RegulonDB RegulonDB 11.1: Operon Form
   

qseBC operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: qseBC
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: qseBC
Gene(s): qseB, qseC   Genome Browser M3D Gene expression COLOMBOS
Evidence: [EXP-IEP-COREGULATION] Inferred through co-regulation
Reference(s): [1] Clarke MB., et al., 2005
[2] Sperandio V., et al., 2002
Promoter
Name: qseBp2
+1: 3169749
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 79
Sequence: ctttaatgtctgtttccgagcatttaacaagatagtccttaacaacttcttaagggaaaaAaataaaatttagtgctgtac
Evidence: [COMP-AINF]
[EXP-IDA]
Reference(s): [3] Imashimizu M., et al., 2020
TF binding sites (TFBSs)
[BasR,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence Confidence level (C: Confirmed, S: Strong, W: Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal BasR-phosphorylated activator qseBp2 3169726 3169745 -13.5 caagatagtcCTTAACAACTTCTTAAGGGAaaaaaataaa nd [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] W [4]
[QseB,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence Confidence level (C: Confirmed, S: Strong, W: Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote QseB-Phosphorylated activator qseBp2 3169281 3169293 -462.0 ggagcggtaaCAATTACGGATTTactttgcgga nd [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] W [1]
proximal QseB-Phosphorylated activator qseBp2 3169748 3169760 6.0 ttaagggaaaAAAATAAAATTTAgtgctgtaca nd [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] W [1]


Transcription unit       
Name: qseBC
Gene(s): qseB, qseC   Genome Browser M3D Gene expression COLOMBOS
Evidence: [EXP-IEP-COREGULATION] Inferred through co-regulation
Reference(s): [1] Clarke MB., et al., 2005
[2] Sperandio V., et al., 2002
Promoter
Name: qseBp1
+1: 3169801
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 27
Sequence: agggaaaaaaataaaatttagtgctgtacagagcgcgttacaacacggtttactggcagcAaatacggttatcgcagggat
                        -35                        -10      +1                   
Evidence: [COMP-AINF]
[EXP-IDA-TRANSCRIPTION-INIT-MAPPING]
Reference(s): [1] Clarke MB., et al., 2005
[5] Huerta AM., et al., 2003


Evidence    

 [COMP-AINF] Inferred computationally without human oversight

 [EXP-IDA] Inferred from direct assay

 [EXP-IEP-GENE-EXPRESSION-ANALYSIS] Gene expression analysis

 [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] Binding of purified proteins

 [EXP-IDA-TRANSCRIPTION-INIT-MAPPING] Transcription initiation mapping


Reference(s)    

 [1] Clarke MB., Sperandio V., 2005, Transcriptional autoregulation by quorum sensing Escherichia coli regulators B and C (QseBC) in enterohaemorrhagic E. coli (EHEC)., Mol Microbiol 58(2):441-55

 [2] Sperandio V., Torres AG., Kaper JB., 2002, Quorum sensing Escherichia coli regulators B and C (QseBC): a novel two-component regulatory system involved in the regulation of flagella and motility by quorum sensing in E. coli., Mol Microbiol 43(3):809-21

 [3] Imashimizu M., Tokunaga Y., Afek A., Takahashi H., Shimamoto N., Lukatsky DB., 2020, Control of Transcription Initiation by Biased Thermal Fluctuations on Repetitive Genomic Sequences., Biomolecules 10(9)

 [4] Guckes KR., Kostakioti M., Breland EJ., Gu AP., Shaffer CL., Martinez CR., Hultgren SJ., Hadjifrangiskou M., 2013, Strong cross-system interactions drive the activation of the QseB response regulator in the absence of its cognate sensor., Proc Natl Acad Sci U S A 110(41):16592-7

 [5] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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