RegulonDB RegulonDB 10.9: Operon Form
   

folA operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: folA
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: folA
Gene(s): folA   Genome Browser M3D Gene expression COLOMBOS
Note(s): Both TyrR sites, together with the IHF site present in the folA upstream region, form a complex that is designed to respond primarily to the increased levels of tyrosine by activating the expression of folA Yang J,2007
Evidence: [BTEI] Boundaries of transcription experimentally identified
Reference(s): [1] Blanchin-Roland S., et al., 1986
Promoter
Name: folAp
+1: 49799
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 24
Sequence: ttcctcaacatcatcctcgcaccagtcgacgacggtttacgctttacgtatagtggcgacAattttttttatcgggaaatc
                         -35                     -10        +1                   
Evidence: [HIPP]
[ICWHO]
[RS-EPT-CBR]
Reference(s): [1] Blanchin-Roland S., et al., 1986
[2] Huerta AM., et al., 2003
[3] Salgado H, et al., 2012
[4] Smith DR., et al., 1982
TF binding sites (TFBSs)
[IHF,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal IHF activator folAp 49728 49740 -65.0 tcgccagcagAATATAAAATTTTCctcaacatca nd [APIORCISFBSCS], [GEA] [5]
[TyrR,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote TyrR-tyrosine activator folAp 49642 49659 -148.5 taaagagtgaCGTAAATCACACTTTACAGctaactgttt nd [APIORCISFBSCS], [BPP], [GEA], [SM] [5]
remote TyrR-tyrosine activator folAp 49684 49701 -106.0 tttgtttcatTGTAATGCGGCGAGTCCAGggagagagcg nd [APIORCISFBSCS], [BPP], [GEA], [SM] [5]


Evidence    

 [HIPP] Human inference of promoter position

 [ICWHO] Inferred computationally without human oversight

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [APIORCISFBSCS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.

 [GEA] Gene expression analysis

 [BPP] Binding of purified proteins

 [SM] Site mutation


Reference(s)    

 [1] Blanchin-Roland S., Blanquet S., Schmitter JM., Fayat G., 1986, The gene for Escherichia coli diadenosine tetraphosphatase is located immediately clockwise to folA and forms an operon with ksgA., Mol Gen Genet 205(3):515-22

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [3] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [4] Smith DR., Rood JI., Bird PI., Sneddon MK., Calvo JM., Morrison JF., 1982, Amplification and modification of dihydrofolate reductase in Escherichia coli. Nucleotide sequence of fol genes from mutationally altered plasmids., J Biol Chem 257(15):9043-8

 [5] Yang J., Ogawa Y., Camakaris H., Shimada T., Ishihama A., Pittard AJ., 2007, folA, a New Member of the TyrR Regulon in Escherichia coli K-12., J Bacteriol 189(16):6080-4

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