RegulonDB RegulonDB 10.9: Operon Form
   

pagP operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: pagP
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: pagP
Synonym(s): crcA
Gene(s): pagP   Genome Browser M3D Gene expression COLOMBOS
Note(s): pagP is induced by increases in osmolarity in mature bioifilms in an RcsB-dependent manner and it also requires the GadE auxiliary regulator; its induction is not observed under planktonic conditions. Formation of a RcsB-GadE complex occurs independently of the RcsB phosphorylation cascade 30131361
Based on gene expression analysis, it was shown that pagP expression is repressed by H-NS 30131361
SlyA, a DNA-binding protein that inhibits H-NS activity, activates pagP transcription in mature biofilms Klitgaard RN,2018
The expression of the pagP gene appears to be indirectly regulated by the transcription factor PhoP in avian pathogenic E. coli (APEC) Song X,2019.
Promoter
Name: pagPp
+1: 656526
Distance from start of the gene: 31
Sequence: attattctttatgttgggtctattaaggttatgttaattgtagctttgctatgctagtagTagatttttgataaatgtttt
Evidence: [TIM]
Reference(s): [1] Eguchi Y., et al., 2004
TF binding sites (TFBSs)
[PhoP,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal PhoP-Phosphorylated activator pagPp 656486 656502 -32.0 atgttgggtcTATTAAGGTTATGTTAATtgtagctttg nd [APIORCISFBSCS], [GEA] [1]
[SlyA,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote SlyA activator pagPp 656417 656416 -109.5 aatcgatgcgCggaaatattt nd [GEA], [MCIBSPD] [2]
proximal SlyA activator pagPp 656431 656444 -89.0 aatatttaacTTGAACAAGCGGAAataaatagag nd [GEA], [MCIBSPD] [2]
proximal SlyA activator pagPp 656510 656523 -10.0 taattgtagcTTTGCTATGCTAGTagtagatttt nd [GEA], [MCIBSPD] [2]


Evidence    

 [TIM] Transcription initiation mapping

 [APIORCISFBSCS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.

 [GEA] Gene expression analysis

 [MCIBSPD] Manually curated inference based on sequence pattern discovery


Reference(s)    

 [1] Eguchi Y., Okada T., Minagawa S., Oshima T., Mori H., Yamamoto K., Ishihama A., Utsumi R., 2004, Signal transduction cascade between EvgA/EvgS and PhoP/PhoQ two-component systems of Escherichia coli., J Bacteriol 186(10):3006-14

 [2] Klitgaard RN., Jana B., Guardabassi L., Nielsen KL., Lobner-Olesen A., 2018, DNA Damage Repair and Drug Efflux as Potential Targets for Reversing Low or Intermediate Ciprofloxacin Resistance in E. coli K-12., Front Microbiol 9:1438

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