RegulonDB RegulonDB 10.9: Operon Form
   

luxS operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: luxS
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: luxS
Gene(s): luxS   Genome Browser M3D Gene expression COLOMBOS
Note(s): Udekwu, in 2010, identified several transcription start sites upstream of luxS; however, he considered that only two promoters are the producers of primary transcripts Udekwu KI.,2010 One of them was also identified by De Lay (2009) De Lay N,2009
The transcription start site for luxS lies within the divergent gene for
micA. The luxS mRNA and the MicA sRNA share 15 bp of complementary
sequence and may facilitate posttranscriptional regulation of luxS by Mi
cA De Lay N,2009
The transcription of the luxS gene is repressed after 24 h of coculture with microcapsules of Lactobacillus rhamnosus 31147809.
Evidence: [AISGDTU] Automated inference that a single-gene directon is a transcription unit
[LTED] Length of transcript experimentally determined
Reference(s): [1] De Lay N., et al., 2009
Promoter
Name: luxSp2
+1: 2814816
Distance from start of the gene: 83
Sequence: agaaaaaggccactcgtgagtggccaaaatttcatctctgaattcagggatgatgataacAaatgcgcgtctttcatatac
Evidence: [RS-EPT-CBR]
[TIM]
Reference(s): [1] De Lay N., et al., 2009
[2] Salgado H, et al., 2012
[3] Udekwu KI. 2010
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd UvrY-Phosphorylated activator luxSp2 nd nd nd nd nd [BPP], [GEA], [IGI] [4]
sRNA Interaction TU
sRNA TU Regulated Function Binding Sites Regulatory Mechanism Evidence (Confirmed, Strong, Weak) Reference(s)
PosLeft PosRight Target sequence (mRNA)
small regulatory RNA CyaR luxS repressor 2814731 2814745 CAUUUAGCCACCUCC nd [IEP], [SM] [1]


Transcription unit       
Name: luxS
Gene(s): luxS   Genome Browser M3D Gene expression COLOMBOS
Note(s): Udekwu, in 2010, identified several transcription start sites upstream of luxS; however, he considered that only two promoters are the producers of primary transcripts Udekwu KI.,2010 One of them was also identified by De Lay (2009) De Lay N,2009.
The transcription of the luxS gene is repressed after 24 h of coculture with microcapsules of Lactobacillus rhamnosus 31147809.
Evidence: [LTED] Length of transcript experimentally determined
Reference(s): [1] De Lay N., et al., 2009
Promoter
Name: luxSp1
+1: 2815065
Sigma Factor: Sigma38 Sigmulon
Distance from start of the gene: 332
Sequence: tgcttcgataatcccgatctgactttctctgcccgtatcttaaggtctatgattgatactGgtattggcggaacaggcaaa
                          -35              -10              +1                   
Note(s): The activity of the luxSp1 promoter is increased during entry into stationary phase and appears to depend on σ38 Udekwu KI.,2010.
The DNA sequence of the luxSp1 promoter of E. coli is conserved in Shigella flexneri, Salmonella typhimurium, and Photorabdus luminescens Udekwu KI.,2010.
Evidence: [HIPP]
[ICWHO]
[TIM]
Reference(s): [5] Huerta AM., et al., 2003
[3] Udekwu KI. 2010





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