RegulonDB RegulonDB 10.9: Operon Form
   

sbmA-yaiW operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: sbmA-yaiW
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: sbmA-yaiW
Gene(s): sbmA, yaiW   Genome Browser M3D Gene expression COLOMBOS
Note(s): sbmA expression was increased in a tolC mutant, and it was completely σE dependent, in a micF-independent manner 20735480 This upregulation could be caused by the alteration in outer membrane permeability, suggesting a potential coparticipation of both TolC and SbmA in order to solve a physiological problem in which the transport of SbmA-specific substrate could be necessary. On the other hand, it seems that sbmA is governed by another alternative regulation pathway, because it is well known that stress stimuli may activate multiple stress responses 20735480
Under NlpE outer membrane lipoprotein overexpression, the transcription of the sbmA gene is increased by CpxR Raivio TL,2013
Evidence: [ITCR] Inferred through co-regulation
Reference(s): [1] Rhodius VA., et al., 2005
Promoter
Name: sbmAp
+1: 396553
Sigma Factor: Sigma24 Sigmulon
Distance from start of the gene: 86
Sequence: tcgaaacaattcttatggtcaggctggcgaactaagcgccttgctatgggtcacaatgggCggtttcatttggctgacgct
                             -35                   -10      +1                   
Note(s): The transcriptional start site of the sbmAp promoter was also determined by Rhodius VA,2005, and it was different by 1 bp from the one characterized by Rezuchova B,2003.
This promoter appears to be induced in two phases after σE induction. It is induced during the first 5 minutes following σE induction, decreased from 5 to 10 minutes, induced from 10 to 15 minutes, and decreased from 15 to 20 minutes Lacoux C,2020.
Evidence: [AIPP]
[HIPP]
[ICWHO]
[TIM]
Reference(s): [2] Huerta AM., et al., 2003
[3] Rezuchova B., et al., 2003
[1] Rhodius VA., et al., 2005
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd CpxR-Phosphorylated1 activator sbmAp nd nd nd nd nd [BPP], [GEA], [IGI] [4]
Note(s): 1The regulation of CpxR on the sbmA-yaiW operon has been probed by quantitative PCR analysis of cDNA and by the identification of a consensus DNA-binding site of CpxR in the regulatory region of sbmA-yaiW Raivio TL,2013 The identification of this DNA-binding site was only mentioned in the paper of Raivio et al. (2013), but the DNA sequence was not shown Raivio TL,20131The regulation of CpxR on the sbmA-yaiW operon has been probed by quantitative PCR analysis of cDNA and by the identification of a consensus DNA-binding site of CpxR in the regulatory region of sbmA-yaiW Raivio TL,2013 The identification of this DNA-binding site was only mentioned in the paper of Raivio et al. (2013), but the DNA sequence was not shown Raivio TL,2013





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