RegulonDB RegulonDB 11.2: Operon Form
   

aspC operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: aspC
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: aspC
Gene(s): aspC   Genome Browser M3D Gene expression COLOMBOS
Note(s): Under nitrogen-rich growth conditions, the expression of the icdA gene increased in mutants for two genes that encode two terminal oxidases, cyoA and cydB, and in mutants for two transcriptional regulators, Fnr and Fur. However, under nitrogen-limited growth conditions, gene expression was decreased in the cyoA and cydB and Fnr mutants Kumar R,2011
The expression of the gene aspC is enhanced under oxygen limitation Kawaguchi H, Miyagawa H, Nakamura-Tsuruta S, Takaya N, Ogino C, Kondo A,2019.
Evidence: [COMP-AINF] Inferred computationally without human oversight
Promoter
Name: aspCp
+1: 985743
Distance from start of the gene: 34
Sequence: agcctgcgttttcatcagtaatagttggaattttgtaaatctcccgttaccctgatagcgGacttcccttctgtaaccata
Evidence: [COMP-AINF-POSITIONAL-IDENTIFICATION]
[EXP-IDA-RNA-POLYMERASE-FOOTPRINTING]
Reference(s): [1] Fotheringham IG., et al., 1986
[2] Staden R. 1984
TF binding sites (TFBSs)
[FNR,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence Confidence level (C: Confirmed, S: Strong, W: Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd FNR activator aspCp nd nd nd nd nd [EXP-IEP-GENE-EXPRESSION-ANALYSIS] W [3]
[FNR,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence Confidence level (C: Confirmed, S: Strong, W: Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd FNR repressor aspCp nd nd nd nd nd [EXP-IEP-GENE-EXPRESSION-ANALYSIS] W [3]
[Fur,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence Confidence level (C: Confirmed, S: Strong, W: Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd Fur-Fe2+ repressor aspCp nd nd nd nd nd [EXP-IEP-GENE-EXPRESSION-ANALYSIS] W [3]


Evidence    

 [COMP-AINF-POSITIONAL-IDENTIFICATION] Automated inference of promoter position

 [EXP-IDA-RNA-POLYMERASE-FOOTPRINTING] RNA polymerase footprinting

 [EXP-IEP-GENE-EXPRESSION-ANALYSIS] Gene expression analysis


Reference(s)    

 [1] Fotheringham IG., Dacey SA., Taylor PP., Smith TJ., Hunter MG., Finlay ME., Primrose SB., Parker DM., Edwards RM., 1986, The cloning and sequence analysis of the aspC and tyrB genes from Escherichia coli K12. Comparison of the primary structures of the aspartate aminotransferase and aromatic aminotransferase of E. coli with those of the pig aspartate aminotransferase isoenzymes., Biochem J 234(3):593-604

 [2] Staden R., 1984, Graphic methods to determine the function of nucleic acid sequences., Nucleic Acids Res 12(1 Pt 2):521-38

 [3] Kumar R., Shimizu K., 2011, Transcriptional regulation of main metabolic pathways of cyoA, cydB, fnr, and fur gene knockout Escherichia coli in C-limited and N-limited aerobic continuous cultures., Microb Cell Fact 10:3

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