RegulonDB RegulonDB 10.9: Operon Form
   

dhaR operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: dhaR
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: dhaR
Gene(s): dhaR   Genome Browser M3D Gene expression COLOMBOS
Evidence: [AISGDTU] Automated inference that a single-gene directon is a transcription unit
Reference(s): [1] Bachler C., et al., 2005
Promoter
Name: dhaRp
+1: 1251003
Sigma Factor: Sigma38 Sigmulon
Distance from start of the gene: 63
Sequence: tagcccaaattatgtttcatagtgaaacatatgctttaatgaatgttccatattgaaactTttacgtgtattaatacttaa
                        -35                 -10             +1                   
Evidence: [AIPP]
[ICWHO]
Reference(s): [2] Gama-Castro S., et al., 2008
[3] Huerta AM., et al., 2003
TF binding sites (TFBSs)
[DhaR,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd DhaR1 repressor dhaRp nd nd nd nd nd [BPP], [GEA], [IGI] [1]
Note(s): 1Between the dhaKLM operon and the dhaR gene there is a 190-bp-long intergenic region that divergently transcribes them. DhaR is an (auto)repressor of the dhaR gene and an activator of the dhaKLM operon Bachler C,2005.1Between the dhaKLM operon and the dhaR gene there is a 190-bp-long intergenic region that divergently transcribes them. DhaR is an (auto)repressor of the dhaR gene and an activator of the dhaKLM operon Bachler C,2005.


Evidence    

 [AIPP] Automated inference of promoter position

 [ICWHO] Inferred computationally without human oversight

 [BPP] Binding of purified proteins

 [GEA] Gene expression analysis

 [IGI] Inferred from genetic interaction


Reference(s)    

 [1] Bachler C., Schneider P., Bahler P., Lustig A., Erni B., 2005, Escherichia coli dihydroxyacetone kinase controls gene expression by binding to transcription factor DhaR., EMBO J 24(2):283-93

 [2] Gama-Castro S., Jimenez-Jacinto V., Peralta-Gil M., Santos-Zavaleta A., Penaloza-Spinola MI., Contreras-Moreira B., Segura-Salazar J., Muniz-Rascado L., Martinez-Flores I., Salgado H., Bonavides-Martinez C., Abreu-Goodger C., Rodriguez-Penagos C., Miranda-Rios J., Morett E., Merino E., Huerta AM., Trevino-Quintanilla L., Collado-Vides J., 2008, RegulonDB (version 6.0): gene regulation model of Escherichia coli K-12 beyond transcription, active (experimental) annotated promoters and Textpresso navigation., Nucleic Acids Res 36(Database issue):D120-4

 [3] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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