RegulonDB RegulonDB 10.9: Operon Form
   

hemL operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: hemL
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit       
Name: hemL
Gene(s): hemL   Genome Browser M3D Gene expression COLOMBOS
Evidence: [AISGDTU] Automated inference that a single-gene directon is a transcription unit
Promoter
Name: hemLp1
+1: 174902
Sigma Factor: Sigma28 Sigmulon
Distance from start of the gene: 20
Sequence: gatgtttgacgagtatttaacttgttatgaataacatagaatagcagccattcactttttCtacgaatcaggaaccctcca
                            -35                -10          +1                   
Note(s): The sequence of this promoter has been defined, by computational analyses, from the consensus sequence of the σ28F) promoters: TAAAGTTT in the -35 box and GCCGATAA in the -10 box Yu HH,2006. Thus, we assigned a putative transcription start site for this promoter based on the observation that the majority of the σ28 promoters, determined experimentally, present an important -10 motif (5'-CGA-3') at the positions -12 to -10 and a distance of 6 nucleotides between the transcription start site and the -10 box 16855242. Yu HH,2006. Park K,2001.
Evidence: [HIPP]
[ICWHO]
[IEP]
Reference(s): [1] Huerta AM., et al., 2003
[2] Zhao K., et al., 2007


Transcription unit          
Name: hemL
Gene(s): hemL   Genome Browser M3D Gene expression COLOMBOS
Evidence: [AISGDTU] Automated inference that a single-gene directon is a transcription unit
Promoter
Name: hemLp
+1: 174921
Sigma Factor: Sigma28 Sigmulon
Distance from start of the gene: 39
Sequence: gcaccagtacaagcagcctgatgtttgacgagtatttaacttgttatgaataacatagaaTagcagccattcactttttct
                          -35                 -10           +1                   
Evidence: [ICWHO]
[TIM]
Reference(s): [1] Huerta AM., et al., 2003
[3] Minagawa S., et al., 2003
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote PdhR repressor hemLp 175078 175094 -165.0 caatgatcacTTATTGGTCATACAAATAagatgacggc nd [AIBSCS] [4]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal PhoP-Phosphorylated activator hemLp 174944 174960 -31.0 aagcagcctgATGTTTGACGAGTATTTAacttgttatg nd [APIORCISFBSCS], [BPP], [GEA] [3]


Transcription unit       
Name: hemL
Gene(s): hemL   Genome Browser M3D Gene expression COLOMBOS
Evidence: [AISGDTU] Automated inference that a single-gene directon is a transcription unit
Promoter
Name: hemLp2
+1: 174941
Sigma Factor: Sigma28 Sigmulon
Distance from start of the gene: 59
Sequence: tactctaagaaatcaggattgcaccagtacaagcagcctgatgtttgacgagtatttaacTtgttatgaataacatagaat
                            -35                -10          +1                   
Note(s): The sequence of this promoter has been defined, by computational analyses, from the consensus sequence of the σ28F) promoters: TAAAGTTT in the -35 box and GCCGATAA in the -10 box Yu HH,2006. Thus, we assigned a putative transcription start site for this promoter based on the observation that the majority of the σ28 promoters, determined experimentally, present an important -10 motif (5'-CGA-3') at the positions -12 to -10 and a distance of 6 nucleotides between the transcription start site and the -10 box 16855242. Yu HH,2006. Park K,2001.
Evidence: [HIPP]
[ICWHO]
Reference(s): [1] Huerta AM., et al., 2003
[2] Zhao K., et al., 2007





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