
[ICWHO] Inferred computationally without human oversight