RegulonDB RegulonDB 10.9: Operon Form
   

fliE operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: fliE
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: fliE
Gene(s): fliE   Genome Browser M3D Gene expression COLOMBOS
Note(s): In a study where it was shown that the L-tryptophan eradicated persister cells, it was observed that the exogenous amino acid downregulated the expression of fliE as well as other flagellar genes 31271063.
Evidence: [AISGDTU] Automated inference that a single-gene directon is a transcription unit
Reference(s): [1] Stafford GP., et al., 2005
Promoter
Name: fliEp1
+1: 2013036
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 22
Sequence: cgtttttttgttgctatttagcgcctttgtcttattgacttactggtaggctttgctaccAgaaattatccgggagacgag
                          -35                  -10          +1                   
Note(s): We assigned a putative transcriptional start site to this promoter based on the observation that the majority of the promoters, whose transcriptional start sites were determined experimentally, are present at a distance of 6 nucleotides between the transcriptional start site and the -10 box.
In a cell-free expression system, FlhDC slightly activated fliE expression while σ28 did not show an effect on fliE expression 27186988
Evidence: [HIPP]
[ICWHO]
Reference(s): [2] Huerta AM., et al., 2003
[1] Stafford GP., et al., 2005
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal CsgD repressor fliEp1 2013054 2013068 -24.5 tagcgcctttGTCTTATTGACTTACtggtaggctt nd [APIORCISFBSCS], [BPP], [GEA], [APIORCISFBSCS], [BPP], [GEA] [4]
proximal CsgD repressor fliEp1 2013069 2013083 -39.5 ttttttgttgCTATTTAGCGCCTTTgtcttattga nd [APIORCISFBSCS], [BPP], [GEA], [APIORCISFBSCS], [BPP], [GEA] [4]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal FlhDC activator fliEp1 2013068 2013086 -40.5 cgtttttttgTTGCTATTTAGCGCCTTTGtcttattgac nd [APIORCISFBSCS], [BPP], [GEA] [1], [3]
proximal FlhDC activator fliEp1 2013097 2013113 -69.0 ttcgttttttTCTATCCGCCAATAAACCcgtttttttg nd [APIORCISFBSCS], [BPP], [GEA] [1], [3]


Transcription unit          
Name: fliE
Gene(s): fliE   Genome Browser M3D Gene expression COLOMBOS
Note(s): In a study where it was shown that the L-tryptophan eradicated persister cells, it was observed that the exogenous amino acid downregulated the expression of flgBCDEFGHIJ as well as other flagellar genes 31271063.
Evidence: [AISGDTU] Automated inference that a single-gene directon is a transcription unit
[BTEI] Boundaries of transcription experimentally identified
Reference(s): [5] Muller V., et al., 1992
Promoter
Name: fliEp
+1: 2013093
Sigma Factor: Sigma28 Sigmulon
Distance from start of the gene: 79
Sequence: gaaaaattgacgctcccattatttgtgctttcgtttttttctatccgccaataaacccgtTtttttgttgctatttagcgc
                          -35                  -10          +1                   
Note(s): We assigned a putative transcription start site to this promoter based on the observation that the majority of the
promoters, whose transcription start sites were determined experimentally, present a distance of 6 nucleotides between
the transcription start site and the -10 box.
Evidence: [HIPP]
[ICWHO]
Reference(s): [2] Huerta AM., et al., 2003
[5] Muller V., et al., 1992
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote CsgD repressor fliEp 2013054 2013068 33.5 tagcgcctttGTCTTATTGACTTACtggtaggctt nd [APIORCISFBSCS], [BPP], [GEA], [APIORCISFBSCS], [BPP], [GEA] [4]
proximal CsgD repressor fliEp 2013069 2013083 18.5 ttttttgttgCTATTTAGCGCCTTTgtcttattga nd [APIORCISFBSCS], [BPP], [GEA], [APIORCISFBSCS], [BPP], [GEA] [4]





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