|Gene(s):||yegZ, yegR Genome Browser M3D Gene expression COLOMBOS|
|Note(s):||Masuda N,2003proposed a model of the regulatory network of the acid resistance genes, including evgA, ydeP, ydeO, yhiE, gadABC, and hdeAB, using a combination of microarray analysis and gene replacement techniques. With these assays, 7 operons were identified as induced when EvgA is overexpressed and YdeO is absent; on the other hand, 12 operons were identified as induced when YdeO is overexpressed. To confirm these regulatory interactions, the EvgA consensus site was searched with the ALIGNACE program, and the 18-bp site found was tested by gel shift analysis with the upstream regions of the corresponding operons. As a result, just six operons are regulated by EvgA, among which the yegRZ operon is included.
The specific position of the EvgA site has not been annotated because the +1 position has not been identified, but the experimental data are quoted as follows: regulator, EvgA; operon, yegR-b2084-yegZ; position(s), (-64 to -47)* and 2166445.5**;
Evidences: Consensus, Gene Expression Analysis, and Purified Protein Masuda N,2003
* Numbers relative to the start codon of the yegR gene.
** Absolute position on the E. coli genome.
Based on microarray and RT-qPCR analyses, it was determined that the asr, frc, oxc, ydeO, and yegR acid stress genes are significantly upregulated upon exposure to exogenous glutathione (GSH) Goswami M,2018
|Evidence:||[ITC] Inferred through co-regulation|
|Reference(s):|| Masuda N., et al., 2003|
|Sigma Factor:||Sigma70 Sigmulon|
|Distance from start of the gene:||43|
-35 -10 +1
|Reference(s):|| Itou J., et al., 2009|
|Type||Transcription factor||Function||Promoter||Binding Sites||Growth Conditions||Evidence (Confirmed, Strong, Weak)||Reference(s)|
|proximal||EvgA-Phosphorylated||activator||yegRp||2168413||2168430||-72.5||atgtccgtaaTTCCTACTTATGTAGGAAatgttgtaca||nd||[AIBSCS], [BPP], [CV(CHIP-SV/GEA/ROMA)], [CV(GEA/ROMA)], [GEA]||, |
|Transcription factor||Function||Promoter||Binding Sites||Evidence (Confirmed, Strong, Weak)||Reference(s)|
|EvgA-Phosphorylated||activator||yegRp||2168413||2168430||-72.5||atgtccgtaaTTCCTACTTATGTAGGAAatgttgtaca||[AIBSCS], [BPP], [CV(CHIP-SV/GEA/ROMA)], [CV(GEA/ROMA)], [GEA]||, |