RegulonDB RegulonDB 10.9: Operon Form
   

dcuSR operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: dcuSR
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: dcuR
Gene(s): dcuR   Genome Browser M3D Gene expression COLOMBOS
Promoter
Name: dcuRp
+1: 4350565
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 531
Sequence: acgatcccacgaatttatgaataaattgcatgtgattctcggattattgcatctgaagagTtataagcagttggaagatta
                  -35                        -10            +1                   
Note(s): The dcuRp promoter is positively regulated by CRP-cAMP under both aerobic and anaerobic conditions Oyamada T,2007.
Evidence: [ICWHO]
[TIM]
Reference(s): [1] Huerta AM., et al., 2003
[2] Oyamada T., et al., 2007
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal CRP-cAMP activator dcuRp 4350612 4350633 -57.5 atgctgacgcACTTCGTGAACGATCCCACGAATttatgaataa nd [APIORCISFBSCS], [BPP] [2]


Transcription unit          
Name: dcuSR
Gene(s): dcuR, dcuS   Genome Browser M3D Gene expression COLOMBOS
Note(s): The dcuSR operon is constitutively expressed in the absence of nitrate Oyamada T,2007 however, when nitrate is present, the operon is regulated by NarL. NarL binds to a region from positions -25 to -75, upstream the transcriptional start site of dcuS, where there are two consensus sequences. These sequences were identified as the NarL-binding site Goh EB,2005
Based on β-galactosidase and Western blot analyses, it was shown that the expression of the dcuSR operon is positively regulated during the growth phase but it is not σS dependent 19789137 In addition, the results suggest that other growth phase-dependent regulatory mechanisms, such as DcuSR and CRP, could be involved during postexponential dcuSR expression 19789137
Evidence: [ITCR] Inferred through co-regulation
Reference(s): [3] Salmon K., et al., 2003
Promoter
Name: dcuSp
+1: 4351685
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 23
Sequence: atatagccaatcattcttactccctttgaattacccgcctcatcagagataatgcttaagAaatttgtcacacaaggaagc
                          -35                   -10         +1                   
Evidence: [HIPP]
[ICWHO]
[TIM]
Reference(s): [4] Goh EB., et al., 2005
[1] Huerta AM., et al., 2003
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd FNR1 activator dcuSp nd nd nd nd nd [BPP], [GEA], [IGI] [3]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal NarL-Phosphorylated repressor dcuSp 4351713 4351719 -31.0 cttactccctTTGAATTAcccgcctcat nd [APIORCISFBSCS], [BPP], [GEA] [4]
proximal NarL-Phosphorylated repressor dcuSp 4351722 4351728 -40.0 ccaatcattcTTACTCCCtttgaattac nd [APIORCISFBSCS], [BPP], [GEA] [4]
Note(s): 1By making use of microarray analyses, it was concluded that FNR activates dcuSR operon expression under anaerobiosis. A putative FNR-binding site, which was not shown in the paper, was identified upstream of this operon Salmon K,2003.3By making use of microarray analyses, it was concluded that FNR activates dcuSR operon expression under anaerobiosis. A putative FNR-binding site, which was not shown in the paper, was identified upstream of this operon Salmon K,2003.





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