RegulonDB RegulonDB 10.9: Operon Form
   

glnS operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: glnS
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit       
Name: glnS
Gene(s): glnS   Genome Browser M3D Gene expression COLOMBOS
Evidence: [BTEI] Boundaries of transcription experimentally identified
[LTED] Length of transcript experimentally determined
Reference(s): [1] Cheung AY., et al., 1984
[2] Nonaka G., et al., 2006
[3] Yamao F., et al., 1982
Promoter
Name: glnSp2
+1: 705999
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 94
Sequence: aagcggtggagatcttctctgccgctttttttttcatcaatcatccccataatccttgttAgattatcaattttaaaaaac
                          -35                   -10         +1                   
Evidence: [ICWHO]
[IDA]
Reference(s): [4] Huerta AM., et al., 2003
[2] Nonaka G., et al., 2006
Terminator(s)
Type: rho-independent
Sequence: caaacaatccAAAACGCCGCGTTCAGCGGCGTTTTttctgctttt
Reference(s): [1] Cheung AY., et al., 1984


Transcription unit       
Name: glnS
Gene(s): glnS   Genome Browser M3D Gene expression COLOMBOS
Evidence: [BTEI] Boundaries of transcription experimentally identified
[LTED] Length of transcript experimentally determined
Reference(s): [1] Cheung AY., et al., 1984
[2] Nonaka G., et al., 2006
[3] Yamao F., et al., 1982
Promoter
Name: glnSp
+1: 705999
Sigma Factor: Sigma32 Sigmulon
Distance from start of the gene: 94
Sequence: aagcggtggagatcttctctgccgctttttttttcatcaatcatccccataatccttgttAgattatcaattttaaaaaac
                          -35                -10            +1                   
Evidence: [ICWHO]
[IDA]
Reference(s): [4] Huerta AM., et al., 2003
[2] Nonaka G., et al., 2006
Terminator(s)
Type: rho-independent
Sequence: caaacaatccAAAACGCCGCGTTCAGCGGCGTTTTttctgctttt
Reference(s): [1] Cheung AY., et al., 1984


Transcription unit       
Name: glnS
Gene(s): glnS   Genome Browser M3D Gene expression COLOMBOS
Evidence: [BTEI] Boundaries of transcription experimentally identified
[LTED] Length of transcript experimentally determined
Reference(s): [1] Cheung AY., et al., 1984
[3] Yamao F., et al., 1982
Promoter
Name: glnSp1
+1: 706060
Distance from start of the gene: 33
Sequence: gattatcaattttaaaaaactaacagttgtcagcctgtcccgcttataagatcatacgccGttatacgttgtttacgcttt
Evidence: [IMP]
[RS-EPT-CBR]
[TIM]
Reference(s): [1] Cheung AY., et al., 1984
[5] Plumbridge J., et al., 1989
[6] Salgado H, et al., 2012
Terminator(s)
Type: rho-independent
Sequence: caaacaatccAAAACGCCGCGTTCAGCGGCGTTTTttctgctttt
Reference(s): [1] Cheung AY., et al., 1984


RNA cis-regulatory element    
Regulation, transcriptional elongation  
Attenuator type: Transcriptional
Strand: forward
Evidence: [ICA] Inferred by computational analysis
Reference(s): [7] Merino E, et al., 2005
  Structure type Energy LeftPos RightPos Sequence (RNA-strand)
  terminator -17.0 705939 705973 aacaatgacaAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCatcaatcatc
  anti-terminator -24.0 705892 705944 aaaagtaatcTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGtggagatctt
Notes: "The provided "Sequence" is that of the RNA strand, i.e. U's are shown instead of T's and regulators on the reverse strand will appear as the reverse complement of the sequence delimited by LeftPos-RigtPos"




Reference(s)    

 [1] Cheung AY., Soll D., 1984, In vivo and in vitro transcription of the Escherichia coli glutaminyl-tRNA synthetase gene., J Biol Chem 259(15):9953-8

 [2] Nonaka G., Blankschien M., Herman C., Gross CA., Rhodius VA., 2006, Regulon and promoter analysis of the E. coli heat-shock factor, sigma32, reveals a multifaceted cellular response to heat stress., Genes Dev 20(13):1776-89

 [3] Yamao F., Inokuchi H., Cheung A., Ozeki H., Soll D., 1982, Escherichia coli glutaminyl-tRNA synthetase. I. Isolation and DNA sequence of the glnS gene., J Biol Chem 257(19):11639-43

 [4] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [5] Plumbridge J., Soll D., 1989, Characterization of cis-acting mutations which increase expression of a glnS-lacZ fusion in Escherichia coli., Mol Gen Genet 216(1):113-9

 [6] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [7] Merino E, Yanofsky C., 2005, Transcription attenuation: a highly conserved regulatory strategy used by bacteria., Trends Genet. 2005 May;21(5):260-4.


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