RegulonDB RegulonDB 10.9: Operon Form
   

cysPUWAM operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: cysPUWAM
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: cysPUWAM
Synonym(s): OP00195, cysPTWAM
Gene(s): cysM, cysA, cysW, cysU, cysP   Genome Browser M3D Gene expression COLOMBOS
Note(s): cysPUWAM expression is inhibited by oleanolic and ursolic acid, which in turn affect the expression of the cysteine regulon and the stress response Grudniak AM,2011 cysPUW as well as other genes involved in sulfur metabolism were significantly upregulated in a MG1655 lysogen carrying the Stx2a phage PA8 1208335|.
The cysP gene is upregulated at 1.25 M MgSO4 (Magnesium Sulfate), which suggests that cells control the uptake of sulfate by upregulating the expression of cysP 32349403
Reference(s): [1] Hryniewicz M., et al., 1990
[2] Sirko A., et al., 1990
Promoter
Name: cysPp
+1: 2543557
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 29
Sequence: accaaacggtatataaaaccgttactcctttcacgtccgttataaatatgatggctattaGaaagtcattaaatttataag
                      -35                      -10          +1                   
Evidence: [ICWHO]
[TIM]
Reference(s): [1] Hryniewicz M., et al., 1990
[3] Huerta AM., et al., 2003
TF binding sites (TFBSs)
[CysB,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal CysB-O-acetyl-L-serine activator cysPp 2543622 2543621 -65.0 ttcaacttccAaatcaccaaa nd [BPP], [GEA] [4], [5], [6]
[H-NS,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd H-NS repressor cysPp nd nd nd nd nd [BPP], [GEA], [IGI] [7]


RNA cis-regulatory element    
Regulation, transcriptional elongation  
Attenuator type: Transcriptional
Strand: reverse
  Structure type Energy LeftPos RightPos Sequence (RNA-strand)
  terminator -18.5 2539662 2539695 aatgttaaacGCCCGGAGGCGCTTCCCGCGATCCGGGCTTTTTaatggcaagg
  anti-terminator -13.3 2539682 2539726 ctctcgcacaAAGCGCCTGATAGGTTGAGTGAATGTTAAACGCCCGGAGGCGCTtcccgcgatc
Notes: "The provided "Sequence" is that of the RNA strand, i.e. U's are shown instead of T's and regulators on the reverse strand will appear as the reverse complement of the sequence delimited by LeftPos-RigtPos"


Evidence    

 [ICWHO] Inferred computationally without human oversight

 [TIM] Transcription initiation mapping

 [BPP] Binding of purified proteins

 [GEA] Gene expression analysis

 [IGI] Inferred from genetic interaction


Reference(s)    

 [1] Hryniewicz M., Sirko A., Palucha A., Bock A., Hulanicka D., 1990, Sulfate and thiosulfate transport in Escherichia coli K-12: identification of a gene encoding a novel protein involved in thiosulfate binding., J Bacteriol 172(6):3358-66

 [2] Sirko A., Hryniewicz M., Hulanicka D., Bock A., 1990, Sulfate and thiosulfate transport in Escherichia coli K-12: nucleotide sequence and expression of the cysTWAM gene cluster., J Bacteriol 172(6):3351-7

 [3] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [4] Liu H., Hou Y., Wang Y., Li Z., 2020, Enhancement of Sulfur Conversion Rate in the Production of l-Cysteine by Engineered Escherichia coli., J Agric Food Chem 68(1):250-257

 [5] Lochowska A., Iwanicka-Nowicka R., Plochocka D., Hryniewicz MM., 2001, Functional dissection of the LysR-type CysB transcriptional regulator. Regions important for DNA binding, inducer response, oligomerization, and positive control., J Biol Chem 276(3):2098-107

 [6] Lochowska A., Iwanicka-Nowicka R., Zaim J., Witkowska-Zimny M., Bolewska K., Hryniewicz MM., 2004, Identification of activating region (AR) of Escherichia coli LysR-type transcription factor CysB and CysB contact site on RNA polymerase alpha subunit at the cysP promoter., Mol Microbiol 53(3):791-806

 [7] White-Ziegler CA., Malhowski AJ., Young S., 2007, Human body temperature (37degrees C) increases the expression of iron, carbohydrate, and amino acid utilization genes in Escherichia coli K-12., J Bacteriol 189(15):5429-40

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