|
|
| Operon |  |
|---|---|
| Name: | hisLGDCBHAFI |
| This page displays every known transcription unit of this operon and their known regulation. | |
| Name: | hisLGDCBHAFI |
| Gene(s): | hisL, hisG, hisD, hisC, hisB, hisH, hisA, hisF, hisI Genome Browser M3D Gene expression COLOMBOS |
| Note(s): | The hisLGDCBHAFI operon codes for the enzymes of the histidine biosynthesis pathway. It is controlled by attenuation in response to histidine availability. When histidine is abundant, the ribosome quickly translates through a stretch of seven histidine codons in the hisL leader region, allowing formation of the transcriptional terminator between hisL and hisG. In contrast, when histidine is not available, the ribosome stalls within the leader region and the terminator will not form, allowing transcription to continue Di Nocera PP,1978. Verde P,1981. 6166940. |
| Evidence: | [BTEI] Boundaries of transcription experimentally identified [ITCR] Inferred through co-regulation |
| Reference(s): |
[1] Alifano P., et al., 1992 [2] Goldschmidt EP., et al., 1970 |
| Promoter | |
| Name: | hisLp |
| +1: | 2089965 |
| Distance from start of the gene: | 31 |
| Sequence: |
ccataaaatatataaaaaagcccttgctttctaacgtgaaagtggtttaggttaaaagacAtcagttgaataaacattcac |
| Note(s): | Guanosine tetraphosphate (ppGpp) increases the transcription of the hisLp promoter. In contrast, ppGpp reduces the transcription of other promoters, such as argTp and rrnBp1. |
| Evidence: |
[CV(RPF/RS-EPT-CBR)] [HIPP] [RPF] [RS-EPT-CBR] |
| Reference(s): |
[3] Salgado H, et al., 2012 [4] Verde P., et al., 1981 |
| Evidence: | [IDA] Inferred from direct assay |
| Reference(s): | [5] Lee JH., et al., 2012 |
| Name: | hisL |
| Gene(s): | hisL Genome Browser M3D Gene expression COLOMBOS |
| Evidence: | [BTEI] Boundaries of transcription experimentally identified |
| Reference(s): |
[6] Di Nocera PP., et al., 1978 [4] Verde P., et al., 1981 |
| Promoter | |
| Name: | hisLp |
| +1: | 2089965 |
| Distance from start of the gene: | 31 |
| Sequence: |
ccataaaatatataaaaaagcccttgctttctaacgtgaaagtggtttaggttaaaagacAtcagttgaataaacattcac |
| Note(s): | Guanosine tetraphosphate (ppGpp) increases the transcription of the hisLp promoter. In contrast, ppGpp reduces the transcription of other promoters, such as argTp and rrnBp1. |
| Evidence: |
[CV(RPF/RS-EPT-CBR)] [HIPP] [RPF] [RS-EPT-CBR] |
| Reference(s): |
[3] Salgado H, et al., 2012 [4] Verde P., et al., 1981 |
| Terminator(s) | |
| Type: | rho-independent |
| Sequence: | aacgcatgagAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTtttattgcgc |
| Reference(s): | [6] Di Nocera PP., et al., 1978 |
| Evidence: | [IDA] Inferred from direct assay |
| Reference(s): | [5] Lee JH., et al., 2012 |
| Name: | hisBHAFI |
| Gene(s): | hisB, hisH, hisA, hisF, hisI Genome Browser M3D Gene expression COLOMBOS |
| Note(s): | The hisA gene is upregulated by long-term (8 to 12 h) exposure of E. coli to some biocides 32385082. |
| Evidence: | [BTEI] Boundaries of transcription experimentally identified |
| Reference(s): | [7] Grisolia V., et al., 1983 |
| Promoter | |
| Name: | hisBp |
| +1: | 2093360 |
| Sigma Factor: | Sigma70 Sigmulon |
| Distance from start of the gene: | 108 |
| Sequence: |
ggcgcgctttaaagcctccagtgcggtgtttaaatctttgtgggatcagggcattatcttAcgtgatcagaataaacaacc -35 -10 +1 |
| Evidence: |
[ICWHO] [TIM] |
| Reference(s): |
[7] Grisolia V., et al., 1983 [8] Huerta AM., et al., 2003 |
| RNA cis-regulatory element | |
|---|
| Regulation, transcriptional elongation | |
| Attenuator type: | Transcriptional |
| Strand: | forward |
| Evidence: | [ICA] Inferred by computational analysis |
| Reference(s): | [9] Merino E, et al., 2005 |
| Structure type | Energy | LeftPos | RightPos | Sequence (RNA-strand) | |
|---|---|---|---|---|---|
| terminator | -27.0 | 2090108 | 2090146 | acgcatgagaAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTgcgcggttga | |
| anti-terminator | -16.9 | 2090079 | 2090124 | agacattcagATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCaccttccggg | |
| anti-anti-terminator | -16.3 | 2090050 | 2090099 | gactagtcttTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAAcgcatgagaa |
| Notes: "The provided "Sequence" is that of the RNA strand, i.e. U's are shown instead of T's and regulators on the reverse strand will appear as the reverse complement of the sequence delimited by LeftPos-RigtPos" |
| REGULATION, RNA cis-regulatory element: | |
| Rfam type: | Cis-reg; leader |
| Strand: | forward |
| Evidence: | [ICA] Inferred by computational analysis |
| Reference(s): | [10] null null |
| Description | Rfam score | Left Pos | Right Pos | Sequence (RNA-strand) | |
|---|---|---|---|---|---|
| Histidine operon leader | 131.6 | 2090022 | 2090145 | uuaaacaccaCCAUCAUCACCAUCAUCCUGACUAGUCUUUCAGGCGAUGUGUGCUGGAAGACAUUCAGAUCUUCCAGUGGUGCAUGAACGCAUGAGAAAGCCCCCGGAAGAUCACCUUCCGGGGGCUUUUUUAUugcgcgguug |
| Notes: "The provied "Sequence" is that of the RNA strand, i.e. U's are shown instead of T's and regulators on the reverse strand will appears as the reverse complement of the sequence delimited by LeftPos-RightPos" |
| Reference(s) |
|
|---|---|
[CV(RPF/RS-EPT-CBR)] cross validation(RPF/RS-EPT-CBR)