RegulonDB RegulonDB 10.9: Operon Form
   

aslB operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: aslB
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: aslB
Gene(s): aslB   Genome Browser M3D Gene expression COLOMBOS
Promoter
Name: aslBp
+1: 3982781
Sigma Factor: Sigma54 Sigmulon
Distance from start of the gene: 177
Sequence: aaaaagcagtatttcggcgagtagcgcagcttggtagcgcaactggtttgggaccagtggGtcggaggttcgaatcctctc
                                -24         -12             +1                   
Note(s): This promoter was identified by microarray assays with a mutant σ54 strain and a σ54-overexpressing strain, and by σ54-related promoter element identification Zhao K,2010
We assigned a putative transcription start site to this promoter based on the observation that the majority of the σ54-dependent promoters, whose transcription start sites were determined experimentally, present a distance of 11 nucleotides between the transcription start site and the -12 box Barrios H,1999.
Evidence: [AIPP]
[ICWHO]
[IEP]
Reference(s): [1] Huerta AM., et al., 2003
[2] Zhao K., et al., 2010
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd GadE-RcsB activator aslBp nd nd nd nd nd [BPP], [GEA], [IGI] [4]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd Rob activator aslBp nd nd nd nd nd [BPP], [GEA], [IGI] [3]


RNA cis-regulatory element    
Regulation, transcriptional elongation  
Attenuator type: Transcriptional
Strand: forward
  Structure type Energy LeftPos RightPos Sequence (RNA-strand)
  terminator -19.0 3982810 3982848 ctcgccgaccAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTTCTgtgcatttcg
  anti-terminator -13.8 3982780 3982826 ggaccagtggGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTtcggcggggt
  anti-anti-terminator -5.8 3982779 3982803 gggaccagtgGGTCGGAGGTTCGAATCCTCTCTCgccgaccaat
Notes: "The provided "Sequence" is that of the RNA strand, i.e. U's are shown instead of T's and regulators on the reverse strand will appear as the reverse complement of the sequence delimited by LeftPos-RigtPos"





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