|Gene(s):||fdhF Genome Browser M3D Gene expression COLOMBOS|
|Note(s):||This transcription unit is repressed and induced anaerobically. It is repressed in the presence of nitrate and induced in the presence of formate Wang H,2003
Based on qRT-PCR, it was found that three selenopolypeptide genes, fdnG, fdoG, and fdhF, were induced more than 2-fold by both 0.01 and 5 mM Na2SeO3 (sodium selenite) 24531660
 Rosentel JK., et al., 1995
 Wang H., et al., 2003
|Sigma Factor:||Sigma54 Sigmulon|
|Distance from start of the gene:||41|
-24 -12 +1
 Birkmann A., et al., 1987
 Huerta AM., et al., 2003
 Schlensog V., et al., 1994
|Reference(s):|| Bognar AL., et al., 1987|
|Type||Transcription factor||Function||Promoter||Binding Sites||Growth Conditions||Evidence (Confirmed, Strong, Weak)||Reference(s)|
|remote||FhlA-formate||activator||fdhFp||4299505||4299518||-104.5||tcgtttcgtcACGTCAAAACTGACGacagcctgtt||nd||[APIORCISFBSCS], [BPP], [GEA]|||
|remote||FhlA-formate||activator||fdhFp||4299530||4299543||-129.5||cgcgtgatggCTGTCACGCGGTATTtcgtttcgtc||nd||[APIORCISFBSCS], [BPP], [GEA]|||
|Transcription factor||Function||Promoter||Binding Sites||Evidence (Confirmed, Strong, Weak)||Reference(s)|
1Under anaerobiosis, FNR activates fdhF gene expression, but it is not known if this regulation is direct or indirect Salmon K,2003.
This regulatory interaction was determined only by microarray analysis.1NarL appears to bind two regions upstream of the fdhF promoter: one of them is between position -130 and position ?175 and the other one is between position -190 and position -27 relative to the transcription start site. NarP appears to antagonize the ability of NarL to repress fdhF gene expression.1NarP appears to be a mild antagonist of NarL's ability to repress fdhF gene expresion Wang H,2003 .3Under anaerobiosis, FNR activates fdhF gene expression, but it is not known if this regulation is direct or indirect Salmon K,2003.
This regulatory interaction was determined only by microarray analysis.
4NarP appears to be a mild antagonist of NarL's ability to repress fdhF gene expresion Wang H,2003 .
5NarL appears to bind two regions upstream of the fdhF promoter: one of them is between position -130 and position ?175 and the other one is between position -190 and position -27 relative to the transcription start site. NarP appears to antagonize the ability of NarL to repress fdhF gene expression.
|RNA cis-regulatory element|
|Regulation, transcriptional elongation|
|Structure type||Energy||LeftPos||RightPos||Sequence (RNA-strand)|
|Notes: "The provided "Sequence" is that of the RNA strand, i.e. U's are shown instead of T's and regulators on the reverse strand will appear as the reverse complement of the sequence delimited by LeftPos-RigtPos"|
|REGULATION, RNA cis-regulatory element:|
|Evidence:||[ICA] Inferred by computational analysis|
|Reference(s):|| null null|
|Description||Rfam score||Left Pos||Right Pos||Sequence (RNA-strand)|
|Selenocysteine insertion sequence 2||87.1||4298900||4298954||ugcugcgcucGUGUCUGACACGGCCCAUCGGUUGCAGGUCUGCACCAAUCGGUCGGUAAUGGCGCaaugagcaau|
|Notes: "The provied "Sequence" is that of the RNA strand, i.e. U's are shown instead of T's and regulators on the reverse strand will appears as the reverse complement of the sequence delimited by LeftPos-RightPos"|
 Rosentel JK., Healy F., Maupin-Furlow JA., Lee JH., Shanmugam KT., 1995, Molybdate and regulation of mod (molybdate transport), fdhF, and hyc (formate hydrogenlyase) operons in Escherichia coli., J Bacteriol 177(17):4857-64
 Wang H., Gunsalus RP., 2003, Coordinate regulation of the Escherichia coli formate dehydrogenase fdnGHI and fdhF genes in response to nitrate, nitrite, and formate: roles for NarL and NarP., J Bacteriol 185(17):5076-85
 Schlensog V., Lutz S., Bock A., 1994, Purification and DNA-binding properties of FHLA, the transcriptional activator of the formate hydrogenlyase system from Escherichia coli., J Biol Chem 269(30):19590-6
 Bognar AL., Osborne C., Shane B., 1987, Primary structure of the Escherichia coli folC gene and its folylpolyglutamate synthetase-dihydrofolate synthetase product and regulation of expression by an upstream gene., J Biol Chem 262(25):12337-43
 Salmon K., Hung SP., Mekjian K., Baldi P., Hatfield GW., Gunsalus RP., 2003, Global gene expression profiling in Escherichia coli K12. The effects of oxygen availability and FNR., J Biol Chem 278(32):29837-55