RegulonDB RegulonDB 10.9: Operon Form
   

cysDNC operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: cysDNC
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: cysDNC
Synonym(s): cysD, cysNC
Gene(s): cysC, cysN, cysD   Genome Browser M3D Gene expression COLOMBOS
Note(s): Originally, there were two operons, cysNC and cysD, described. In Leyh TS,1988, it was demonstrated that cysN and cysC were both contained in one operon. The evidence was the strong polarity between cysN and cysC, although no promoter was described. It was not determined whether the upstream gene (cysD) was part of the same operon, but the authors suggested that all the genes could be transcribed because their products are part of the same metabolic pathway. In a more recent paper, Malo MS,1990, a promoter upstream of cysD was described. In a third reference, Leyh TS,1992, it is proposed that the three genes are translationally coupled, confirming that they reside within the same operon. Additionally, a terminator of transcription has also been identified.
Evidence: [BTEI] Boundaries of transcription experimentally identified
[IEP] Inferred from expression pattern
[ITCR] Inferred through co-regulation
[PM] Polar mutation
Reference(s): [1] Leyh TS., et al., 1988
[2] Leyh TS., et al., 1992
[3] Malo MS., et al., 1990
Promoter
Name: cysDp
+1: 2876368
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 39
Sequence: aactactcattctaattggtaatttcattcgttctcttacgctccctatagtcgaaacatCtgatggcaagaaaatagcgg
                        -35                    -10          +1                   
Note(s): Originally, there were two promoters, cysNC and cysD, because there were two transcription units, cysD and cysNC in the database. With the current information, there is only one transcription unit, cysDNC, with three genes. See the note for transcription unit cysDNC.
Evidence: [HIPP]
[ICWHO]
[TIM]
Reference(s): [4] Huerta AM., et al., 2003
[3] Malo MS., et al., 1990
Terminator(s)
Type: rho-independent
Sequence: cgcaaataacCAGGAGATAAAACCGACCACGGCACCAGGCAGTGACCATGTGGTTTCTTCAtcctcagtaa
Reference(s): [2] Leyh TS., et al., 1992
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal CysB-O-acetyl-L-serine1 activator cysDp 2876432 2876473 -85.0 ccggtgccttAAGCACTTTTTGATATTAGCTTTGCCAAATCGTTATTCCGTTAaggaactact nd [BCE] nd
Note(s): 1The protein CysB regulates the expression of the cysDNC operon. This protein was previously indicated as involved in regulating the cydD and cysNC promoters. But the cysNC promoter has been eliminated from the database. See the note for the cysD promoter and the note for the transcription unit cysDNC.1The protein CysB regulates the expression of the cysDNC operon. This protein was previously indicated as involved in regulating the cydD and cysNC promoters. But the cysNC promoter has been eliminated from the database. See the note for the cysD promoter and the note for the transcription unit cysDNC.





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