RegulonDB RegulonDB 11.0: Operon Form
   

dcuSR operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: dcuSR
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: dcuR
Gene(s): dcuR   Genome Browser M3D Gene expression COLOMBOS
Promoter
Name: dcuRp
+1: 4350565
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 531
Sequence: acgatcccacgaatttatgaataaattgcatgtgattctcggattattgcatctgaagagTtataagcagttggaagatta
                  -35                        -10            +1                   
Note(s): The dcuRp promoter is positively regulated by CRP-cAMP under both aerobic and anaerobic conditions Oyamada T,2007.
Evidence: [ICWHO]
[TIM]
Reference(s): [1] Huerta AM., et al., 2003
[2] Oyamada T., et al., 2007
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal CRP-cyclic-AMP activator dcuRp 4350612 4350633 -57.5 tgctgacgcaCTTCGTGAACGATCCCACGAATttatgaataa nd [APIORCISFBSCS], [BPP] [2]


Transcription unit          
Name: dcuSR
Gene(s): dcuR, dcuS   Genome Browser M3D Gene expression COLOMBOS
Note(s): The dcuSR operon is constitutively expressed in the absence of nitrate Oyamada T,2007 however, when nitrate is present, the operon is regulated by NarL. NarL binds to a region from positions -25 to -75, upstream the transcriptional start site of dcuS, where there are two consensus sequences. These sequences were identified as the NarL-binding site Goh EB,2005
Based on β-galactosidase and Western blot analyses, it was shown that the expression of the dcuSR operon is positively regulated during the growth phase but it is not σS dependent Abo-Amer AE, Altalhi AD,2009 In addition, the results suggest that other growth phase-dependent regulatory mechanisms, such as DcuSR and CRP, could be involved during postexponential dcuSR expression Abo-Amer AE, Altalhi AD,2009
Evidence: [ITCR] Inferred through co-regulation
Reference(s): [3] Salmon K., et al., 2003
Promoter
Name: dcuSp
+1: 4351685
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 23
Sequence: atatagccaatcattcttactccctttgaattacccgcctcatcagagataatgcttaagAaatttgtcacacaaggaagc
                          -35                   -10         +1                   
Evidence: [HIPP]
[ICWHO]
[TIM]
Reference(s): [4] Goh EB., et al., 2005
[1] Huerta AM., et al., 2003
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd FNR activator dcuSp nd nd nd nd nd [GEA], [BPP] [3]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal NarL-phosphorylated repressor dcuSp 4351713 4351719 -31.0 ttactcccttTGAATTAcccgcctcat nd [GEA], [APIORCISFBSCS], [BPP] [4]
proximal NarL-phosphorylated repressor dcuSp 4351722 4351728 -40.0 caatcattctTACTCCCtttgaattac nd [GEA], [APIORCISFBSCS], [BPP] [4]





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