RegulonDB RegulonDB 10.10: Operon Form
   

cstA operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: cstA
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: cstA
Synonym(s): cst-2
Gene(s): cstA   Genome Browser M3D Gene expression COLOMBOS
Evidence: [BTEI] Boundaries of transcription experimentally identified
Reference(s): [1] Schultz JE., et al., 1991
Promoter
Name: cstAp1
+1: 629855
Distance from start of the gene: 39
Sequence: tcaactccgatttacatggttgctgtgttgttaaattgtacaaagatgttatagaaacaaAatgtaacatctctatggaca
Evidence: [TIM]
Reference(s): [1] Schultz JE., et al., 1991
Terminator(s)
Type: rho-independent
Sequence: ttagtgcccaGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTgaccgagcgc
Reference(s): [1] Schultz JE., et al., 1991
TF binding sites (TFBSs)
[CRP,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal CRP-cyclic-AMP activator cstAp1 629755 629776 -89.5 actcggttaaCGGAGTGATCGAGTTAACATTGttaagttaaa nd [GEA], [SM] [1], [2], [3]


Transcription unit       
Name: cstA
Synonym(s): cst-2
Gene(s): cstA   Genome Browser M3D Gene expression COLOMBOS
Evidence: [BTEI] Boundaries of transcription experimentally identified
Reference(s): [1] Schultz JE., et al., 1991
Promoter
Name: cstAp2
+1: 629864
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 30
Sequence: atttacatggttgctgtgttgttaaattgtacaaagatgttatagaaacaaaatgtaacaTctctatggacacgcacacgg
                      -35                        -10        +1                   
Evidence: [ICWHO]
[TIM]
Reference(s): [4] Huerta AM., et al., 2003
[1] Schultz JE., et al., 1991
Terminator(s)
Type: rho-independent
Sequence: ttagtgcccaGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTgaccgagcgc
Reference(s): [1] Schultz JE., et al., 1991


Transcription unit       
Name: cstA
Synonym(s): cst-2
Gene(s): cstA   Genome Browser M3D Gene expression COLOMBOS
Evidence: [BTEI] Boundaries of transcription experimentally identified
Reference(s): [1] Schultz JE., et al., 1991
Promoter
Name: cstAp3
+1: 629873
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 21
Sequence: gttgctgtgttgttaaattgtacaaagatgttatagaaacaaaatgtaacatctctatggAcacgcacacggataacaact
                    -35                        -10          +1                   
Evidence: [ICWHO]
[TIM]
Reference(s): [4] Huerta AM., et al., 2003
[1] Schultz JE., et al., 1991
Terminator(s)
Type: rho-independent
Sequence: ttagtgcccaGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTgaccgagcgc
Reference(s): [1] Schultz JE., et al., 1991


Evidence    

 [TIM] Transcription initiation mapping

 [GEA] Gene expression analysis

 [SM] Site mutation

 [ICWHO] Inferred computationally without human oversight


Reference(s)    

 [1] Schultz JE., Matin A., 1991, Molecular and functional characterization of a carbon starvation gene of Escherichia coli., J Mol Biol 218(1):129-40

 [2] Blum PH., Jovanovich SB., McCann MP., Schultz JE., Lesley SA., Burgess RR., Matin A., 1990, Cloning and in vivo and in vitro regulation of cyclic AMP-dependent carbon starvation genes from Escherichia coli., J Bacteriol 172(7):3813-20

 [3] Dubey AK., Baker CS., Suzuki K., Jones AD., Pandit P., Romeo T., Babitzke P., 2003, CsrA regulates translation of the Escherichia coli carbon starvation gene, cstA, by blocking ribosome access to the cstA transcript., J Bacteriol 185(15):4450-60

 [4] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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