RegulonDB RegulonDB 11.1: Operon Form
   

lpp operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: lpp
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit       
Name: lpp
Gene(s): lpp   Genome Browser M3D Gene expression COLOMBOS
Evidence: [EXP-IDA-BOUNDARIES-DEFINED] Boundaries of transcription experimentally identified
Reference(s): [1] Rhodius VA., et al., 2005
Promoter
Name: lppp2
+1: 1757296
Distance from start of the gene: 125
Sequence: tgaatccgatggaagcatcctgttttctctcaatttttttatctaaaacccagcgttcgaTgcttctttgagcgaacgatc
Note(s): The lppp2 promoter appears to be negatively regulated by σE. In agreement with this, a consensus sequence for RNA polymerase-σE binding is located upstream of this promoter, but in the opposite direction from lpp Rhodius VA,2005.
Evidence: [COMP-AINF-POSITIONAL-IDENTIFICATION]
[EXP-IDA-TRANSCRIPTION-INIT-MAPPING]
Reference(s): [1] Rhodius VA., et al., 2005


Transcription unit       
Name: lpp
Gene(s): lpp   Genome Browser M3D Gene expression COLOMBOS
Evidence: [EXP-IDA-BOUNDARIES-DEFINED] Boundaries of transcription experimentally identified
Reference(s): [2] Nakamura K., et al., 1979
Promoter
Name: lpp
+1: 1757383
Distance from start of the gene: 38
Sequence: aagtgccttcccatcaaaaaaatattctcaacataaaaaactttgtgtaatacttgtaacGctacatggagattaactcaa
Note(s): The activity of this promoter appears to be decreased in the presence of σE. It is repressed during the first 5 minutes following σE induction and decreased from 5 to 10 minutes and from 10 to 20 minutes after induction of the σ factor Lacoux C,2020.
Evidence: [EXP-IDA-TRANSCRIPTION-INIT-MAPPING]
[RS-EPT-CBR]
Reference(s): [2] Nakamura K., et al., 1979
[3] Salgado H, et al., 2012


Evidence    

 [COMP-AINF-POSITIONAL-IDENTIFICATION] Automated inference of promoter position

 [EXP-IDA-TRANSCRIPTION-INIT-MAPPING] Transcription initiation mapping

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Rhodius VA., Suh WC., Nonaka G., West J., Gross CA., 2005, Conserved and variable functions of the sigmaE stress response in related genomes., PLoS Biol 4(1):e2

 [2] Nakamura K., Inouye M., 1979, DNA sequence of the gene for the outer membrane lipoprotein of E. coli: an extremely AT-rich promoter., Cell 18(4):1109-17

 [3] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [4] Guo MS., Updegrove TB., Gogol EB., Shabalina SA., Gross CA., Storz G., 2014, MicL, a new σE-dependent sRNA, combats envelope stress by repressing synthesis of Lpp, the major outer membrane lipoprotein., Genes Dev 28(14):1620-34

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