RegulonDB RegulonDB 11.1: Operon Form
   

purT operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: purT
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: purT
Gene(s): purT   Genome Browser M3D Gene expression COLOMBOS
Note(s): Based on a combination of genetic, functional, and biochemical analyses, regulatory elements were identified in the regulatory region of purT Belliveau NM,2018
Evidence: [COMP-AINF-SINGLE-DIRECTON] Automated inference that a single-gene directon is a transcription unit
Promoter
Name: purTp
+1: 1930857
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 24
Sequence: gttgagaaatcaacatcagcaataaagacacacgcaaacgttttcgtttatactgcgcgcGgaattaatcaggggatattc
                         -35                     -10        +1                   
Evidence: [COMP-AINF]
[COMP-AINF-PATTERN-DISCOVERY]
[EXP-IMP]
[IC]
Reference(s): [1] Belliveau NM., et al., 2018
TF binding sites (TFBSs)
[PurR,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence Confidence level (C: Confirmed, S: Strong, W: Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal PurR-hypoxanthine repressor purTp 1930828 1930844 -21.5 ataaagacacACGCAAACGTTTTCGTTtatactgcgc nd [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [COMP-AINF-SIMILAR-TO-CONSENSUS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS], [EXP-IMP-SITE-MUTATION] C [1]


Evidence    

 [COMP-AINF] Inferred computationally without human oversight

 [COMP-AINF-PATTERN-DISCOVERY] Automated inference based on sequence pattern discovery

 [EXP-IMP] Inferred from mutant phenotype

 [IC] Inferred by curator

 [EXP-IEP-GENE-EXPRESSION-ANALYSIS] Gene expression analysis

 [COMP-AINF-SIMILAR-TO-CONSENSUS] Automated inference based on similarity to consensus sequences

 [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] Binding of purified proteins

 [EXP-IMP-SITE-MUTATION] Site mutation


Reference(s)    

 [1] Belliveau NM., Barnes SL., Ireland WT., Jones DL., Sweredoski MJ., Moradian A., Hess S., Kinney JB., Phillips R., 2018, Systematic approach for dissecting the molecular mechanisms of transcriptional regulation in bacteria., Proc Natl Acad Sci U S A 115(21):E4796-E4805

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