RegulonDB RegulonDB 11.1: Operon Form
   

recN operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: recN
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: recN
Gene(s): recN   Genome Browser M3D Gene expression COLOMBOS
Note(s): The recN gene is repressed by both DnaA and LexA in an independent manner, but the two proteins act in an additive manner during the SOS response Wurihan,2018
Evidence: [COMP-AINF] Inferred computationally without human oversight
Promoter
Name: recNp
+1: 2751759
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 36
Sequence: aattattctaattttacgccagcctctttactgtatataaaaccagtttatactgtacacAataacagtaatggtttttca
                           -35                   -10        +1                   
Evidence: [COMP-AINF]
[COMP-HINF-POSITIONAL-IDENTIFICATION]
Reference(s): [1] Huerta AM., et al., 2003
[2] Rostas K., et al., 1987
TF binding sites (TFBSs)
[DnaA,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence Confidence level (C: Confirmed, S: Strong, W: Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal DnaA-ATP repressor recNp 2751730 2751738 -25.0 gcctctttacTGTATATAAaaccagttta nd [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [COMP] W [3]
proximal DnaA-ATP repressor recNp 2751751 2751759 -4.0 ccagtttataCTGTACACAataacagtaa nd [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [COMP] W [3]
[LexA,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence Confidence level (C: Confirmed, S: Strong, W: Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal LexA repressor recNp 2751727 2751746 -22.5 ccagcctcttTACTGTATATAAAACCAGTTtatactgtac nd [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [COMP-AINF-SIMILAR-TO-CONSENSUS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] W [2], [3]
proximal LexA repressor recNp 2751749 2751768 -0.5 aaccagtttaTACTGTACACAATAACAGTAatggtttttc nd [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [COMP-AINF-SIMILAR-TO-CONSENSUS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] W [2], [3]


Evidence    

 [COMP-AINF] Inferred computationally without human oversight

 [COMP-HINF-POSITIONAL-IDENTIFICATION] Human inference of promoter position

 [EXP-IEP-GENE-EXPRESSION-ANALYSIS] Gene expression analysis

 [COMP] Inferred by computational analysis

 [COMP-AINF-SIMILAR-TO-CONSENSUS] Automated inference based on similarity to consensus sequences

 [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] Binding of purified proteins


Reference(s)    

 [1] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [2] Rostas K., Morton SJ., Picksley SM., Lloyd RG., 1987, Nucleotide sequence and LexA regulation of the Escherichia coli recN gene., Nucleic Acids Res 15(13):5041-9

 [3] Wurihan., Gezi., Brambilla E., Wang S., Sun H., Fan L., Shi Y., Sclavi B., Morigen., 2018, DnaA and LexA Proteins Regulate Transcription of the uvrB Gene in Escherichia coli: The Role of DnaA in the Control of the SOS Regulon., Front Microbiol 9:1212

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