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| Operon |  |
|---|---|
| Name: | srlAEBD-gutM-srlR-gutQ |
| This page displays every known transcription unit of this operon and their known regulation. | |
| Name: | srlAEBD-gutM-srlR-gutQ |
| Synonym(s): | OP00177, gutABDMR, srlABDMR |
| Gene(s): | srlA, srlE, srlB, srlD, gutM, srlR, gutQ Genome Browser M3D Gene expression COLOMBOS |
| Evidence: | [LTED] Length of transcript experimentally determined |
| Reference(s): |
[1] McEntee K. 1977 [2] Yamada M., et al., 1988 [3] Yamada M., et al., 1987 |
| Promoter | |
| Name: | srlAp |
| +1: | 2825791 |
| Sigma Factor: | Sigma70 Sigmulon |
| Distance from start of the gene: | 41 |
| Sequence: |
atctttcattttgcgatcaaaataacacttttaaatctttcaatctgattagattaggttGccgtttggtaataaaacaat -35 -10 +1 |
| Note(s): | Based on the one-round PCR product method, a novel site-directed mutagenesis method, it was determined that an srlA-driven expression system is a tool applicable for strong expression of foreign genes under cultivation conditions in LB medium without induction 30269209 In addition, it was determined that the sequence adjacent to the start codon of the srlA gene is essential for high expression and that its promoter region is 100 bp. srlAp is a constitutive promoter in LB medium, and it is a sorbitol-enhanceable and glucose-, fructose-, galactose-, mannose-, xylose-, and arabinose- repressible promoter 30269209 |
| Evidence: | [TIM] |
| Reference(s): | [2] Yamada M., et al., 1988 |
| Type | Transcription factor | Function | Promoter | Binding Sites | Growth Conditions | Evidence (Confirmed, Strong, Weak) | Reference(s) | |||
|---|---|---|---|---|---|---|---|---|---|---|
| LeftPos | RightPos | Central Rel-Pos | Sequence | |||||||
| remote | CRP-cAMP | activator | srlAp | 2825438 | 2825459 | -342.0 | atctgcatcaCATTGTGCTGCGGCTCAAGCAGgaagccgcca | nd | [AIBSCS], [CV(GEA/ROMA)], [GEA] | [2], [4] |
| proximal | CRP-cAMP | activator | srlAp | 2825739 | 2825760 | -41.5 | ttatctttcaTTTTGCGATCAAAATAACACTTttaaatcttt | nd | [AIBSCS], [CV(GEA/ROMA)], [GEA] | [2], [4] |
| Name: | srlR |
| Synonym(s): | gutR |
| Gene(s): | srlR Genome Browser M3D Gene expression COLOMBOS |
| Reference(s): | [2] Yamada M., et al., 1988 |
| Promoter | |
| Name: | srlRp |
| +1: | 2828860 |
| Distance from start of the gene: | 187 |
| Sequence: |
cgatcagcagcgcatcgtcgacaccttgtttatgaaaggactgaccgtcttcgcccgaccGcaaaaaattcccgcaattac |
| Note(s): | The srlR gene has its own promoter, but expression of this gene is primarily due to readthrough from the slr operon. The putative srlR promoter sequence may be TCTTCG, as the -10 sequence and TTGTTT as the -35 sequence. |
| Evidence: |
[HIPP] [IMP] [TIM] |
| Reference(s): | [2] Yamada M., et al., 1988 |
| Regulation by sRNA | |
|---|
| Small RNA name (Regulator) | Regulation type | Mechanism | Function | Binding Sites | Evidence | Reference | |||
|---|---|---|---|---|---|---|---|---|---|
| LeftPos | RightPos | Sequence (RNA-strand) | |||||||
| gcvB | unknown | unknown | repressor | [IMP] | [1] [6] |
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| gcvB | unknown | unknown | repressor | [IMP] | [6] | ||||
| Notes: "The provided sequence is that of the RNA strand,i.e. 'U's are showed instead the 'T'" |
| Reference(s) |
|
|---|---|
[TIM] Transcription initiation mapping