RegulonDB RegulonDB 10.9: Operon Form

yhdT-panF-prmA operon and associated TUs in Escherichia coli K-12 genome

Name: yhdT-panF-prmA
This page displays every known transcription unit of this operon and their known regulation.

Transcription unit       
Name: yhdT-panF-prmA
Gene(s): yhdT, panF, prmA   Genome Browser M3D Gene expression COLOMBOS
Note(s): The yhdT gene was not identified by Vanet et al. Vanet A,1993, although it is located just downstream of the promoter that they identified and which transcribes the panF and prmA genes. Therefore, we included the gene (yhdT) in the operon.
It was suggested that panF and prmA are translationally coupled Vanet A,1993.
Evidence: [BTEI] Boundaries of transcription experimentally identified
[PM] Polar mutation
Reference(s): [1] Vanet A., et al., 1993
Name: yhdTp
+1: 3407351
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 24
Sequence: gattttccggccttttttattactggggatcgacaacccccataaggtacaatccccgctTtcttcacccatcagggacaa
                       -35                      -10         +1                   
Note(s): A -10 box was identified upstream of the transcription start site of this promoter, whereas a -35 box was not.
Evidence: [ICWHO]
Reference(s): [2] Huerta AM., et al., 2003
[1] Vanet A., et al., 1993
Type: rho-independent
Sequence: ccgattatttACGCAAATTTGCGTgccaaaattt
Reference(s): [1] Vanet A., et al., 1993

RNA cis-regulatory element    
Regulation, transcriptional elongation  
Attenuator type: Transcriptional
Strand: forward
  Structure type Energy LeftPos RightPos Sequence (RNA-strand)
  terminator -15.6 3407283 3407311 aaagcgtcaaAAGGCCGGATTTTCCGGCCTTTTTTATTactggggatc
  anti-terminator -9.84 3407247 3407291 agaaaaaactCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGattttccggc
  anti-anti-terminator -4.6 3407248 3407271 gaaaaaactcGGTCTTCAGGAAAAATAAGACTGctaaagcgtc
Notes: "The provided "Sequence" is that of the RNA strand, i.e. U's are shown instead of T's and regulators on the reverse strand will appear as the reverse complement of the sequence delimited by LeftPos-RigtPos"