RegulonDB RegulonDB 10.9: Operon Form

ldhA operon and associated TUs in Escherichia coli K-12 genome

Name: ldhA
This page displays every known transcription unit of this operon and their known regulation.

Transcription unit       
Name: ldhA
Gene(s): ldhA   Genome Browser M3D Gene expression COLOMBOS
Note(s): Overexpression of the ldhA gene results in very poor growth because of the excess lactate dehydrogenase produced, which converts the pyruvate pool to lactate and causes a shortage of 3-carbon metabolic intermediates 22430499.
Based on gene expression analysis, the expression of ldhA is regulated by ArcB, Mlc, the CsrAB complex, and GlgA Jiang GR,2001. Bunch PK,1997.
Reference(s): [1] Bunch PK., et al., 1997
[2] Jiang GR., et al., 2001
Name: ldhAp
+1: 1442915
Sigma Factor: Sigma32 Sigmulon
Distance from start of the gene: 72
Sequence: gaaggttgcgcctacactaagcatagttgttgatgaatttttcaatatcgccatagctttCaattaaatttgaaattttgt
                              -35                -10        +1                   
Note(s): The sigma factor was determined by transcription profiling experiments and a bioinformatics approach Zhao K,2005. We assigned a putative transcriptional start site to this promoter based on the observation that the majority of the promoters, whose transcriptional start sites were determined experimentally, present a distance of 6 nucleotides between the transcriptional start site and the -10 box.
Evidence: [AIPP]
Reference(s): [3] Huerta AM., et al., 2003
[2] Jiang GR., et al., 2001
[4] Wade JT., et al., 2006
[5] Zhao K., et al., 2005