Name: |
nrdDG |
Gene(s): |
nrdG, nrdD
Genome Browser
M3D Gene expression
COLOMBOS
|
Note(s): |
FNR is responsible for anaerobic activation of the nrdDG operon. Both sites of FNR (-64.5 and -33.5) have an additive effect in coordinating nrdDG operon expression in response to shifting oxygen concentrations Roca I,2008 The nrdD gene is upregulated by long-term (8 to 12 h) exposure of E. coli to some biocides Merchel Piovesan Pereira B, Wang X, Tagkopoulos I,2020.
H-NS represses expression of the class Ia (nrdAB operon) and class III (nrdDG operon) ribonucleotide reductases (RNRs) both in aerobically and in anaerobically growing cells. Under aerobic conditions, repression occurs during the exponential phase of growth as well as at the transition from the exponential to the stationary phase, a period when no dNTPs are needed. Under anoxic conditions, repression occurs mainly in exponentially growing cells Cendra Mdel M,2013
|
Evidence: |
[ITCR] Inferred through co-regulation |
Reference(s): |
[1] Garriga X., et al., 1996
|
Promoter |
|
Name: |
nrdDp |
+1: |
4462838 |
Sigma Factor: |
Sigma70 Sigmulon |
Distance from start of the gene: |
178
|
Sequence: |
caaaaaaagctcaaacatccttgatgcaaagcactatatatagactttaaaatgcgtcccAacccaatatgttgtattaat -35 -10 +1
|
Evidence: |
[ICWHO] [TIM]
|
Reference(s): |
[2] Boston T., et al., 2003 [3] Huerta AM., et al., 2003
|
Type |
Transcription factor |
Function |
Promoter |
Binding Sites |
Growth Conditions |
Evidence (Confirmed, Strong, Weak) |
Reference(s) |
LeftPos |
RightPos |
Central Rel-Pos |
Sequence |
proximal |
FNR
|
activator |
nrdDp |
4462865
|
4462878
|
-33.5
|
tcaaacatccTTGATGCAAAGCACtatatataga |
nd
|
[GEA], [APIORCISFBSCS], [BPP], [SM]
|
[2], [4]
|
proximal |
FNR
|
activator |
nrdDp |
4462896
|
4462909
|
-64.5
|
gctttttactTTGAGCTACATCAAaaaaagctca |
nd
|
[GEA], [APIORCISFBSCS], [BPP], [SM]
|
[2], [4]
|
Transcription factor |
Function |
Promoter |
Binding Sites |
Evidence (Confirmed, Strong, Weak) |
Reference(s) |
LeftPos |
RightPos |
Central Rel-Pos |
Sequence |
FNR |
activator |
nrdDp |
4462896
|
4462909
|
-64.5
|
gctttttactTTGAGCTACATCAAaaaaagctca
|
[GEA]
|
[2], [4]
|
FNR |
activator |
nrdDp |
4462865
|
4462878
|
-33.5
|
tcaaacatccTTGATGCAAAGCACtatatataga
|
[GEA]
|
[2], [4]
|
NrdR-Zn2+ |
repressor |
nrdDp |
4462853
|
4462868
|
-22.5
|
ttgatgcaaaGCACTATATATAGACTttaaaatgcg
|
[GEA]
|
[5]
|
NrdR-Zn2+ |
repressor |
nrdDp |
4462822
|
4462837
|
9.5
|
atgcgtcccaACCCAATATGTTGTATtaatcgacta
|
[GEA]
|
[5]
|
H-NS |
repressor |
nrdDp |
nd
|
nd
|
nd
|
nd
|
[GEA]
|
[6]
|