RegulonDB RegulonDB 10.9: Operon Form
   

mntH operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: mntH
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: mntH
Gene(s): mntH   Genome Browser M3D Gene expression COLOMBOS
Note(s): MntS makes manganese more available in cells, promoting the ability of manganese to repress mntH transcription by altering cellular manganese pools and global availability, which would perturb MntR and/or Fur activity Waters LS,2011
Evidence: [AISGDTU] Automated inference that a single-gene directon is a transcription unit
Promoter
Name: mntHp
+1: 2512734
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 28
Sequence: tcaccacaatccatttaaatgaatttgataatcattctcgtttggcatagcatgaaacatAgcaaaggctatgttttagag
                         -35                    -10         +1                   
Evidence: [HIPP]
[TIM]
Reference(s): [1] Kehres DG., et al., 2002
[2] Waters LS., et al., 2011
TF binding sites (TFBSs)
[Fur,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal Fur-Fe2+1 repressor mntHp 2512757 2512775 -31.5 tccatttaaaTGAATTTGATAATCATTCTcgtttggcat nd [AIBSCS], [APIORCISFBSCS], [IGI] [1], [5]
[MntR,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal MntR-Mn2+ repressor mntHp 2512719 2512740 5.5 ggcatagcatGAAACATAGCAAAGGCTATGTTTtagaggcaca nd [AIBSCS], [BPP], [GEA] [1], [5]
[OxyR,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal OxyR activator mntHp 2512780 2512796 -54.0 tctatcagagAAATCACCACAATCCATTtaaatgaatt [AIBSCS], [GEA], [SM] [1], [3]
proximal OxyR activator mntHp 2512802 2512818 -76.0 cttttcgtagTCATAATCCTGGTCTATCagagaaatca [APIORCISFBSCS], [CEEUMA], [GEA], [IHBCE], [RSE], [SM] [1], [3], [4]
Note(s): 1Iron represses mntH expression. This repression is mediated by Fur, a transcriptional regulator that binds 59 nt upstream of the mntH gene start Patzer SI,2001.3Iron represses mntH expression. This repression is mediated by Fur, a transcriptional regulator that binds 59 nt upstream of the mntH gene start Patzer SI,2001.


RNA cis-regulatory element    
Regulation, transcriptional elongation  
Attenuator type: Translational
Strand: reverse
  Structure type Energy LeftPos RightPos Sequence (RNA-strand)
  terminator -15.1 2512708 2512753 tcattctcgtTTGGCATAGCATGAAACATAGCAAAGGCTATGTTTTAGAGGCACAagatgacgaa
Notes: "The provided "Sequence" is that of the RNA strand, i.e. U's are shown instead of T's and regulators on the reverse strand will appear as the reverse complement of the sequence delimited by LeftPos-RigtPos"


Evidence    

 [HIPP] Human inference of promoter position

 [TIM] Transcription initiation mapping

 [AIBSCS] Automated inference based on similarity to consensus sequences

 [APIORCISFBSCS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.

 [IGI] Inferred from genetic interaction

 [BPP] Binding of purified proteins

 [GEA] Gene expression analysis

 [SM] Site mutation

 [CEEUMA] ChIP-exo evidence used in manual assertion

 [IHBCE] Inferred by a human based on computational evidence

 [RSE] RNA-seq evidence


Reference(s)    

 [1] Kehres DG., Janakiraman A., Slauch JM., Maguire ME., 2002, Regulation of Salmonella enterica serovar Typhimurium mntH transcription by H(2)O(2), Fe(2+), and Mn(2+)., J Bacteriol 184(12):3151-8

 [2] Waters LS., Sandoval M., Storz G., 2011, The Escherichia coli MntR miniregulon includes genes encoding a small protein and an efflux pump required for manganese homeostasis., J Bacteriol 193(21):5887-97

 [3] Anjem A., Varghese S., Imlay JA., 2009, Manganese import is a key element of the OxyR response to hydrogen peroxide in Escherichia coli., Mol Microbiol 72(4):844-58

 [4] Seo SW., Kim D., Szubin R., Palsson BO., 2015, Genome-wide Reconstruction of OxyR and SoxRS Transcriptional Regulatory Networks under Oxidative Stress in Escherichia coli K-12 MG1655., Cell Rep 12(8):1289-99

 [5] Patzer SI., Hantke K., 2001, Dual repression by Fe(2+)-Fur and Mn(2+)-MntR of the mntH gene, encoding an NRAMP-like Mn(2+) transporter in Escherichia coli., J Bacteriol 183(16):4806-13

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