RegulonDB RegulonDB 10.8: Operon Form
   

alkA operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: alkA
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: alkA
Synonym(s): OP00002
Gene(s): alkA   Genome Browser M3D Gene expression COLOMBOS
Note(s): Both the unmethylated and methylated forms of the Ada protein can activate alkA transcription in vivo and in vitro Landini P,1999
Evidence: [AISDTU] Automated inference that a single-gene directon is a transcription unit
Reference(s): [1] Nakabeppu Y., et al., 1984
[2] Volkert MR. 1988
Promoter
Name: alkAp
+1: 2147559
Sigma Factor: Sigma38, Sigma70
Distance from start of the gene: 19
Sequence: tcgcgacaaccggaatatgaaagcaaagcgcagcgtctgaataacgtttatgctgaaagcGgatgaataaggagatgcgat
                      -35                        -10        +1                   
Note(s): σ70 is the sigma factor principally responsible for the alkA transcription. However alkA is highly dependent on alternative factor σ38 (RpoS) during the stationary phase. In contrast, the transcription factor Ada in its methylated state negatively regulates σ38-dependent transcription Landini P,1999.
EσS is more efficient than Eσ70 in carrying out transcription from the alkA promoter in the absence of Ada (Ada is a repressor for EσS) Landini P,1999
Evidence: [CV(RPF/RS-EPT-CBR)]
[CV(RPF/TA)]
[CV(RS-EPT-CBR/TA)]
[RPF]
[RS-EPT-CBR]
Reference(s): [3] Lai CF., et al., 1999
[4] Landini P., et al., 1999
[5] Salgado H, et al., 2012
TF binding sites (TFBSs)
[Ada,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal Ada1 activator alkAp 2147589 2147613 -41.5 gccgtcgcgaCAACCGGAATATGAAAGCAAAGCGCagcgtctgaa nd [APIORCISFBSCS], [BPP], [CV(CHIP-SV/GEA/ROMA)], [CV(CHIP-SV/SM)], [CV(GEA/ROMA)], [CV(GEA/ROMA/SM)], [GEA], [SM] [4], [6], [7], [8], [9], [10], [11], [12]
Note(s): 1Ada activates alkA transcription via direct interaction with σ70 region 4 Landini P,19991Ada activates alkA transcription via direct interaction with σ70 region 4 Landini P,1999


RNA cis-regulatory element    
Regulation, transcriptional elongation  
Attenuator type: Transcriptional
Strand: reverse
  Structure type Energy LeftPos RightPos Sequence (RNA-strand)
  terminator -9.3 2147747 2147780 gcaaagcattGAAGGCAGCAGCGTGCTGTCGTTTTCCATTTTTagcaaatgcg
  anti-terminator -12.9 2147768 2147815 tacagccattCGCTTACCGCTTCACGCGTTGGCGCGCAAAGCATTGAAGGCAGCAGCgtgctgtcgt
  anti-anti-terminator -8.0 2147813 2147841 cgtctgccggAACATCATGATGGCGGTACAGCCATTCGcttaccgctt
Notes: "The provided "Sequence" is that of the RNA strand, i.e. U's are shown instead of T's and regulators on the reverse strand will appear as the reverse complement of the sequence delimited by LeftPos-RigtPos"


Evidence    

 [CV(RPF/RS-EPT-CBR)] cross validation(RPF/RS-EPT-CBR)

 [CV(RPF/TA)] cross validation(RPF/TA)

 [CV(RS-EPT-CBR/TA)] cross validation(RS-EPT-CBR/TA)

 [RPF] RNA polymerase footprinting

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [APIORCISFBSCS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.

 [BPP] Binding of purified proteins

 [CV(CHIP-SV/GEA/ROMA)] cross validation(CHIP-SV/GEA/ROMA)

 [CV(CHIP-SV/SM)] cross validation(CHIP-SV/SM)

 [CV(GEA/ROMA)] cross validation(GEA/ROMA)

 [CV(GEA/ROMA/SM)] cross validation(GEA/ROMA/SM)

 [GEA] Gene expression analysis

 [SM] Site mutation


Reference(s)    

 [1] Nakabeppu Y., Miyata T., Kondo H., Iwanaga S., Sekiguchi M., 1984, Structure and expression of the alkA gene of Escherichia coli involved in adaptive response to alkylating agents., J Biol Chem 259(22):13730-6

 [2] Volkert MR., 1988, Adaptive response of Escherichia coli to alkylation damage., Environ Mol Mutagen 11(2):241-55

 [3] Lai CF., Ripperger J., Wang Y., Kim H., Hawley RB., Baumann H., 1999, The STAT3-independent signaling pathway by glycoprotein 130 in hepatic cells., J Biol Chem 274(12):7793-802

 [4] Landini P., Busby SJ., 1999, The Escherichia coli Ada protein can interact with two distinct determinants in the sigma70 subunit of RNA polymerase according to promoter architecture: identification of the target of Ada activation at the alkA promoter., J Bacteriol 181(5):1524-9

 [5] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [6] Furuichi M., Yu CG., Anai M., Sakumi K., Sekiguchi M., 1992, Regulatory elements for expression of the alkA gene in response to alkylating agents., Mol Gen Genet 236(1):25-32

 [7] Landini P., Busby SJ., 1999, Expression of the Escherichia coli ada regulon in stationary phase: evidence for rpoS-dependent negative regulation of alkA transcription., J Bacteriol 181(21):6836-9

 [8] Landini P., Gaal T., Ross W., Volkert MR., 1997, The RNA polymerase alpha subunit carboxyl-terminal domain is required for both basal and activated transcription from the alkA promoter., J Biol Chem 272(25):15914-9

 [9] Saget BM., Shevell DE., Walker GC., 1995, Alteration of lysine 178 in the hinge region of the Escherichia coli ada protein interferes with activation of ada, but not alkA, transcription., J Bacteriol 177(5):1268-74

 [10] Saget BM., Walker GC., 1994, The Ada protein acts as both a positive and a negative modulator of Escherichia coli's response to methylating agents., Proc Natl Acad Sci U S A 91(21):9730-4

 [11] Sakumi K., Sekiguchi M., 1989, Regulation of expression of the ada gene controlling the adaptive response. Interactions with the ada promoter of the Ada protein and RNA polymerase., J Mol Biol 205(2):373-85

 [12] Teo I., Sedgwick B., Kilpatrick MW., McCarthy TV., Lindahl T., 1986, The intracellular signal for induction of resistance to alkylating agents in E. coli., Cell 45(2):315-24

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