|Gene(s):||argG Genome Browser M3D Gene expression COLOMBOS|
|Note(s):||It has been suggested that a DNA loop is formed when ArgRbinds to the three Arg boxes. The binding of each of two regulators (CRP and ArgR) to the regulatory region of the argG gene interferes with the binding of each of the regulators, but ArgR binding overrides CRP binding.
CRP binds in just one site, activates argG gene expression, and represses the expression of the divergent operon metY-yhbC-nusA-infB.
argG gene expression is downregulated upon L-valine addition (isoleucine starvation) in the cellular growth medium Gummesson B, Shah SA, Borum AS, Fessler M, Mitarai N, Sørensen MA, Svenningsen SL,2020.
Based on DNA microarray analysis, the mechanism of bacterial inactivation by carvacrol and citral was studied Chueca B, Pérez-Sáez E, Pagán R, García-Gonzalo D,2017. Treatment by both compounds caused membrane damage and activated metabolism through the production of nucleotides required for DNA and RNA synthesis and metabolic processes Chueca B, Pérez-Sáez E, Pagán R, García-Gonzalo D,2017. A total of 76 and 156 genes demonstrated significant transcriptional differences by carvacrol and citral, respectively. Genes upregulated by carvacrol treatment included the multidrug efflux pump genes acrA and mdtM, genes related to the phage shock response, pspA, pspB, pspC, pspD, pspF, and pspG, and genes whose products are important for biosynthesis of arginine (argC, argG, artJ) and purine nucleotides (purC, purM). Genes upregulated by citral treatment included purH, pyrB, and pyrI. On the other hand, mutations in several differentially expressed genes confirmed the roles of ygaV, yjbO, pspC, sdhA, yejG, and ygaV in mechanisms of inactivation by carvacrol and citral Chueca B, Pérez-Sáez E, Pagán R, García-Gonzalo D,2017.
|Evidence:||[AISGDTU] Automated inference that a single-gene directon is a transcription unit|
|Sigma Factor:||Sigma70 Sigmulon|
|Distance from start of the gene:||75|
-35 -10 +1
 Huerta AM., et al., 2003
 Krin E., et al., 2003
|Type||Transcription factor||Function||Promoter||Binding Sites||Growth Conditions||Evidence (Confirmed, Strong, Weak)||Reference(s)|
|remote||ArgR-L-arginine||repressor||argGp||3318428||3318445||-125.5||ttgctaatcaTGTGAATGAATATCCAGTtcactttcat||nd||[GEA], [AIBSCS], [APIORCISFBSCS], [BPP]||, , |
|proximal||ArgR-L-arginine||repressor||argGp||3318547||3318564||-6.5||ataaaagatgATTAAATGAAAACTCATTtattttgcat||nd||[GEA], [AIBSCS], [APIORCISFBSCS], [BPP]||, , |
|proximal||ArgR-L-arginine||repressor||argGp||3318568||3318585||15.5||actcatttatTTTGCATAAAAATTCAGTgagagcggaa||nd||[GEA], [AIBSCS], [APIORCISFBSCS], [BPP]||, , |
|Transcription factor||Function||Promoter||Binding Sites||Evidence (Confirmed, Strong, Weak)||Reference(s)|
|ArgR-L-arginine||repressor||argGp||3318428||3318445||-125.5||ttgctaatcaTGTGAATGAATATCCAGTtcactttcat||[GEA]||, , |
|ArgR-L-arginine||repressor||argGp||3318547||3318564||-6.5||ataaaagatgATTAAATGAAAACTCATTtattttgcat||[GEA]||, , |
|ArgR-L-arginine||repressor||argGp||3318568||3318585||15.5||actcatttatTTTGCATAAAAATTCAGTgagagcggaa||[GEA]||, , |
 Krin E., Laurent-Winter C., Bertin PN., Danchin A., Kolb A., 2003, Transcription regulation coupling of the divergent argG and metY promoters in Escherichia coli K-12., J Bacteriol 185(10):3139-46
 Caldara M., Charlier D., Cunin R., 2006, The arginine regulon of Escherichia coli: whole-system transcriptome analysis discovers new genes and provides an integrated view of arginine regulation., Microbiology 152(Pt 11):3343-54
 Charlier D., Roovers M., Van Vliet F., Boyen A., Cunin R., Nakamura Y., Glansdorff N., Pierard A., 1992, Arginine regulon of Escherichia coli K-12. A study of repressor-operator interactions and of in vitro binding affinities versus in vivo repression., J Mol Biol 226(2):367-86