RegulonDB RegulonDB 11.1: Operon Form
   

manA operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: manA
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: manA
Gene(s): manA   Genome Browser M3D Gene expression COLOMBOS
Evidence: [COMP-HINF] Inferred by a human based on computational evidence
[EXP-IDA-TRANSCRIPT-LEN-DETERMINATION] Length of transcript experimentally determined
Reference(s): [1] Miles JS., et al., 1984
[2] Miles JS., et al., 1984
Promoter
Name: manAp
+1: 1688551
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 25
Sequence: cgactttttgcggctccaggttacttcccgtaggattcttgctttaatagtgggattaatTtccacattaaaacagggatt
                               -35                    -10   +1                   
Evidence: [COMP-AINF]
[EXP-IDA-TRANSCRIPTION-INIT-MAPPING]
Reference(s): [3] Huerta AM., et al., 2003
[2] Miles JS., et al., 1984
TF binding sites (TFBSs)
[CRP,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence Confidence level (C: Confirmed, S: Strong, W: Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal CRP-cyclic-AMP activator manAp 1688476 1688499 -64.0 tttttaacaaAAACTGAGACTAGTACGACTTTTTgcggctccag nd [COMP-HINF-SIMILAR-TO-CONSENSUS], [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [COMP-HINF-SIMILAR-TO-CONSENSUS] W [2], [4]
[CRP,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence Confidence level (C: Confirmed, S: Strong, W: Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal CRP-cyclic-AMP repressor manAp 1688539 1688560 -1.5 ttgctttaatAGTGGGATTAATTTCCACATTAaaacagggat nd [COMP-HINF-SIMILAR-TO-CONSENSUS], [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [COMP-HINF-SIMILAR-TO-CONSENSUS] W [1], [4]
proximal CRP-cyclic-AMP repressor manAp 1688547 1688568 7.5 atagtgggatTAATTTCCACATTAAAACAGGGattgatcatg nd [COMP-HINF-SIMILAR-TO-CONSENSUS], [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [COMP-HINF-SIMILAR-TO-CONSENSUS] W [1], [4]


RNA cis-regulatory element    
Regulation, transcriptional elongation  
Attenuator type: Translational
Strand: forward
  Structure type Energy LeftPos RightPos Sequence (RNA-strand)
  terminator -10.4 1688529 1688566 gtaggattctTGCTTTAATAGTGGGATTAATTTCCACATTAAAACAGggattgatca
Notes: "The provided "Sequence" is that of the RNA strand, i.e. U's are shown instead of T's and regulators on the reverse strand will appear as the reverse complement of the sequence delimited by LeftPos-RigtPos"


Evidence    

 [COMP-AINF] Inferred computationally without human oversight

 [EXP-IDA-TRANSCRIPTION-INIT-MAPPING] Transcription initiation mapping

 [COMP-HINF-SIMILAR-TO-CONSENSUS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.

 [EXP-IEP-GENE-EXPRESSION-ANALYSIS] Gene expression analysis


Reference(s)    

 [1] Miles JS., Guest JR., 1984, Complete nucleotide sequence of the fumarase gene fumA, of Escherichia coli., Nucleic Acids Res 12(8):3631-42

 [2] Miles JS., Guest JR., 1984, Nucleotide sequence and transcriptional start point of the phosphomannose isomerase gene (manA) of Escherichia coli., Gene 32(1-2):41-8

 [3] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [4] Surmann K., Stopp M., Worner S., Dhople VM., Volker U., Unden G., Hammer E., 2020, Fumarate dependent protein composition under aerobic and anaerobic growth conditions in Escherichia coli., J Proteomics 212:103583

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